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- PDB-4ywm: Pyrococcus furiosus MCM N-terminal domain beta-turn triple mutant... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ywm | |||||||||
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Title | Pyrococcus furiosus MCM N-terminal domain beta-turn triple mutant pentameric ring | |||||||||
![]() | Cell division control protein 21 | |||||||||
![]() | CELL CYCLE / MCM / helicase / replication / OB-fold | |||||||||
Function / homology | ![]() single-stranded 3'-5' DNA helicase activity / MCM complex / intein-mediated protein splicing / double-strand break repair via break-induced replication / DNA unwinding involved in DNA replication / single-stranded DNA binding / endonuclease activity / DNA helicase / cell division / ATP binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Froelich, C.A. / Enemark, E.J. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: MCM ring hexamerization is a prerequisite for DNA-binding. Authors: Froelich, C.A. / Nourse, A. / Enemark, E.J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 451.7 KB | Display | ![]() |
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PDB format | ![]() | 382.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 515.9 KB | Display | ![]() |
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Full document | ![]() | 546.7 KB | Display | |
Data in XML | ![]() | 85.2 KB | Display | |
Data in CIF | ![]() | 111.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ywkC ![]() 4ywlC ![]() 4pofS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Beg auth comp-ID: VAL / Beg label comp-ID: VAL / End auth comp-ID: SER / End label comp-ID: SER / Refine code: 1 / Auth seq-ID: 1 - 253 / Label seq-ID: 2 - 254
NCS ensembles :
NCS oper:
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Details | The biological assembly for the wild-type protein is a hexamer. The beta turn triple mutant is a pentamer in solution by analytical ultracentrifugation. A pentameric ring flanked by 5 peripheral monomers is observed in the crystal structure. |
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Components
#1: Protein | Mass: 29198.395 Da / Num. of mol.: 10 / Mutation: K233A, R234A, K236A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-SO4 / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.6 Å3/Da / Density % sol: 73.27 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 5.5 / Details: 100 mM Bis-Tris, pH 5.5, 1.75 M ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Dec 12, 2013 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→50 Å / Num. obs: 88667 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 10.9 % / Rsym value: 0.135 / Net I/σ(I): 18 |
Reflection shell | Resolution: 3.2→3.31 Å / Redundancy: 10.1 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 3.9 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4POF Resolution: 3.2→50 Å / Cor.coef. Fo:Fc: 0.904 / Cor.coef. Fo:Fc free: 0.874 / SU B: 19.31 / SU ML: 0.315 / Cross valid method: THROUGHOUT / ESU R: 0.757 / ESU R Free: 0.439 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 68.115 Å2
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Refinement step | Cycle: 1 / Resolution: 3.2→50 Å
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Refine LS restraints |
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