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Yorodumi- PDB-5c9h: Structural Basis of Template Boundary Definition in Tetrahymena T... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5c9h | |||||||||
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Title | Structural Basis of Template Boundary Definition in Tetrahymena Telomerase | |||||||||
Components |
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Keywords | RNA binding protein/RNA / Telomerase / RNA-Protein complex / RNA binding protein-RNA complex | |||||||||
Function / homology | Function and homology information telomerase RNA reverse transcriptase activity / telomerase RNA binding / telomerase holoenzyme complex / telomeric DNA binding / telomere maintenance via telomerase / RNA-directed DNA polymerase / chromosome, telomeric region / metal ion binding Similarity search - Function | |||||||||
Biological species | Tetrahymena thermophila (eukaryote) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | |||||||||
Authors | Jansson, L.I. / Akiyama, B.M. / Ooms, A. / Lu, C. / Rubin, S.M. / Stone, M.D. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2015 Title: Structural basis of template-boundary definition in Tetrahymena telomerase. Authors: Jansson, L.I. / Akiyama, B.M. / Ooms, A. / Lu, C. / Rubin, S.M. / Stone, M.D. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5c9h.cif.gz | 261.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5c9h.ent.gz | 206.3 KB | Display | PDB format |
PDBx/mmJSON format | 5c9h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c9/5c9h ftp://data.pdbj.org/pub/pdb/validation_reports/c9/5c9h | HTTPS FTP |
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-Related structure data
Related structure data | 2r4gS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 36765.578 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Tetrahymena thermophila (eukaryote) / Gene: TERT / Production host: Escherichia coli (E. coli) / References: UniProt: O77448, RNA-directed DNA polymerase #2: RNA chain | Mass: 4103.469 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Tetrahymena thermophila (eukaryote) #3: RNA chain | Mass: 4017.366 Da / Num. of mol.: 2 / Mutation: A10U / Source method: obtained synthetically / Source: (synth.) Tetrahymena thermophila (eukaryote) #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.51 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 200mM KCl, 10mM MgCl2, 50mM MES monohydrate pH 5.6, 4% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.1 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 8, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→41.512 Å / Num. obs: 18728 / % possible obs: 99.5 % / Redundancy: 6.8 % / Rmerge(I) obs: 0.231 / Net I/σ(I): 7.8 |
Reflection shell | Resolution: 3→3.16 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.969 / Mean I/σ(I) obs: 2.1 / % possible all: 99.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2R4G Resolution: 3→41.512 Å / SU ML: 0.42 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.55 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→41.512 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 62.1211 Å / Origin y: 32.7153 Å / Origin z: 145.0066 Å
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Refinement TLS group | Selection details: all |