nitric oxide reductase (cytochrome c) / nitric oxide reductase activity / cytochrome-c oxidase activity / : / aerobic respiration / membrane => GO:0016020 / electron transfer activity / heme binding / metal ion binding Similarity search - Function
Cytochrome C Oxidase; Chain A / Cytochrome c oxidase-like, subunit I domain / Cytochrome c oxidase, subunit I, copper-binding site / Heme-copper oxidase catalytic subunit, copper B binding region signature. / Cytochrome c oxidase-like, subunit I domain / Cytochrome oxidase subunit I profile. / Cytochrome c oxidase subunit I / Cytochrome c oxidase-like, subunit I superfamily / Cytochrome C and Quinol oxidase polypeptide I / Cytochrome c ...Cytochrome C Oxidase; Chain A / Cytochrome c oxidase-like, subunit I domain / Cytochrome c oxidase, subunit I, copper-binding site / Heme-copper oxidase catalytic subunit, copper B binding region signature. / Cytochrome c oxidase-like, subunit I domain / Cytochrome oxidase subunit I profile. / Cytochrome c oxidase subunit I / Cytochrome c oxidase-like, subunit I superfamily / Cytochrome C and Quinol oxidase polypeptide I / Cytochrome c / Cytochrome c-like domain / Cytochrome Bc1 Complex; Chain D, domain 2 / Cytochrome c family profile. / Cytochrome c-like domain / Cytochrome c-like domain superfamily / Up-down Bundle / Orthogonal Bundle / Mainly Alpha Similarity search - Domain/homology
COPPER (II) ION / : / HEME C / PROTOPORPHYRIN IX CONTAINING FE / Unknown ligand / NorC-like protein / Nitric-oxide reductase subunit B Similarity search - Component
Mass: 18.015 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 3.96 Å3/Da / Density % sol: 68.91 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.6 Details: Crystallization reagent composed of 100 mM MOPS pH 7.6, 28 % (w/v) PEG 600. Protein solution 8mg/ml 20 mM MOPS pH 7.2, 100 mM NaCl, 0.4 % (w/v) nonyl-B-D-glucopyranoside. Mixed 1:1 in sitting drop at 4C.
Resolution: 2.85→53.36 Å / Cor.coef. Fo:Fc: 0.933 / Cor.coef. Fo:Fc free: 0.875 / SU B: 13.74 / SU ML: 0.254 / Cross valid method: THROUGHOUT / ESU R: 0.58 / ESU R Free: 0.322 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.24348
1285
5.1 %
RANDOM
Rwork
0.18848
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obs
0.19135
23917
97.1 %
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Solvent computation
Ion probe radii: 0.85 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK