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Open data
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Basic information
Entry | Database: PDB / ID: 5a0o | ||||||
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Title | adhiron raised against p300 | ||||||
![]() | ADHIRON | ||||||
![]() | DE NOVO PROTEIN / AFFIMER | ||||||
Function / homology | Nuclear Transport Factor 2; Chain: A, - #10 / Nuclear Transport Factor 2; Chain: A, / Roll / Alpha Beta![]() | ||||||
Biological species | SYNTHETIC CONSTRUCT (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kyle, H.F. / Wickson, K.F. / Stott, J. / Burslem, G.M. / Breeze, A.L. / Tiede, C. / Tomlinson, D.C. / Warriner, S.L. / Nelson, A. / Wilson, A.J. / Edwards, T.A. | ||||||
![]() | ![]() Title: Exploration of the Hif-1Alpha.P300 Interface Using Peptide and Adhiron Phage Display Technologies Authors: Kyle, H.F. / Wickson, K.F. / Stott, J. / Burslem, G.M. / Breeze, A.L. / Tiede, C. / Tomlinson, D.C. / Warriner, S.L. / Nelson, A. / Wilson, A.J. / Edwards, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83.4 KB | Display | ![]() |
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PDB format | ![]() | 66 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 429 KB | Display | ![]() |
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Full document | ![]() | 435 KB | Display | |
Data in XML | ![]() | 10.1 KB | Display | |
Data in CIF | ![]() | 13 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4n6uS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 12465.246 Da / Num. of mol.: 2 / Fragment: ADHIRON Source method: isolated from a genetically manipulated source Source: (gene. exp.) SYNTHETIC CONSTRUCT (others) / Description: DESIGNED NON-ANTIBODY BINDING PROTEIN / Plasmid: PET11 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.67 Å3/Da / Density % sol: 66.5 % / Description: NONE |
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Crystal grow | pH: 7 / Details: 0.8 M DI-SODIUM SUCCINATE PH7 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS / Detector: PIXEL / Date: Feb 4, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91741 Å / Relative weight: 1 |
Reflection | Resolution: 2.73→36.73 Å / Num. obs: 7134 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 12.6 % / Rmerge(I) obs: 0.15 / Net I/σ(I): 12.3 |
Reflection shell | Resolution: 2.73→2.8 Å / Redundancy: 13.8 % / Rmerge(I) obs: 1.44 / Mean I/σ(I) obs: 1.7 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4N6U Resolution: 2.73→36.73 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.911 / SU B: 14.875 / SU ML: 0.294 / Cross valid method: THROUGHOUT / ESU R: 0.91 / ESU R Free: 0.363 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 70.371 Å2
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Refinement step | Cycle: LAST / Resolution: 2.73→36.73 Å
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Refine LS restraints |
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