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- PDB-4w5j: New structural conformations of adenylate kinase from Streptococc... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4w5j | ||||||
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Title | New structural conformations of adenylate kinase from Streptococcus pneumoniae D39 with Ap5A | ||||||
![]() | Adenylate kinase | ||||||
![]() | TRANSFERASE / adenylate kinase / NMP/LID domain / AMP/ATP binding / phosphotransferase | ||||||
Function / homology | ![]() CDP biosynthetic process / (d)CMP kinase activity / UMP kinase activity / adenylate kinase / adenylate kinase activity / AMP salvage / UDP biosynthetic process / nucleoside diphosphate kinase activity / ATP binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Thach, T.T. / Lee, S.H. | ||||||
![]() | ![]() Title: New crystal structures of adenylate kinase from Streptococcus pneumoniae D39 in two conformations. Authors: Thach, T.T. / Lee, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 497.5 KB | Display | ![]() |
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PDB format | ![]() | 417.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 45.3 KB | Display | |
Data in CIF | ![]() | 63.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4w5hC ![]() 4nu0S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24155.322 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: adk, SPD_0214 / Production host: ![]() ![]() #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-AP5 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.01 Å3/Da / Density % sol: 38.67 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 0.1 Sodium acetate, 0.1M Sodium acetate pH 4.6, 30%(v/v) PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RIGAKU JUPITER 210 / Detector: CCD / Date: Dec 13, 2011 |
Radiation | Monochromator: Fixed exit Si double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→46.99 Å / Num. obs: 85180 / % possible obs: 95.9 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.057 / Net I/σ(I): 39.02 |
Reflection shell | Resolution: 1.65→1.68 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.44 / Mean I/σ(I) obs: 4.43 / % possible all: 94.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4NU0 Resolution: 1.65→46.99 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / Phase error: 37.78
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Solvent computation | Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.26 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→46.99 Å
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Refine LS restraints |
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LS refinement shell |
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