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Yorodumi- PDB-4wia: Crystal structure of flagellar accessory protein FlaH from Methan... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4wia | ||||||
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Title | Crystal structure of flagellar accessory protein FlaH from Methanocaldococcus jannaschii | ||||||
Components | Putative flagella-related protein H | ||||||
Keywords | ATP-BINDING PROTEIN / archaea / flagella | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Methanocaldococcus jannaschii DSM 2661 (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.2 Å | ||||||
Authors | Meshcheryakov, V.A. / Wolf, M. | ||||||
Citation | Journal: Protein Sci. / Year: 2016 Title: Crystal structure of the flagellar accessory protein FlaH of Methanocaldococcus jannaschii suggests a regulatory role in archaeal flagellum assembly. Authors: Meshcheryakov, V.A. / Wolf, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4wia.cif.gz | 281.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4wia.ent.gz | 240.8 KB | Display | PDB format |
PDBx/mmJSON format | 4wia.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4wia_validation.pdf.gz | 472.6 KB | Display | wwPDB validaton report |
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Full document | 4wia_full_validation.pdf.gz | 485.4 KB | Display | |
Data in XML | 4wia_validation.xml.gz | 27.2 KB | Display | |
Data in CIF | 4wia_validation.cif.gz | 37 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wi/4wia ftp://data.pdbj.org/pub/pdb/validation_reports/wi/4wia | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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-Components
#1: Protein | Mass: 26368.836 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanocaldococcus jannaschii DSM 2661 (archaea) Gene: flaH, MJ0899 / Plasmid: pTXB1 / Production host: Escherichia coli (E. coli) / Strain (production host): B834 (DE3) / References: UniProt: Q58309 #2: Chemical | ChemComp-SO4 / #3: Chemical | ChemComp-GOL / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.88 % |
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Crystal grow | Temperature: 283 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 0.1 M HEPES, 0.65 M Rb2SO4 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL38B1 / Wavelength: 0.97886, 0.97944 | |||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 27, 2014 | |||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 2.2→50 Å / Num. obs: 45022 / % possible obs: 100 % / Redundancy: 10.6 % / Net I/σ(I): 11.9 |
-Processing
Software | Name: REFMAC / Version: 5.8.0073 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MAD / Resolution: 2.2→50 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.94 / SU B: 15.644 / SU ML: 0.185 / Cross valid method: THROUGHOUT / ESU R: 0.237 / ESU R Free: 0.187 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 68.069 Å2
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Refinement step | Cycle: 1 / Resolution: 2.2→50 Å
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Refine LS restraints |
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