[English] 日本語
![](img/lk-miru.gif)
- PDB-5che: Crystal structure of Arabidopsis glutamyl-tRNA reductase in compl... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 5che | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of Arabidopsis glutamyl-tRNA reductase in complex with its regulatory proteins | ||||||
![]() |
| ||||||
![]() | OXIDOREDUCTASE / GluTR / tertiary complex / regulatory proteins / anchor protein | ||||||
Function / homology | ![]() glutamyl-tRNA reductase / glutamyl-tRNA reductase activity / tetrapyrrole biosynthetic process / positive regulation of heme biosynthetic process / chloroplast membrane / chlorophyll biosynthetic process / chloroplast thylakoid / protoporphyrinogen IX biosynthetic process / photosynthetic electron transport chain / heme biosynthetic process ...glutamyl-tRNA reductase / glutamyl-tRNA reductase activity / tetrapyrrole biosynthetic process / positive regulation of heme biosynthetic process / chloroplast membrane / chlorophyll biosynthetic process / chloroplast thylakoid / protoporphyrinogen IX biosynthetic process / photosynthetic electron transport chain / heme biosynthetic process / chloroplast stroma / chloroplast thylakoid membrane / protein-membrane adaptor activity / post-embryonic development / chloroplast / NADP binding / protein-containing complex Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Fang, Y. / Liu, L. | ||||||
![]() | ![]() Title: The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein Authors: Fang, Y. / Zhao, S. / Zhang, F. / Zhao, A. / Zhang, W. / Zhang, M. / Liu, L. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 317.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 252.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 486.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 504.5 KB | Display | |
Data in XML | ![]() | 53.4 KB | Display | |
Data in CIF | ![]() | 72.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 52139.902 Da / Num. of mol.: 2 / Fragment: UNP residues 73-543 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 34272.648 Da / Num. of mol.: 2 / Fragment: UNP residues 42-317 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Protein | Mass: 17771.264 Da / Num. of mol.: 2 / Fragment: UNP residues 195-316 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.09 % |
---|---|
Crystal grow | Temperature: 289 K / Method: evaporation / pH: 7 / Details: PEG 3350, sodium malonate, LiCl, MPD |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-325 / Detector: CCD / Date: Jan 19, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→50 Å / Num. all: 36775 / Num. obs: 36775 / % possible obs: 99.6 % / Redundancy: 4.3 % / Rmerge(I) obs: 0.132 / Net I/σ(I): 10 |
Reflection shell | Resolution: 3.2→3.31 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.831 / Mean I/σ(I) obs: 1.68 / % possible all: 99.9 |
-Phasing
Phasing | Method: ![]() |
---|
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 4N7R, 4YVQ Resolution: 3.203→39.265 Å / FOM work R set: 0.7788 / SU ML: 0.45 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.67 / Stereochemistry target values: ML
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 108.18 Å2 / Biso mean: 26.75 Å2 / Biso min: 1.43 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.203→39.265 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 12
|