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Open data
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Basic information
Entry | Database: PDB / ID: 5dmd | |||||||||
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Title | Crystal structure of the flagellar assembly factor FliW | |||||||||
![]() | Flagellar assembly factor FliW | |||||||||
![]() | TRANSLATION / flagellum / assembly factor | |||||||||
Function / homology | ![]() bacterial-type flagellum assembly / regulation of translation / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Altegoer, F. / Bange, G. | |||||||||
![]() | ![]() Title: Structural basis for the CsrA-dependent modulation of translation initiation by an ancient regulatory protein. Authors: Altegoer, F. / Rensing, S.A. / Bange, G. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 155.7 KB | Display | ![]() |
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PDB format | ![]() | 103 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 428.7 KB | Display | ![]() |
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Full document | ![]() | 430.3 KB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Data in CIF | ![]() | 21.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5dmbC ![]() 5jakC ![]() 2aj7S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17389.801 Da / Num. of mol.: 2 / Mutation: P40Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: fliW, GTNG_3059 / Plasmid: pET24d(+) / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.86 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.1 M MES pH 6.0, 40 % (v/v) PEG 400, 5 % (w/v) PEG 3000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Mar 6, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 1.45→46.38 Å / Num. obs: 49625 / % possible obs: 99.4 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.056 / Net I/σ(I): 9.5 |
Reflection shell | Resolution: 1.45→1.53 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.555 / Mean I/σ(I) obs: 1.9 / % possible all: 96.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2AJ7 Resolution: 1.45→46.383 Å / SU ML: 0.17 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 23.44 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.45→46.383 Å
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Refine LS restraints |
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LS refinement shell |
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