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- PDB-4ucm: CRYSTAL STRUCTURE OF LEISHMANIA MAJOR N-MYRISTOYLTRANSFERASE (NMT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ucm | ||||||
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Title | CRYSTAL STRUCTURE OF LEISHMANIA MAJOR N-MYRISTOYLTRANSFERASE (NMT) WITH BOUND MYRISTOYL-COA AND A FRAGMENT | ||||||
![]() | GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE | ||||||
![]() | TRANSFERASE / NMT / MYRISTOYLTRANSFERASE / ACYLTRANSFERASE / DRUG DISCOVERY / FRAGMENT | ||||||
Function / homology | ![]() glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Robinson, D.A. / Wyatt, P.G. | ||||||
![]() | ![]() Title: Identification and Structure Solution of Fragment Hits Against Kinetoplastid N-Myristoyltransferase Authors: Robinson, D.A. / Wyatt, P.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 183.7 KB | Display | ![]() |
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PDB format | ![]() | 144 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 676.7 KB | Display | ![]() |
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Full document | ![]() | 677.5 KB | Display | |
Data in XML | ![]() | 17.4 KB | Display | |
Data in CIF | ![]() | 24.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ucnC ![]() 4ucpC ![]() 3h5zS C: citing same article ( S: Starting model for refinement |
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Similar structure data | |
Experimental dataset #1 | Data reference: ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50513.254 Da / Num. of mol.: 1 / Fragment: RESIDUES 5-421 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q4Q5S8, glycylpeptide N-tetradecanoyltransferase |
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#2: Chemical | ChemComp-MYA / |
#3: Chemical | ChemComp-X6W / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 0.46 % / Description: NONE |
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Crystal grow | pH: 5.5 / Details: 25.9%PEG1500,0.2M NACL, 0.1M NACACODYLATE, PH 5.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Dec 6, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97887 Å / Relative weight: 1 |
Reflection | Resolution: 2.32→44 Å / Num. obs: 17931 / % possible obs: 97.4 % / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Rmerge(I) obs: 0.03 / Net I/σ(I): 24 |
Reflection shell | Resolution: 2.32→2.4 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.08 / Mean I/σ(I) obs: 10 / % possible all: 87.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3H5Z Resolution: 2.32→49.51 Å / Cor.coef. Fo:Fc: 0.935 / Cor.coef. Fo:Fc free: 0.893 / SU B: 16.809 / SU ML: 0.197 / Cross valid method: THROUGHOUT / ESU R: 0.575 / ESU R Free: 0.293 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 41.021 Å2
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Refinement step | Cycle: LAST / Resolution: 2.32→49.51 Å
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Refine LS restraints |
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