cellulose 1,4-beta-cellobiosidase activity / Hydrolases; Glycosylases; Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds / cellulose binding / cellulose catabolic process / extracellular region Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 820 / Source method: isolated from a natural source / Formula: H2O
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION
-
Sample preparation
Crystal
Density Matthews: 2.06 Å3/Da / Density % sol: 40.39 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4 Details: Sitting drop with 50 uL of well solution and drops containing 0.5 uL of well solution and 1.5 uL of protein solution. Well solution: 0.27 M sodium phosphate, 1.53 M potassium phosphate ...Details: Sitting drop with 50 uL of well solution and drops containing 0.5 uL of well solution and 1.5 uL of protein solution. Well solution: 0.27 M sodium phosphate, 1.53 M potassium phosphate dibasic pH 4.0; Protein solution: 15.5 mg/ml of protein in 20 mM acetate, pH 5.0, 100 mM NaCl, 50 mM cellobiose, 5 mM cellohexaose
-
Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: ROTATING ANODE / Type: BRUKER AXS MICROSTAR / Wavelength: 1.54188 Å
Detector
Type: Bruker Platinum 135 / Detector: CCD / Date: Oct 15, 2013
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.54188 Å / Relative weight: 1
Reflection
Resolution: 1.7→84.84 Å / Num. obs: 40134 / % possible obs: 99 % / Redundancy: 8.63 % / Rmerge(I) obs: 0.1026 / Net I/σ(I): 12.99
Reflection shell
Resolution: 1.7→1.8 Å / Redundancy: 2.13 % / Rmerge(I) obs: 0.399 / Mean I/σ(I) obs: 1.66 / % possible all: 94.4
Resolution: 1.7→50 Å / Cor.coef. Fo:Fc: 0.97 / Cor.coef. Fo:Fc free: 0.925 / SU B: 2.855 / SU ML: 0.089 / Cross valid method: THROUGHOUT / ESU R: 0.111 / ESU R Free: 0.12 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.20301
1188
3.1 %
RANDOM
Rwork
0.13756
-
-
-
obs
0.13954
37520
95.51 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK