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- PDB-4yee: beta2 carbohydrate binding module (CBM) of AMP-activated protein ... -

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Basic information

Entry
Database: PDB / ID: 4yee
Titlebeta2 carbohydrate binding module (CBM) of AMP-activated protein kinase (AMPK) in complex with glucosyl-beta-cyclodextrin
Components5'-AMP-activated protein kinase subunit beta-2
KeywordsSUGAR BINDING PROTEIN / carbohydrate binding module (CBM) / AMP-activated protein kinase (AMPK) / glycogen
Function / homology
Function and homology information


AMPK inhibits chREBP transcriptional activation activity / Carnitine shuttle / Energy dependent regulation of mTOR by LKB1-AMPK / Regulation of TP53 Activity through Phosphorylation / Macroautophagy / TP53 Regulates Metabolic Genes / nucleotide-activated protein kinase complex / cAMP-dependent protein kinase complex / cellular response to nutrient levels / fatty acid biosynthetic process ...AMPK inhibits chREBP transcriptional activation activity / Carnitine shuttle / Energy dependent regulation of mTOR by LKB1-AMPK / Regulation of TP53 Activity through Phosphorylation / Macroautophagy / TP53 Regulates Metabolic Genes / nucleotide-activated protein kinase complex / cAMP-dependent protein kinase complex / cellular response to nutrient levels / fatty acid biosynthetic process / positive regulation of cold-induced thermogenesis / apical plasma membrane / protein kinase binding / enzyme binding / signal transduction / nucleoplasm / nucleus / cytoplasm
Similarity search - Function
Association with the SNF1 complex (ASC) domain / ASC domain superfamily / : / 5'-AMP-activated protein kinase beta subunit, interaction domain / 5'-AMP-activated protein kinase beta subunit, interation domain / AMP-activated protein kinase, glycogen-binding domain / Glycogen recognition site of AMP-activated protein kinase / Immunoglobulin E-set / Immunoglobulin-like fold / Immunoglobulins ...Association with the SNF1 complex (ASC) domain / ASC domain superfamily / : / 5'-AMP-activated protein kinase beta subunit, interaction domain / 5'-AMP-activated protein kinase beta subunit, interation domain / AMP-activated protein kinase, glycogen-binding domain / Glycogen recognition site of AMP-activated protein kinase / Immunoglobulin E-set / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
6-O-alpha-D-Glucosyl-beta-cyclodextrin / 5'-AMP-activated protein kinase subunit beta-2
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsMobbs, J. / Gorman, M.A. / Parker, M.W. / Gooley, P.R. / Griffin, M.
CitationJournal: Biochem.J. / Year: 2015
Title: Determinants of oligosaccharide specificity of the carbohydrate-binding modules of AMP-activated protein kinase.
Authors: Mobbs, J.I. / Koay, A. / Di Paolo, A. / Bieri, M. / Petrie, E.J. / Gorman, M.A. / Doughty, L. / Parker, M.W. / Stapleton, D.I. / Griffin, M.D. / Gooley, P.R.
History
DepositionFeb 24, 2015Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 1, 2015Provider: repository / Type: Initial release
Revision 1.1Jun 3, 2015Group: Database references
Revision 2.0Jul 29, 2020Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Database references / Derived calculations / Non-polymer description / Source and taxonomy / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / citation / diffrn_source / entity / entity_src_gen / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_oper_list / pdbx_validate_close_contact / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _citation.journal_id_CSD / _diffrn_source.pdbx_synchrotron_site / _entity.formula_weight / _entity.pdbx_description / _entity.pdbx_number_of_molecules / _entity.src_method / _entity.type / _entity_src_gen.pdbx_alt_source_flag / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_oper_list.symmetry_operation / _struct_asym.entity_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 3.0Oct 14, 2020Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / pdbx_branch_scheme / pdbx_molecule_features / pdbx_validate_close_contact / struct_conn
Item: _atom_site.auth_seq_id / _chem_comp.pdbx_synonyms ..._atom_site.auth_seq_id / _chem_comp.pdbx_synonyms / _pdbx_branch_scheme.pdb_seq_num / _pdbx_validate_close_contact.auth_seq_id_1 / _pdbx_validate_close_contact.auth_seq_id_2 / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr2_auth_seq_id
Revision 3.1Sep 27, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 5'-AMP-activated protein kinase subunit beta-2
B: 5'-AMP-activated protein kinase subunit beta-2
C: 5'-AMP-activated protein kinase subunit beta-2
D: 5'-AMP-activated protein kinase subunit beta-2
E: 5'-AMP-activated protein kinase subunit beta-2
F: 5'-AMP-activated protein kinase subunit beta-2
G: 5'-AMP-activated protein kinase subunit beta-2
H: 5'-AMP-activated protein kinase subunit beta-2
I: 5'-AMP-activated protein kinase subunit beta-2
J: 5'-AMP-activated protein kinase subunit beta-2
K: 5'-AMP-activated protein kinase subunit beta-2
L: 5'-AMP-activated protein kinase subunit beta-2
M: 5'-AMP-activated protein kinase subunit beta-2
N: 5'-AMP-activated protein kinase subunit beta-2
O: 5'-AMP-activated protein kinase subunit beta-2
P: 5'-AMP-activated protein kinase subunit beta-2
Q: 5'-AMP-activated protein kinase subunit beta-2
R: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)205,82255
Polymers180,40018
Non-polymers25,42237
Water30,2111677
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A: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,5224
Polymers10,0221
Non-polymers1,4993
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,5224
Polymers10,0221
Non-polymers1,4993
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,5224
Polymers10,0221
Non-polymers1,4993
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,4293
Polymers10,0221
Non-polymers1,4072
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,4293
Polymers10,0221
Non-polymers1,4072
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,4293
Polymers10,0221
Non-polymers1,4072
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,3372
Polymers10,0221
Non-polymers1,3151
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,5224
Polymers10,0221
Non-polymers1,4993
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
9
I: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,5224
Polymers10,0221
Non-polymers1,4993
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
10
J: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,3372
Polymers10,0221
Non-polymers1,3151
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
11
K: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,4293
Polymers10,0221
Non-polymers1,4072
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
12
L: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,4293
Polymers10,0221
Non-polymers1,4072
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
13
M: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,4293
Polymers10,0221
Non-polymers1,4072
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
14
N: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,5224
Polymers10,0221
Non-polymers1,4993
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
15
O: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,3372
Polymers10,0221
Non-polymers1,3151
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
16
P: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,4293
Polymers10,0221
Non-polymers1,4072
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
17
Q: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,3372
Polymers10,0221
Non-polymers1,3151
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
18
R: 5'-AMP-activated protein kinase subunit beta-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,3372
Polymers10,0221
Non-polymers1,3151
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)204.538, 96.432, 118.724
Angle α, β, γ (deg.)90.00, 125.46, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein
5'-AMP-activated protein kinase subunit beta-2 / AMPK subunit beta-2


Mass: 10022.198 Da / Num. of mol.: 18 / Fragment: UNP residues 74-155
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Prkab2 / Cell line (production host): BL21 (DE3) / Production host: Escherichia coli (E. coli) / References: UniProt: Q9QZH4
#2: Polysaccharide
Cyclic alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D- ...Cyclic alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-[alpha-D-glucopyranose-(1-6)]alpha-D-glucopyranose


Type: oligosaccharide, Oligosaccharide / Class: Drug delivery / Mass: 1315.142 Da / Num. of mol.: 18
Source method: isolated from a genetically manipulated source
Details: cyclic oligosaccharide / References: 6-O-alpha-D-Glucosyl-beta-cyclodextrin
DescriptorTypeProgram
WURCS=2.0/1,8,8/[a2122h-1a_1-5]/1-1-1-1-1-1-1-1/a1-g4_a4-b1_b4-c1_c4-d1_d4-e1_e4-f1_f4-g1_g6-h1WURCSPDB2Glycan 1.1.0
#3: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 19 / Source method: obtained synthetically / Formula: C3H8O3
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1677 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.64 Å3/Da / Density % sol: 53.47 %
Crystal growTemperature: 281.15 K / Method: vapor diffusion, sitting drop / pH: 7
Details: in 0.2 M lithium chloride, 20% w/v PEG 6000 and 0.1 M sodium HEPES pH 7

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX1 / Wavelength: 0.9537 Å
DetectorType: ADSC QUANTUM 210r / Detector: CCD / Date: Jun 20, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9537 Å / Relative weight: 1
ReflectionResolution: 2→48.38 Å / Num. obs: 127116 / % possible obs: 99.9 % / Redundancy: 7.6 % / Rmerge(I) obs: 0.084 / Rsym value: 0.098 / Net I/σ(I): 16.9
Reflection shellResolution: 2→2.03 Å / Redundancy: 7.3 % / Rmerge(I) obs: 0.668 / Mean I/σ(I) obs: 2.8 / % possible all: 97.8

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Processing

Software
NameVersionClassification
REFMAC5.8.0073refinement
Cootmodel building
PHASERphasing
Aimlessdata scaling
XDSdata reduction
Blu-Icedata collection
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2F15

2f15


Resolution: 2→43.38 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.932 / SU B: 7.721 / SU ML: 0.124 / Cross valid method: THROUGHOUT / ESU R: 0.175 / ESU R Free: 0.163 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.22463 6385 5 %RANDOM
Rwork0.16872 ---
obs0.1715 120729 99.87 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 32.5 Å2
Baniso -1Baniso -2Baniso -3
1--0.35 Å20 Å20.2 Å2
2--0.51 Å2-0 Å2
3----0.22 Å2
Refinement stepCycle: LAST / Resolution: 2→43.38 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11966 0 1698 1677 15341
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.01914172
X-RAY DIFFRACTIONr_bond_other_d0.0010.0212478
X-RAY DIFFRACTIONr_angle_refined_deg1.682.06319551
X-RAY DIFFRACTIONr_angle_other_deg0.785329262
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.88251493
X-RAY DIFFRACTIONr_dihedral_angle_2_deg33.33624.966590
X-RAY DIFFRACTIONr_dihedral_angle_3_deg11.503151892
X-RAY DIFFRACTIONr_dihedral_angle_4_deg21.5491532
X-RAY DIFFRACTIONr_chiral_restr0.1130.22572
X-RAY DIFFRACTIONr_gen_planes_refined0.0060.02114119
X-RAY DIFFRACTIONr_gen_planes_other0.0010.022987
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it1.2811.7566008
X-RAY DIFFRACTIONr_mcbond_other1.2811.7566007
X-RAY DIFFRACTIONr_mcangle_it2.0962.6197480
X-RAY DIFFRACTIONr_mcangle_other2.0962.627481
X-RAY DIFFRACTIONr_scbond_it1.972.3718163
X-RAY DIFFRACTIONr_scbond_other1.972.3728164
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other3.0173.49812069
X-RAY DIFFRACTIONr_long_range_B_refined7.49418.09416025
X-RAY DIFFRACTIONr_long_range_B_other7.49318.09516026
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 1.998→2.05 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.275 486 -
Rwork0.237 8833 -
obs--98.49 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.84220.36370.17932.32070.18641.0063-0.12730.03970.008-0.04690.0044-0.0820.0065-0.0030.12290.0730.0059-0.05020.03340.00290.136546.5913-14.19728.354
21.504-0.54110.38281.14420.34492.10250.0183-0.0494-0.0496-0.00510.04350.04770.1589-0.0138-0.06180.10550.0325-0.03460.06590.03240.062280.7416-10.6268-7.7423
31.06320.26080.2191.67340.00470.8024-0.0148-0.0623-0.0423-0.090.03590.0933-0.0397-0.0123-0.02120.10110.0129-0.0240.0276-0.00060.123255.03419.78037.0712
