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Yorodumi- PDB-5b36: Crystal Structure of the O-Phosphoserine Sulfhydrylase from Aerop... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5b36 | ||||||
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Title | Crystal Structure of the O-Phosphoserine Sulfhydrylase from Aeropyrum pernix Complexed with Cysteine | ||||||
Components | Protein CysO | ||||||
Keywords | TRANSFERASE / Cysteine Biosynthesis / sulfhydrylase / complex with L-cysteine | ||||||
Function / homology | Function and homology information O-phosphoserine sulfhydrylase / O-phosphoserine sulfhydrylase activity / cystathionine beta-synthase / cystathionine beta-synthase activity / cysteine synthase / cysteine synthase activity / cysteine biosynthetic process from serine Similarity search - Function | ||||||
Biological species | Aeropyrum pernix K1 (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.15 Å | ||||||
Authors | Nakamura, T. / Takeda, E. / Kawai, Y. / Kataoka, M. / Ishikawa, K. | ||||||
Citation | Journal: Extremophiles / Year: 2016 Title: Role of F225 in O-phosphoserine sulfhydrylase from Aeropyrum pernix K1 Authors: Takeda, E. / Kunimoto, K. / Kawai, Y. / Kataoka, M. / Ishikawa, K. / Nakamura, T. #1: Journal: J.Mol.Biol. / Year: 2012 Title: Structural analysis of the substrate recognition mechanism in O-phosphoserine sulfhydrylase from the hyperthermophilic archaeon Aeropyrum pernix K1 Authors: Nakamura, T. / Kawai, Y. / Kunimoto, K. / Iwasaki, Y. / Nishii, K. / Kataoka, M. / Ishikawa, K. #2: Journal: J.Mol.Biol. / Year: 2005 Title: Three-dimensional structure of a new enzyme, O-phosphoserine sulfhydrylase, involved in l-cysteine biosynthesis by a hyperthermophilic archaeon, Aeropyrum pernix K1, at 2.0A resolution Authors: Oda, Y. / Mino, K. / Ishikawa, K. / Ataka, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5b36.cif.gz | 165.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5b36.ent.gz | 130.3 KB | Display | PDB format |
PDBx/mmJSON format | 5b36.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b3/5b36 ftp://data.pdbj.org/pub/pdb/validation_reports/b3/5b36 | HTTPS FTP |
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-Related structure data
Related structure data | 5b3aC 3vsaS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 42025.855 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aeropyrum pernix K1 (archaea) / Strain: K1 / Gene: cysO, APE_1586 / Plasmid: pET3d / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta (DE3) References: UniProt: Q9YBL2, cystathionine beta-synthase, cysteine synthase, O-phosphoserine sulfhydrylase #2: Chemical | #3: Chemical | #4: Chemical | ChemComp-MPD / ( | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.24 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1 M HEPES sodium pH 7.5, 27% 2-propanol, 10% PEG4000, 12 mM TCEP-HCl, the crystal was soaked with the reservoir solution containing 6% MPD as a cryoprotectant, 20 mM L-cysteine, and 12 mM TCEP-HCl |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å |
Detector | Type: Bruker DIP-6040 / Detector: CCD / Date: Jan 29, 2010 |
Radiation | Monochromator: Rotated-inclined double-crystal monochromator , Si (111) Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→49.1 Å / Num. obs: 43621 / % possible obs: 92 % / Redundancy: 13.5 % / Net I/σ(I): 17 |
Reflection shell | Resolution: 2.14→2.18 Å |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: CysO free form (PDB ID, 3VSA) Resolution: 2.15→49.1 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.935 / SU B: 5.615 / SU ML: 0.145 / Cross valid method: THROUGHOUT / ESU R: 0.279 / ESU R Free: 0.224 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.235 Å2
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Refinement step | Cycle: 1 / Resolution: 2.15→49.1 Å
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