[English] 日本語
Yorodumi- PDB-4wp5: Chaetomium thermophilum Mex67 NTF2-like domain complexed with Mtr2 -
+Open data
-Basic information
Entry | Database: PDB / ID: 4wp5 | ||||||
---|---|---|---|---|---|---|---|
Title | Chaetomium thermophilum Mex67 NTF2-like domain complexed with Mtr2 | ||||||
Components |
| ||||||
Keywords | TRANSPORT PROTEIN / nuclear export factor | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.9 Å | ||||||
Authors | Aibara, S. / Valkov, E. / Stewart, M. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.F / Year: 2015 Title: Structural characterization of the principal mRNA-export factor Mex67-Mtr2 from Chaetomium thermophilum. Authors: Aibara, S. / Valkov, E. / Lamers, M.H. / Dimitrova, L. / Hurt, E. / Stewart, M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 4wp5.cif.gz | 149.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb4wp5.ent.gz | 119.3 KB | Display | PDB format |
PDBx/mmJSON format | 4wp5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wp/4wp5 ftp://data.pdbj.org/pub/pdb/validation_reports/wp/4wp5 | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-Components
#1: Protein | Mass: 23811.693 Da / Num. of mol.: 1 / Fragment: UNP residues 365-564 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0059630 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: G0SET4 |
---|---|
#2: Protein | Mass: 20923.633 Da / Num. of mol.: 1 / Fragment: UNP residues 11-187 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0065500 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: G0SG92 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 3.06 Å3/Da / Density % sol: 59.81 % |
---|---|
Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / Details: see publication for details |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.9686 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Jan 31, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9686 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→44.7 Å / Num. obs: 12265 / Redundancy: 4.3 % / Net I/σ(I): 11.4 |
-Processing
Software | Name: PHENIX / Version: (phenix.refine: 1.9_1692) / Classification: refinement | |||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.9→44.646 Å / SU ML: 0.54 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 30.05 / Stereochemistry target values: ML
| |||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→44.646 Å
| |||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||
LS refinement shell |
|