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- PDB-4uzm: Shotgun proteolysis: A practical application -

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Basic information

Entry
Database: PDB / ID: 4uzm
TitleShotgun proteolysis: A practical application
ComponentsPUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG
KeywordsSTRUCTURAL PROTEIN
Function / homologyIntracellular growth attenuator IgaA / Intracellular growth attenuator protein IgaA / regulation of phosphorelay signal transduction system / phosphorelay response regulator activity / cellular response to cell envelope stress / plasma membrane / Putative membrane protein IgaA homolog
Function and homology information
Biological speciesESCHERICHIA COLI (E. coli)
MethodSOLUTION NMR / CNS
AuthorsAllen, M.D. / Bycroft, M. / Freund, S.M.V. / Christ, D.
CitationJournal: Protein Eng.Des.Sel. / Year: 2015
Title: Solution Structure of a Soluble Fragment Derived from a Membrane Protein by Shotgun Proteolysis.
Authors: Allen, M.D. / Christie, M. / Jones, P. / Porebski, B.T. / Roome, B. / Freund, S.M. / Buckle, A.M. / Bycroft, M. / Christ, D.
History
DepositionSep 5, 2014Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 17, 2014Provider: repository / Type: Initial release
Revision 1.1Sep 24, 2014Group: Atomic model
Revision 1.2Apr 29, 2015Group: Database references / Other
Revision 1.3Oct 21, 2015Group: Database references
Revision 1.4Apr 27, 2016Group: Atomic model / Other
Revision 1.5May 15, 2024Group: Data collection / Database references / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_cs / _pdbx_database_status.status_code_mr

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG


Theoretical massNumber of molelcules
Total (without water)13,4361
Polymers13,4361
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPQS
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 20NO VIOLATIONS
RepresentativeModel #1

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Components

#1: Protein PUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG / YRFF


Mass: 13435.933 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) ESCHERICHIA COLI (E. coli) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P45800

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: NONE

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Sample preparation

DetailsContents: 95% WATER/5% D2O
Sample conditionsIonic strength: 120 mM / pH: 6.5 / Temperature: 293.0 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker DRXBrukerDRX6001
Bruker DRXBrukerDRX8002

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Processing

NMR software
NameDeveloperClassification
CNSBRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,READ, RICE,SIMONSON,WARRENrefinement
ANSIGstructure solution
CNSstructure solution
RefinementMethod: CNS / Software ordinal: 1
NMR ensembleConformer selection criteria: NO VIOLATIONS / Conformers calculated total number: 20 / Conformers submitted total number: 20

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