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基本情報
登録情報 | データベース: PDB / ID: 5aiy | ||||||
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タイトル | R6 HUMAN INSULIN HEXAMER (SYMMETRIC), NMR, 'RED' SUBSTATE, AVERAGE STRUCTURE | ||||||
![]() | (PROTEIN (INSULIN)) x 2 | ||||||
![]() | HORMONE/GROWTH FACTOR / HORMONE / GLUCOSE METABOLISM / HORMONE-GROWTH FACTOR COMPLEX | ||||||
機能・相同性 | ![]() negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of respiratory burst / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / negative regulation of acute inflammatory response / alpha-beta T cell activation / negative regulation of respiratory burst involved in inflammatory response / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of protein secretion / regulation of protein localization to plasma membrane / fatty acid homeostasis / Signal attenuation / negative regulation of lipid catabolic process / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / COPI-mediated anterograde transport / positive regulation of lipid biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / positive regulation of insulin receptor signaling pathway / nitric oxide-cGMP-mediated signaling / transport vesicle / positive regulation of protein autophosphorylation / Insulin receptor recycling / neuron projection maintenance / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / positive regulation of brown fat cell differentiation / positive regulation of glycolytic process / activation of protein kinase B activity / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of nitric-oxide synthase activity / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation / acute-phase response / Regulation of insulin secretion / endosome lumen / positive regulation of protein secretion / positive regulation of glucose import / negative regulation of proteolysis / positive regulation of cell differentiation / regulation of transmembrane transporter activity / insulin-like growth factor receptor binding / wound healing / insulin receptor binding / regulation of synaptic plasticity / negative regulation of protein catabolic process / hormone activity / cognition / positive regulation of neuron projection development / positive regulation of protein localization to nucleus / Golgi lumen / vasodilation / glucose metabolic process / regulation of protein localization / insulin receptor signaling pathway / cell-cell signaling / glucose homeostasis / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / endoplasmic reticulum lumen / Golgi membrane / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / SYMMETRY-ADR METHOD: SIMULATED ANNEALING, WATER-SHELL REFINEMENT, AND ENERGY MINIMIZATION | ||||||
![]() | O'Donoghue, S.I. / Chang, X. / Abseher, R. / Nilges, M. / Led, J.J. | ||||||
![]() | ![]() タイトル: Unraveling the symmetry ambiguity in a hexamer: calculation of the R6 human insulin structure. 著者: O'Donoghue, S.I. / Chang, X. / Abseher, R. / Nilges, M. / Led, J.J. #1: ![]() 年: 1999 タイトル: Calculation of Symmetric Oligomer Structures from NMR Data 著者: O'Donoghue, S.I. / Nilges, M. #2: ![]() タイトル: Solution Structures of the R6 Human Insulin Hexamer 著者: Chang, X. / Jorgensen, A.M. / Bardrum, P. / Led, J.J. | ||||||
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-検証レポート
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 6 / 断片: ALPHA CHAIN / 由来タイプ: 合成 詳細: THE PROTEIN WAS CHEMICALLY SYNTHESIZED FROM THE SECRETION OF HUMAN (HOMO SAPIENS) PANCREATIC CELLS. 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 6 / 断片: BETA CHAIN / 由来タイプ: 合成 詳細: THE PROTEIN WAS CHEMICALLY SYNTHESIZED FROM THE SECRETION OF HUMAN (HOMO SAPIENS) PANCREATIC CELLS. 参照: UniProt: P01308 #3: 化合物 | ChemComp-IPH / |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||
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NMR実験 |
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試料調製
詳細 | 内容: H2O AND D2O |
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試料状態 | pH: 8.0 / 圧: 1 atm / 温度: 310 K |
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター |
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解析
NMR software |
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精密化 | 手法: SYMMETRY-ADR METHOD: SIMULATED ANNEALING, WATER-SHELL REFINEMENT, AND ENERGY MINIMIZATION ソフトェア番号: 1 詳細: REFINEMENT WAS DONE IN A SHELL OF WATER MOLECULES (J.P.LINGE,M.NILGES,1998, J.BIOMOL.NMR, IN PRESS). | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: LOWEST TOTAL ENERGY / 計算したコンフォーマーの数: 40 / 登録したコンフォーマーの数: 1 |