+Open data
-Basic information
Entry | Database: PDB / ID: 4zdr | ||||||
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Title | Crystal structure of 14-3-3[zeta]-LKB1 fusion protein | ||||||
Components | 14-3-3 protein zeta/delta,GGSGGS linker,Serine/threonine-protein kinase STK11 | ||||||
Keywords | PROTEIN BINDING / chimeric protein / regulatory protein / tumor suppressor / serine/threonine-protein kinase | ||||||
Function / homology | Function and homology information positive regulation of vesicle transport along microtubule / intracellular protein-containing complex / Golgi localization / LRR domain binding / AMPK inhibits chREBP transcriptional activation activity / Golgi reassembly / dendrite extension / negative regulation of epithelial cell proliferation involved in prostate gland development / regulation of synapse maturation / NOTCH4 Activation and Transmission of Signal to the Nucleus ...positive regulation of vesicle transport along microtubule / intracellular protein-containing complex / Golgi localization / LRR domain binding / AMPK inhibits chREBP transcriptional activation activity / Golgi reassembly / dendrite extension / negative regulation of epithelial cell proliferation involved in prostate gland development / regulation of synapse maturation / NOTCH4 Activation and Transmission of Signal to the Nucleus / serine/threonine protein kinase complex / tissue homeostasis / establishment of Golgi localization / epithelial cell proliferation involved in prostate gland development / response to thyroid hormone / Energy dependent regulation of mTOR by LKB1-AMPK / positive thymic T cell selection / Rap1 signalling / vasculature development / G1 to G0 transition / anoikis / negative regulation of cold-induced thermogenesis / regulation of Wnt signaling pathway / negative regulation of protein localization to nucleus / response to glucagon / KSRP (KHSRP) binds and destabilizes mRNA / FOXO-mediated transcription of cell death genes / response to ionizing radiation / GP1b-IX-V activation signalling / positive regulation of axonogenesis / regulation of dendrite morphogenesis / establishment of cell polarity / cellular response to UV-B / response to lipid / protein kinase activator activity / regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of transforming growth factor beta receptor signaling pathway / activation of protein kinase activity / protein localization to nucleus / Regulation of localization of FOXO transcription factors / intrinsic apoptotic signaling pathway by p53 class mediator / Interleukin-3, Interleukin-5 and GM-CSF signaling / phosphoserine residue binding / Activation of BAD and translocation to mitochondria / cellular response to glucose starvation / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / positive regulation of autophagy / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / RHO GTPases activate PKNs / positive regulation of gluconeogenesis / negative regulation of TORC1 signaling / negative regulation of innate immune response / axonogenesis / protein dephosphorylation / regulation of ERK1 and ERK2 cascade / protein sequestering activity / response to activity / regulation of signal transduction by p53 class mediator / Translocation of SLC2A4 (GLUT4) to the plasma membrane / Deactivation of the beta-catenin transactivating complex / regulation of cell growth / TP53 Regulates Metabolic Genes / Negative regulation of NOTCH4 signaling / peptidyl-threonine phosphorylation / negative regulation of canonical Wnt signaling pathway / negative regulation of cell growth / Z disc / autophagy / positive regulation of protein localization to nucleus / melanosome / p53 binding / glucose homeostasis / T cell receptor signaling pathway / spermatogenesis / blood microparticle / DNA-binding transcription factor binding / Regulation of TP53 Activity through Phosphorylation / vesicle / transmembrane transporter binding / protein autophosphorylation / regulation of cell cycle / non-specific serine/threonine protein kinase / cadherin binding / negative regulation of cell population proliferation / protein phosphorylation / focal adhesion / protein serine kinase activity / protein serine/threonine kinase activity / glutamatergic synapse / DNA damage response / ubiquitin protein ligase binding / protein-containing complex binding / negative regulation of apoptotic process / protein kinase binding / negative regulation of transcription by RNA polymerase II / magnesium ion binding / signal transduction / mitochondrion / extracellular space Similarity search - Function | ||||||
Biological species | Homo sapiens (human) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.899 Å | ||||||
Authors | Ding, S. / Shi, Z.B. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.F / Year: 2015 Title: Structure of the 14-3-3 zeta-LKB1 fusion protein provides insight into a novel ligand-binding mode of 14-3-3. Authors: Ding, S. / Zhou, R. / Zhu, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4zdr.cif.gz | 107.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4zdr.ent.gz | 82 KB | Display | PDB format |
PDBx/mmJSON format | 4zdr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zd/4zdr ftp://data.pdbj.org/pub/pdb/validation_reports/zd/4zdr | HTTPS FTP |
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-Related structure data
Related structure data | 4fj3S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 30349.270 Da / Num. of mol.: 2 / Fragment: UNP residues 1-230,UNP residues 333-340 / Mutation: T240E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human), (gene. exp.) synthetic construct (others) Gene: YWHAZ, STK11, LKB1, PJS / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): Codonplus / References: UniProt: P63104, UniProt: Q15831 #2: Chemical | #3: Chemical | #4: Chemical | ChemComp-TME / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.9 Å3/Da / Density % sol: 68.47 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 1.4 M Li2SO4, 0.1 M BIS-TRIS propane |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.9793 Å |
Detector | Type: RAYONIX MX-225 / Detector: CCD / Date: Apr 12, 2013 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 2.899→50 Å / Num. obs: 19507 / % possible obs: 100 % / Redundancy: 10.9 % / Net I/σ(I): 43 |
Reflection shell | Resolution: 2.899→2.95 Å / Redundancy: 11.3 % / Mean I/σ(I) obs: 3.2 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4FJ3 Resolution: 2.899→47.964 Å / SU ML: 0.38 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 27.84 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.899→47.964 Å
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Refine LS restraints |
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LS refinement shell |
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