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Open data
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Basic information
Entry | Database: PDB / ID: 4x88 | |||||||||
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Title | E178D Selectivity filter mutant of NavMS voltage-gated pore | |||||||||
![]() | Ion transport protein | |||||||||
![]() | TRANSPORT PROTEIN / SELECTIVITY FILTER / MEMBRANE PROTEIN | |||||||||
Function / homology | ![]() voltage-gated sodium channel complex / membrane depolarization during action potential / voltage-gated sodium channel activity Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Naylor, C.E. / Bagneris, C. / Wallace, B.A. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular basis of ion permeability in a voltage-gated sodium channel. Authors: Naylor, C.E. / Bagneris, C. / DeCaen, P.G. / Sula, A. / Scaglione, A. / Clapham, D.E. / Wallace, B.A. #1: ![]() Title: Role of the C-terminal domain in the structure and function of tetrameric sodium channels. Authors: Bagneris, C. / Decaen, P.G. / Hall, B.A. / Naylor, C.E. / Clapham, D.E. / Kay, C.W. / Wallace, B.A. #2: ![]() Title: Structure of a bacterial voltage-gated sodium channel pore reveals mechanisms of opening and closing. Authors: McCusker, E.C. / Bagneris, C. / Naylor, C.E. / Cole, A.R. / D'Avanzo, N. / Nichols, C.G. / Wallace, B.A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 161.6 KB | Display | ![]() |
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PDB format | ![]() | 128.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1018.8 KB | Display | ![]() |
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Full document | ![]() | 1023.7 KB | Display | |
Data in XML | ![]() | 14.8 KB | Display | |
Data in CIF | ![]() | 20 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4x89C ![]() 4x8aC ![]() 5bzbC ![]() 3zjzS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 16822.477 Da / Num. of mol.: 4 Fragment: NavMS pore and C-terminal domain, UNP residues 130-274 Mutation: E178D Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-2CV / #3: Chemical | ChemComp-1PE / #4: Chemical | ChemComp-NA / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 7.63 Å3/Da / Density % sol: 83.89 % / Description: Flat plates |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 0.1 M NA3CITRATE, 0.1 M TRIS, PH 8.0, 34% PEG400 / PH range: 8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 26, 2014 |
Radiation | Monochromator: channe-cut crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.984 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→45.93 Å / Num. all: 14189 / Num. obs: 14175 / % possible obs: 99.9 % / Redundancy: 6.5 % / Biso Wilson estimate: 26 Å2 / Rmerge(I) obs: 0.251 / Net I/σ(I): 7.7 |
Reflection shell | Resolution: 3.5→3.83 Å / Redundancy: 6.5 % / Rmerge(I) obs: 0.545 / Mean I/σ(I) obs: 3.9 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3ZJZ Resolution: 3.5→45.93 Å / Cor.coef. Fo:Fc: 0.8241 / Cor.coef. Fo:Fc free: 0.8693 / SU R Cruickshank DPI: 0.799 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.774 / SU Rfree Blow DPI: 0.322 / SU Rfree Cruickshank DPI: 0.328
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Displacement parameters | Biso mean: 34.25 Å2
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Refine analyze | Luzzati coordinate error obs: 0.622 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 3.5→45.93 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.5→3.78 Å / Total num. of bins used: 7
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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