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- PDB-5c0x: Structure of a 12-subunit nuclear exosome complex bound to struct... -

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Basic information

Entry
Database: PDB / ID: 5c0x
TitleStructure of a 12-subunit nuclear exosome complex bound to structured RNA
Components
  • (Exosome complex component ...) x 9
  • (Exosome complex exonuclease ...) x 2
  • RNA synthetic
Keywordshydrolase/RNA / hydrolase / RNA / nuclease / protein-RNA complex / hydrolase-RNA complex
Function / homology
Function and homology information


Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / mRNA decay by 3' to 5' exoribonuclease / U1 snRNA 3'-end processing / nuclear polyadenylation-dependent CUT catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / U5 snRNA 3'-end processing ...Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / mRNA decay by 3' to 5' exoribonuclease / U1 snRNA 3'-end processing / nuclear polyadenylation-dependent CUT catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / U5 snRNA 3'-end processing / regulatory ncRNA 3'-end processing / cytoplasmic exosome (RNase complex) / TRAMP-dependent tRNA surveillance pathway / CUT catabolic process / U4 snRNA 3'-end processing / nuclear polyadenylation-dependent rRNA catabolic process / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / : / poly(A)-dependent snoRNA 3'-end processing / exosome (RNase complex) / nuclear exosome (RNase complex) / nuclear-transcribed mRNA catabolic process, non-stop decay / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / : / rRNA catabolic process / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / histone mRNA catabolic process / nuclear mRNA surveillance / nonfunctional rRNA decay / rRNA primary transcript binding / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / RNA catabolic process / rRNA metabolic process / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA catabolic process / RNA processing / RNA endonuclease activity / mRNA processing / manganese ion binding / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / 3'-5'-RNA exonuclease activity / endonuclease activity / tRNA binding / single-stranded RNA binding / nucleotide binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / nucleus / cytoplasm
Similarity search - Function
OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #880 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #700 / : / Exosome complex component CSL4, N-terminal domain / Exosome complex exonuclease Rrp40, N-terminal / Exosome complex exonuclease Rrp40 N-terminal domain / Exosome complex exonuclease RRP44, S1 domain / S1 domain / RRP4, S1 domain / Exosome complex component RRP45 ...OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #880 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #700 / : / Exosome complex component CSL4, N-terminal domain / Exosome complex exonuclease Rrp40, N-terminal / Exosome complex exonuclease Rrp40 N-terminal domain / Exosome complex exonuclease RRP44, S1 domain / S1 domain / RRP4, S1 domain / Exosome complex component RRP45 / Rrp40, S1 domain / : / : / Exosome complex component RRP40, S1 domain / Exosome-associated factor Rrp6, N-terminal / Exosome complex component CSL4, C-terminal / Exosome complex component, N-terminal domain / Exosome complex component Csl4 / Exosome complex exonuclease Rrp6-like / PMC2NT (NUC016) domain / Exosome component EXOSC1/CSL4 / Exosome complex exonuclease RRP4 N-terminal region / PIN domain / : / Exosome complex RNA-binding protein 1/RRP40/RRP4 / KH domain / Rrp44-like cold shock domain / Rrp44-like cold shock domain / Dis3-like cold-shock domain 2 / Dis3-like cold-shock domain 2 (CSD2) / GHMP Kinase, N-terminal domain / Ribonuclease II/R, conserved site / Ribonuclease II family signature. / Ribonuclease II/R / RNB domain / RNB / Helicase and RNase D C-terminal / HRDC domain / HRDC domain / HRDC domain profile. / K Homology domain, type 1 / Exoribonuclease, phosphorolytic domain 2 / 3' exoribonuclease family, domain 2 / Exoribonuclease, phosphorolytic domain 1 / PNPase/RNase PH domain superfamily / Exoribonuclease, PH domain 2 superfamily / 3' exoribonuclease family, domain 1 / HRDC domain superfamily / 3'-5' exonuclease / Large family of predicted nucleotide-binding domains / PIN domain / K Homology domain, type 1 / 3'-5' exonuclease / 3'-5' exonuclease domain / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Ribosomal Protein S8; Chain: A, domain 1 / K Homology domain, type 1 superfamily / PIN-like domain superfamily / Ribosomal Protein S5; domain 2 / S1 domain profile. / HRDC-like superfamily / Ribosomal protein S1-like RNA-binding domain / S1 domain / Nucleic acid-binding proteins / Ribonuclease H-like superfamily/Ribonuclease H / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Nucleotidyltransferase; domain 5 / Ribonuclease H superfamily / Ribonuclease H-like superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold / Beta Barrel / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
RNA / RNA (> 10) / Exosome complex component RRP43 / Exosome complex component RRP4 / Exosome complex component SKI6 / Exosome complex component MTR3 / Exosome complex component RRP46 / Exosome complex component CSL4 / Exosome complex component RRP45 / Exosome complex exonuclease DIS3 ...RNA / RNA (> 10) / Exosome complex component RRP43 / Exosome complex component RRP4 / Exosome complex component SKI6 / Exosome complex component MTR3 / Exosome complex component RRP46 / Exosome complex component CSL4 / Exosome complex component RRP45 / Exosome complex exonuclease DIS3 / Exosome complex component RRP40 / Exosome complex exonuclease RRP6 / Exosome complex component RRP42
Similarity search - Component
Biological speciesSaccharomyces cerevisiae S288c (yeast)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.812 Å
AuthorsMakino, D.L. / Conti, E.
Funding support Germany, 5items
OrganizationGrant numberCountry
Max Planck Society Germany
European CommissionERC 294371 Germany
European CommissionMarie Curie ITN RNPnet Germany
German Research FoundationSFB646, SFB1035, GRK1721, FOR1680, CIPSM Germany
Louis Jeantet Germany
CitationJournal: Nature / Year: 2015
Title: RNA degradation paths in a 12-subunit nuclear exosome complex.
Authors: Makino, D.L. / Schuch, B. / Stegmann, E. / Baumgartner, M. / Basquin, C. / Conti, E.
History
DepositionJun 12, 2015Deposition site: RCSB / Processing site: PDBE
Revision 1.0Aug 5, 2015Provider: repository / Type: Initial release
Revision 1.1Aug 12, 2015Group: Database references
Revision 1.2Sep 6, 2017Group: Advisory / Author supporting evidence / Derived calculations
Category: pdbx_audit_support / pdbx_validate_symm_contact ...pdbx_audit_support / pdbx_validate_symm_contact / struct_conn / struct_conn_type
Item: _pdbx_audit_support.funding_organization
Revision 1.3Feb 20, 2019Group: Advisory / Data collection / Derived calculations
Category: pdbx_data_processing_status / pdbx_struct_ref_seq_depositor_info ...pdbx_data_processing_status / pdbx_struct_ref_seq_depositor_info / pdbx_validate_symm_contact / struct_conn / struct_conn_type
Item: _pdbx_struct_ref_seq_depositor_info.db_seq_one_letter_code
Revision 1.4Jan 10, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Exosome complex component RRP45
B: Exosome complex component SKI6
C: Exosome complex component RRP43
D: Exosome complex component RRP46
E: Exosome complex component RRP42
F: Exosome complex component MTR3
G: Exosome complex component RRP40
H: Exosome complex component RRP4
I: Exosome complex component CSL4
J: Exosome complex exonuclease DIS3
K: Exosome complex exonuclease RRP6
R: RNA synthetic
hetero molecules


Theoretical massNumber of molelcules
Total (without water)495,92113
Polymers495,85612
Non-polymers651
Water0
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area50820 Å2
ΔGint-259 kcal/mol
Surface area157830 Å2
MethodPISA
Unit cell
Length a, b, c (Å)106.150, 177.390, 299.900
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

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Exosome complex component ... , 9 types, 9 molecules ABCDEFGHI

#1: Protein Exosome complex component RRP45 / / Ribosomal RNA-processing protein 45


Mass: 34001.859 Da / Num. of mol.: 1 / Fragment: Exosome complex component RRP45
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: RRP45, YDR280W, D9954.1 / Plasmid: pETMCN / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: Q05636
#2: Protein Exosome complex component SKI6 / / Extracellular mutant protein 20 / Ribosomal RNA-processing protein 41 / Superkiller protein 6


Mass: 27794.926 Da / Num. of mol.: 1 / Fragment: Exosome complex component RRP41
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: SKI6, ECM20, RRP41, YGR195W, G7587 / Plasmid: pETMCN / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P46948
#3: Protein Exosome complex component RRP43 / / Ribosomal RNA-processing protein 43


Mass: 44109.000 Da / Num. of mol.: 1 / Fragment: Exosome complex component RRP43
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: RRP43, YCR035C, YCR35C, YCR522 / Plasmid: pETMCN / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P25359
#4: Protein Exosome complex component RRP46 / / Ribosomal RNA-processing protein 46


Mass: 26913.988 Da / Num. of mol.: 1 / Fragment: Exosome complex component RRP46
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: RRP46, YGR095C / Plasmid: pETMCN / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P53256
#5: Protein Exosome complex component RRP42 / / Ribosomal RNA-processing protein 42


Mass: 29294.398 Da / Num. of mol.: 1 / Fragment: Exosome complex component RRP42 / Mutation: V138I
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: RRP42, YDL111C / Plasmid: pETMCN / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: Q12277
#6: Protein Exosome complex component MTR3 / / mRNA transport regulator 3


Mass: 27559.869 Da / Num. of mol.: 1 / Fragment: Exosome complex component MTR3 / Mutation: T75S, M161T
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: MTR3, YGR158C, G6676 / Plasmid: pETMCN / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P48240
#7: Protein Exosome complex component RRP40 / / Ribosomal RNA-processing protein 40


Mass: 26875.668 Da / Num. of mol.: 1 / Fragment: Exosome complex component RRP40
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: RRP40, YOL142W / Plasmid: pETMCN / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: Q08285
#8: Protein Exosome complex component RRP4 / / Ribosomal RNA-processing protein 4


Mass: 39714.445 Da / Num. of mol.: 1 / Fragment: Exosome complex component RRP4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: RRP4, YHR069C / Plasmid: pECK / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P38792
#9: Protein Exosome complex component CSL4 / / CEP1 synthetic lethal protein 4


Mass: 31911.594 Da / Num. of mol.: 1 / Fragment: Exosome complex component CSL4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: CSL4, SKI4, YNL232W, N1154 / Plasmid: pETMCN-CDF / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P53859

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Exosome complex exonuclease ... , 2 types, 2 molecules JK

#10: Protein Exosome complex exonuclease DIS3 / Chromosome disjunction protein 3 / Ribosomal RNA-processing protein 44


Mass: 113983.898 Da / Num. of mol.: 1 / Fragment: Exosome complex exonuclease RRP44 / Mutation: D171N, D551N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: DIS3, RRP44, YOL021C, O2197 / Plasmid: pETM11 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS
References: UniProt: Q08162, Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters, Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters
#11: Protein Exosome complex exonuclease RRP6 / Ribosomal RNA-processing protein 6


Mass: 79649.438 Da / Num. of mol.: 1 / Fragment: Exosome complex exonuclease RRP6 / Mutation: D296N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Gene: RRP6, UNC733, YOR001W / Plasmid: pETM11 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS
References: UniProt: Q12149, Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters

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RNA chain / Non-polymers , 2 types, 2 molecules R

#12: RNA chain RNA synthetic


Mass: 14046.938 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#13: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 5

