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Open data
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Basic information
Entry | Database: PDB / ID: 4uuj | ||||||
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Title | POTASSIUM CHANNEL KCSA-FAB WITH TETRAHEXYLAMMONIUM | ||||||
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![]() | IMMUNE SYSTEM/METAL TRANSPORT / IMMUNE SYSTEM-METAL TRANSPORT COMPLEX / QUATERNARY AMMONIUM / PROTEIN-ANTIBODY FAB COMPLEX / IONIC CHANNEL / ION TRANSPORT / POTASSIUM CHANNEL | ||||||
Function / homology | ![]() delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lenaeus, M.J. / Burdette, D. / Wagner, T. / Focia, P.J. / Gross, A. | ||||||
![]() | ![]() Title: Structures of Kcsa in Complex with Symmetrical Quaternary Ammonium Compounds Reveal a Hydrophobic Binding Site. Authors: Lenaeus, M.J. / Burdette, D. / Wagner, T. / Focia, P.J. / Gross, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 128.6 KB | Display | ![]() |
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PDB format | ![]() | 97.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.4 MB | Display | ![]() |
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Full document | ![]() | 2.4 MB | Display | |
Data in XML | ![]() | 25.6 KB | Display | |
Data in CIF | ![]() | 34.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2jk5C ![]() 2w0fC ![]() 1k4cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 11687.627 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Non-polymers , 8 types, 101 molecules ![](data/chem/img/F09.gif)
![](data/chem/img/IND.gif)
![](data/chem/img/K.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/CO.gif)
![](data/chem/img/XA7.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/IND.gif)
![](data/chem/img/K.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/CO.gif)
![](data/chem/img/XA7.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-F09 / #5: Chemical | ChemComp-IND / | #6: Chemical | ChemComp-K / #7: Chemical | ChemComp-DGA / | #8: Chemical | ChemComp-CO / | #9: Chemical | #10: Chemical | ChemComp-PO4 / | #11: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.71 Å3/Da / Density % sol: 66.8 % / Description: NONE |
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Crystal grow | pH: 5.4 Details: CRYSTALLIZATION CONDITIONS: 18-25% PEG400, 50 MM SODIUM REMARK 280 ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Nov 19, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 32854 / % possible obs: 98.1 % / Observed criterion σ(I): -3 / Redundancy: 3.31 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 17.1 |
Reflection shell | Resolution: 2.4→2.54 Å / Redundancy: 3.31 % / Rmerge(I) obs: 0.58 / Mean I/σ(I) obs: 2.1 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1K4C Resolution: 2.4→28.64 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.938 / SU B: 10.451 / SU ML: 0.223 / Cross valid method: THROUGHOUT / ESU R: 0.252 / ESU R Free: 0.223 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 71.704 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→28.64 Å
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Refine LS restraints |
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