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- PDB-4v84: Crystal structure of a complex containing domain 3 of CrPV IGR IR... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4v84 | |||||||||
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Title | Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. | |||||||||
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![]() | RIBOSOME / 70S / IGR / IRES / PSIV / CrPV | |||||||||
Function / homology | ![]() large ribosomal subunit / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit ...large ribosomal subunit / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Zhu, J. / Korostelev, A. / Costantino, D. / Noller, H.F. / Kieft, J.S. | |||||||||
![]() | ![]() Title: Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome. Authors: Zhu, J. / Korostelev, A. / Costantino, D.A. / Donohue, J.P. / Noller, H.F. / Kieft, J.S. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 7.1 MB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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Components
-RNA chain , 4 types, 8 molecules AACAAVCVBADABBDB
#1: RNA chain | Mass: 489042.562 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #22: RNA chain | Mass: 13788.186 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #23: RNA chain | Mass: 936302.125 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #24: RNA chain | Mass: 38553.000 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-30S ribosomal protein ... , 20 types, 40 molecules ABCBACCCADCDAECEAFCFAGCGAHCHAICIAJCJAKCKALCLAMCMANCNAOCOAPCP...
#2: Protein | Mass: 26987.271 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62662 #3: Protein | Mass: 22862.430 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62663 #4: Protein | Mass: 24242.254 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62664 #5: Protein | Mass: 16460.193 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62665 #6: Protein | Mass: 11988.753 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62666 #7: Protein | Mass: 17919.775 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62667 #8: Protein | Mass: 15868.570 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62668 #9: Protein | Mass: 14298.466 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62669 #10: Protein | Mass: 11299.176 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62653 #11: Protein | Mass: 12606.369 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62654 #12: Protein | Mass: 13804.311 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P61941 #13: Protein | Mass: 13237.430 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62655 #14: Protein | Mass: 7027.529 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62656 #15: Protein | Mass: 10447.213 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62657 #16: Protein | Mass: 9924.469 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62238 #17: Protein | Mass: 11721.919 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62658 #18: Protein | Mass: 8155.812 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62659 #19: Protein | Mass: 8949.435 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62660 #20: Protein | Mass: 10907.060 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62661 #21: Protein/peptide | Mass: 2960.475 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: HB27 / ATCC BAA-163 / DSM 7039 / References: UniProt: P62613 |
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+50S ribosomal protein ... , 29 types, 58 molecules BCDCBDDDBEDEBFDFBGDGBHDHBIDIBJDJBKDKBLDLBMDMBNDNBODOBPDPBQDQ...
-Non-polymers , 2 types, 1198 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/ZN.gif)
#54: Chemical | ChemComp-MG / #55: Chemical | ChemComp-ZN / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.44 Å3/Da / Density % sol: 64.2 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion / pH: 7 Details: 100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Mar 12, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 3.4→49.523 Å / Num. obs: 775795 |
-Phasing
Phasing | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 53.276 Å2 / ksol: 0.257 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 505.11 Å2 / Biso mean: 145.3711 Å2 / Biso min: 41.99 Å2
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Refinement step | Cycle: LAST / Resolution: 3.4→49.523 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30
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