40.69890.25420.12271.32340.35872.06270.0641-0.08880.04230.0091-0.0051-0.0079-0.0704-0.0539-0.0590.06570.0304-0.03110.1076-0.01630.067283.4867-32.022338.6194
51.6695-0.4816-0.3790.6639-0.18751.30180.0186-0.14990.05960.00380.0279-0.00440.04890.1264-0.04640.050.0073-0.02960.1069-0.01080.088471.72990.322324.1846
61.6747-0.3415-1.21271.56970.13211.33430.1346-0.09670.2506-0.09490.0804-0.0327-0.1140.1696-0.2150.0976-0.0142-0.00510.0461-0.04650.130685.692214.7243-9.1694
72.7216-1.0169-0.22761.85-0.0330.6447-0.0044-0.03180.11450.06080.0196-0.0292-0.03-0.1492-0.01520.02990.0444-0.05010.0948-0.05380.207228.4516-3.078322.2418
81.8693-1.2834-0.72281.99870.74612.5094-0.11680.2302-0.02450.0701-0.0964-0.07930.1937-0.25090.21320.03630.0051-0.00920.1086-0.0770.085383.6766-15.6799-39.3329
91.8362-0.353-0.98711.55430.42941.70090.2409-0.26410.23710.0982-0.1030.1021-0.04860.3062-0.13790.0973-0.04380.02270.1439-0.10760.105554.032111.086237.1028
102.0227-0.1378-1.39990.40540.2561.57290.08760.29660.213-0.00260.1051-0.0855-0.0514-0.4339-0.19270.04950.0215-0.01120.24280.04690.1364101.34023.351521.9451
113.66180.8670.42991.05260.38371.71850.0032-0.5057-0.2055-0.0894-0.2161-0.0218-0.1326-0.31150.21290.0551-0.028-0.04430.27950.02780.116265.1923-48.987931.3705
122.9072-0.26430.2631.0084-0.12441.59570.02070.33110.04610.17620.0065-0.071-0.0872-0.4191-0.02730.09020.0344-0.04840.26720.03660.102664.8037-4.0819-26.1552
130.61210.48190.10171.0219-0.46112.14030.0648-0.14830.06310.0304-0.09950.19450.01270.05080.03470.08450.0141-0.01330.0761-0.00490.12245.2042-12.542838.459
141.1514-0.31280.661.4248-1.08944.67530.15530.0403-0.05970.07660.0892-0.0346-0.2366-0.3758-0.24450.13530.06450.00370.0930.06320.131485.5293-30.075213.3252
152.3564-0.5504-0.37331.80280.24360.9418-0.0325-0.0364-0.0794-0.08990.0814-0.15810.06250.0867-0.0490.05610.0551-0.01770.0931-0.00430.104102.5762-46.560419.2985
161.4013-1.33-0.93972.22120.63621.7155-0.0265-0.1319-0.13470.09160.09370.1803-0.21390.0691-0.06720.0924-0.03690.0440.0902-0.05020.1344116.91768.795740.9917
173.00291.0093-0.79030.92930.64623.0743-0.2145-0.2012-0.4781-0.0797-0.0597-0.17120.128-0.02060.27410.1041-0.00250.00070.02510.04520.174384.441632.981513.6761
181.8402-1.3641-0.191.4210.85253.2613-0.42210.0169-0.0240.37750.2310.28270.57920.23480.19110.2992-0.00190.01430.33730.32120.369583.706131.493838.5789
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A75 - 156
2X-RAY DIFFRACTION2B74 - 156
3X-RAY DIFFRACTION3C74 - 156
4X-RAY DIFFRACTION4D78 - 156
5X-RAY DIFFRACTION5E72 - 156
6X-RAY DIFFRACTION6F72 - 156
7X-RAY DIFFRACTION7G74 - 156
8X-RAY DIFFRACTION8H72 - 156
9X-RAY DIFFRACTION9I72 - 156
10X-RAY DIFFRACTION10J72 - 156
11X-RAY DIFFRACTION11K75 - 156
12X-RAY DIFFRACTION12L75 - 156
13X-RAY DIFFRACTION13M72 - 156
14X-RAY DIFFRACTION14N73 - 156
15X-RAY DIFFRACTION15O73 - 156
16X-RAY DIFFRACTION16P72 - 156
17X-RAY DIFFRACTION17Q73 - 156
18X-RAY DIFFRACTION18R73 - 156

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