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Sample preparation

CrystalDensity Matthews: 2.85 Å3/Da / Density % sol: 56.8 % / Description: long needles
Crystal growTemperature: 283 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 7%(w/v) PEG 8000, 0.1M MES pH 6.5, 0.15M NaCl, 5%(v/v) Glycerol, 283K or 292K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1.2398 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 8, 2014
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.2398 Å / Relative weight: 1
ReflectionResolution: 3.81→57.887 Å / Num. obs: 45806 / % possible obs: 80.8 % / Observed criterion σ(I): -3 / Redundancy: 5.6 % / Biso Wilson estimate: 159.59 Å2 / Rmerge F obs: 0.988 / Rmerge(I) obs: 0.163 / Rrim(I) all: 0.175 / Χ2: 0.992 / Net I/σ(I): 4.83 / Num. measured all: 256794
Reflection shell

Diffraction-ID: 1 / Rejects: 0

Resolution (Å)Highest resolution (Å)Redundancy (%)Rmerge F obsRmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsRrim(I) all% possible all
3.81-4.21.80.6560.8430.6999321448454991.08138
4.2-4.50.760.7231.218160775463780.86682.3
4.5-50.7330.7451.8232448916787310.86395.2
5-5.50.8180.7762.2832470616261350.86299.6
5.5-60.8730.5633.1725627430742960.61899.7
6-6.20.9250.4533.998578134313410.49399.9
6.2-6.50.9440.4284.5511932173317320.46499.9
6.5-6.80.9620.3555.2410364144114410.382100
6.8-70.9680.3236.3371588378370.343100
7-7.20.9670.3296.5563177487480.35100
7.2-7.50.9710.2647.886209779770.281100
7.5-7.80.9860.2359.4986838208200.247100
7.8-80.9870.2149.9650165025020.225100
8-90.9940.15713.8321517185818580.164100
9-100.9950.12418.1114164118911890.13100
10-200.9950.10120.1531389288328810.10699.9
200.9790.09121.744194664410.09894.6

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Processing

Software
NameVersionClassification
XDSdata reduction
XSCALEdata scaling
PHASERphasing
PHENIXrefinement
Cootmodel building
PDB_EXTRACT3.15data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4IFD, 2HBJ

4ifd

2hbj


Resolution: 3.812→57.887 Å / SU ML: 0.63 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 39.7 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2996 2286 5 %Random selection
Rwork0.2947 43437 --
obs0.2949 45723 81.45 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 458.56 Å2 / Biso mean: 270.9274 Å2 / Biso min: 157.62 Å2
Refinement stepCycle: final / Resolution: 3.812→57.887 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms27238 557 1 0 27796
Biso mean--298.46 --
Num. residues----3664
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00428314
X-RAY DIFFRACTIONf_angle_d0.70438587
X-RAY DIFFRACTIONf_chiral_restr0.0454608
X-RAY DIFFRACTIONf_plane_restr0.0054925
X-RAY DIFFRACTIONf_dihedral_angle_d10.1710402
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 16

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.8119-3.89480.4787390.452974478323
3.8948-3.98530.5143440.442181986325
3.9853-4.0850.4432840.43351591167548
4.085-4.19540.36441020.40721958206060
4.1954-4.31880.40271390.38932667280681
4.3188-4.45820.41171440.35412718286282
4.4582-4.61740.371590.32893021318091
4.6174-4.80220.34461670.33653167333496
4.8022-5.02070.35971640.32393126329095
5.0207-5.28520.37371750.3363313348899
5.2852-5.61610.32681750.322633263501100
5.6161-6.04930.32541740.343632973471100
6.0493-6.65720.32071760.326433593535100
6.6572-7.61870.31461770.304533673544100
7.6187-9.59170.24531800.240134093589100
9.5917-57.89390.23081870.241835553742100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.88280.9275-1.33983.7621-1.58573.00690.0172-0.33140.4191-0.0973-0.035-0.5927-0.9580.2048-02.3625-0.0613-0.0591.9066-0.0121.956137.1508-10.0201-23.5391
25.5065-0.026-1.26074.0427-2.4228.74030.04791.1699-0.17120.18190.1097-0.5656-0.97241.37701.8146-0.1073-0.05842.8185-0.1082.079156.9644-30.5333-37.852
34.9594-2.55030.71785.205-0.6656.4331-0.24021.8740.1253-1.3008-0.3529-0.4626-0.0160.52802.1776-0.12740.05282.88650.01272.050516.048-39.1579-68.5569
40.6142-0.3416-0.75740.59911.08064.11530.29910.7874-0.75580.1233-0.17860.90251.2757-0.908302.1531-0.0247-0.10192.28660.04562.176834.2397-51.5699-12.984
50.0313-0.0336-0.15993.6037-0.8960.4070.43570.66830.67121.0773-0.81141.08210.9081-1.018402.1771-0.2324-0.09552.08110.12752.413-2.5277-29.0625-5.1192
61.44920.989-1.03862.34231.58362.4184-0.16450.1293-2.07241.2091-0.6527-0.25171.65520.7594-02.51860.1445-0.13962.453-0.34422.897562.7386-51.8998-23.0794
72.7509-0.7895-2.18621.880.38890.7755-0.86052.1228-2.6217-1.73110.3504-1.0993-0.3655-1.5651-0.00813.57-0.5858-0.03683.9445-0.48042.5125.0422-67.615-70.5415
83.2807-0.34050.7957-0.41520.54721.4906-0.5488-0.63481.01060.40740.36570.3811-1.5924-1.150804.33850.55650.01982.77240.46973.4507-5.309123.5888-31.6154
94.7383-0.70760.94142.43142.2543-2.76680.0486-1.1903-1.4095-2.89620.3893-2.2604-0.41630.53740.19046.1937-0.58020.47612.96030.27265.490340.832134.0273-21.4489
102.30870.51180.01173.13052.83353.5138-0.40440.44760.2640.8130.3492-0.2472-1.11961.1443-02.1993-0.1639-0.25922.59530.47212.418247.0523-34.2815-0.8739
11-0.1945-0.4259-0.31440.14840.2211-0.2263-0.6274-1.1654-0.6398-0.15850.4662-0.46920.3390.764203.76610.39670.04713.44820.2542.733324.1548-23.6501-39.1507
120.7747-1.1372-1.2121.58531.58181.48250.4133-0.1714-0.19863.2868-0.848-1.61590.04420.783-03.76910.1326-0.63532.9839-0.03813.812711.8988-63.2344-12.8075
134.0612-1.13111.38276.00110.06440.36131.2036-0.2949-0.9548-0.8512-1.1567-1.0282-0.10590.8414-0.00174.80420.2071-0.18044.4657-0.11614.291371.6626-86.7594-33.168
14-0.12810.10670.01782.1407-2.7532.05050.694-0.02983.28871.44460.30261.2495-1.72022.2130.01123.80380.05230.46613.16160.60964.877722.923425.9539-62.6001
151.8688-0.0919-1.95610.39270.53872.67230.2263-0.4319-1.3091.1190.69531.4877-1.08420.51380.00525.7839-0.51420.48822.12310.55864.326211.453347.1792-44.2613
16-0.0703-0.0305-0.2766-0.3289-0.1869-0.00684.55630.12880.9445-4.711-0.7431-0.8154-0.6349-0.43321.42117.7025-0.893-0.58534.84011.2945.426141.935358.6673-50.8237
176.51760.20772.7653.0372-1.79146.2071-0.58750.56230.696-0.02940.89970.5732-1.0453-0.7075-0.00332.08780.1938-0.07851.76590.40772.13988.1136-13.179-24.8089
181.89431.18540.30394.0315-0.52042.8471-0.05710.66030.2234-0.9195-0.0153-0.4625-0.1670.7877-02.2507-0.28280.25953.0192-0.04752.050745.4491-28.4707-63.2161
192.344-0.8709-0.62868.9652-0.71664.0346-0.63220.67980.5413-0.24050.47490.3588-0.4108-1.3778-02.0323-0.0239-0.36042.50410.39732.4007-2.1801-22.0168-54.6603
202.81620.9783-3.33012.3082-0.73174.4373-0.60530.2164-0.93120.17580.074-0.39641.59191.012302.3147-0.0689-0.11472.4070.08292.68293.7312-55.8822-28.5757
216.6096-1.1081.75716.7667-0.07798.3395-0.39910.02620.0970.01880.08450.30490.7915-1.3175-01.7613-0.23170.0392.5172-0.06492.345-17.4942-46.3078-34.7884
225.18130.4803-0.28140.0627-0.55680.00491.6433-2.0959-4.418-1.4868-1.3786-1.14821.68050.34160.04472.7845-0.38050.80092.8044-0.60372.24244.6456-44.8341-76.3704
230.077-0.56960.48060.2847-0.23180.310.4975-0.2453-0.2374-0.72890.47161.99542.1631-1.826703.59070.3511-0.44953.8608-0.89943.772448.3783-67.2796-62.2859
245.4227-1.07143.54040.7682-0.67274.9791-0.5225-0.2474-1.51640.15610.4351-0.38230.4099-0.279502.36660.3-0.14342.4991-0.07592.923433.0218-63.4348-40.7112
Refinement TLS group
IDRefine-IDRefine TLS-IDSelectionSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'I' and ((resseq 124:291))CHAIN A
2X-RAY DIFFRACTION2chain DD1 - 223
3X-RAY DIFFRACTION3chain F and not (resid 5:23)F4 - 5
4X-RAY DIFFRACTION3chain F and not (resid 5:23)F23 - 248
5X-RAY DIFFRACTION4chain G and resid 62:150G62 - 150
6X-RAY DIFFRACTION5chain H and resid 50:102H50 - 102
7X-RAY DIFFRACTION6chain G and resid 1:61G1 - 61
8X-RAY DIFFRACTION7(chain K and resid 532:562) or (chain I and resid 1:114)K532 - 562
9X-RAY DIFFRACTION7(chain K and resid 532:562) or (chain I and resid 1:114)I1 - 114
10X-RAY DIFFRACTION8(CHAIN J AND RESID 9:238) OR (CHAIN J AND RESID 1101)J9 - 238
11X-RAY DIFFRACTION8(chain J and resid 9:238) or (chain J and resid 1101)J1101
12X-RAY DIFFRACTION9(chain J and resid 491:909) or (chain R and resid -6:-1)J491 - 909
13X-RAY DIFFRACTION9(chain J and resid 491:909) or (chain R and resid -6:-1)R-6 - -1
14X-RAY DIFFRACTION10chain 'G' and ((resseq 151:238))G0
15X-RAY DIFFRACTION11chain 'R' and ((resseq -30:-20))R0
16X-RAY DIFFRACTION12chain 'R' and resid -44:-31R-44 - -31
17X-RAY DIFFRACTION13CHAIN K AND RESID 148:417k0
18X-RAY DIFFRACTION14chain 'J' and ((resseq 252:398))J0
19X-RAY DIFFRACTION15chain 'J' and ((resseq 399:490))J0
20X-RAY DIFFRACTION16(chain J and resid 910:1001)J910 - 1001
21X-RAY DIFFRACTION17chain BB1 - 244
22X-RAY DIFFRACTION18chain C or (chain F and resid 5:20)C0
23X-RAY DIFFRACTION19chain E or (chain H and resid 2:17)E0
24X-RAY DIFFRACTION20chain H and resid 103:195H103 - 195
25X-RAY DIFFRACTION21chain 'H' and ((resseq 196:356))H0
26X-RAY DIFFRACTION22chain K and resid 566:596K566 - 596
27X-RAY DIFFRACTION23chain 'K' and ((resseq 597:620))K0
28X-RAY DIFFRACTION24chain 'I' and ((resseq 124:291))I0

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