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Yorodumi- PDB-4u1p: Human Fyn-SH2 domain in complex with a synthetic high-affinity ph... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4u1p | ||||||
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Title | Human Fyn-SH2 domain in complex with a synthetic high-affinity phospho-peptide | ||||||
Components |
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Keywords | TRANSFERASE / SH2 domain / protein peptide interactions / allostery / tyrosine phosphorylation / Fyn / kinase | ||||||
Function / homology | Function and homology information response to singlet oxygen / Reelin signalling pathway / negative regulation of hydrogen peroxide biosynthetic process / perinuclear endoplasmic reticulum / NTRK2 activates RAC1 / growth factor receptor binding / Activated NTRK2 signals through FYN / heart process / cellular response to L-glutamate / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion ...response to singlet oxygen / Reelin signalling pathway / negative regulation of hydrogen peroxide biosynthetic process / perinuclear endoplasmic reticulum / NTRK2 activates RAC1 / growth factor receptor binding / Activated NTRK2 signals through FYN / heart process / cellular response to L-glutamate / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / regulation of glutamate receptor signaling pathway / regulation of calcium ion import across plasma membrane / Platelet Adhesion to exposed collagen / CD28 co-stimulation / positive regulation of protein localization to membrane / activated T cell proliferation / CRMPs in Sema3A signaling / positive regulation of cysteine-type endopeptidase activity / FLT3 signaling through SRC family kinases / Nef and signal transduction / feeding behavior / negative regulation of dendritic spine maintenance / type 5 metabotropic glutamate receptor binding / DCC mediated attractive signaling / Nephrin family interactions / dendrite morphogenesis / EPH-Ephrin signaling / CD28 dependent Vav1 pathway / Ephrin signaling / dendritic spine maintenance / Regulation of KIT signaling / CTLA4 inhibitory signaling / tau-protein kinase activity / phospholipase activator activity / leukocyte migration / EPHA-mediated growth cone collapse / Fc-gamma receptor signaling pathway involved in phagocytosis / cellular response to platelet-derived growth factor stimulus / cellular response to glycine / Dectin-2 family / glial cell projection / stimulatory C-type lectin receptor signaling pathway / phospholipase binding / PECAM1 interactions / CD28 dependent PI3K/Akt signaling / response to amyloid-beta / alpha-tubulin binding / Sema3A PAK dependent Axon repulsion / positive regulation of protein targeting to membrane / FCGR activation / EPH-ephrin mediated repulsion of cells / host cell membrane / ephrin receptor signaling pathway / Role of LAT2/NTAL/LAB on calcium mobilization / vascular endothelial growth factor receptor signaling pathway / detection of mechanical stimulus involved in sensory perception of pain / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / forebrain development / regulation of peptidyl-tyrosine phosphorylation / positive regulation of tyrosine phosphorylation of STAT protein / negative regulation of protein ubiquitination / negative regulation of inflammatory response to antigenic stimulus / Signaling by ERBB2 / GPVI-mediated activation cascade / cellular response to transforming growth factor beta stimulus / EPHB-mediated forward signaling / T cell costimulation / extrinsic component of cytoplasmic side of plasma membrane / NCAM signaling for neurite out-growth / CD209 (DC-SIGN) signaling / ephrin receptor binding / FCGR3A-mediated IL10 synthesis / Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / learning / actin filament / axon guidance / Regulation of signaling by CBL / Cell surface interactions at the vascular wall / FCGR3A-mediated phagocytosis / neuron migration / protein catabolic process / non-specific protein-tyrosine kinase / tau protein binding / modulation of chemical synaptic transmission / Schaffer collateral - CA1 synapse / non-membrane spanning protein tyrosine kinase activity / negative regulation of protein catabolic process / Signaling by SCF-KIT / positive regulation of neuron projection development / cellular response to hydrogen peroxide / VEGFA-VEGFR2 Pathway / positive regulation of protein localization to nucleus / peptidyl-tyrosine phosphorylation / cellular response to amyloid-beta / cell surface receptor protein tyrosine kinase signaling pathway / Constitutive Signaling by Aberrant PI3K in Cancer / calcium ion transport / Signaling by CSF1 (M-CSF) in myeloid cells / disordered domain specific binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Hamster polyomavirus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.4 Å | ||||||
Authors | Garcia-Pino, A. / Huculeci, R. / Lenaerts, T. / van Nuland, N.A.J. | ||||||
Citation | Journal: To Be Published Title: Human Fyn-SH2 domain in complex with a synthetic high-affinity phospho-peptide Authors: Garcia-Pino, A. / Huculeci, R. / Lenaerts, T. / van Nuland, N.A.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4u1p.cif.gz | 78.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4u1p.ent.gz | 53.8 KB | Display | PDB format |
PDBx/mmJSON format | 4u1p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u1/4u1p ftp://data.pdbj.org/pub/pdb/validation_reports/u1/4u1p | HTTPS FTP |
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-Related structure data
Related structure data | 1g83S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14122.016 Da / Num. of mol.: 1 / Fragment: Human Fyn-SH2 domain, RESIDUES 148-248 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FYN / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: P06241, non-specific protein-tyrosine kinase |
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#2: Protein/peptide | Mass: 1473.515 Da / Num. of mol.: 1 / Fragment: RESIDUES 320-330 / Source method: obtained synthetically / Source: (synth.) Hamster polyomavirus / References: UniProt: K0F5T5, UniProt: P03079*PLUS |
#3: Chemical | ChemComp-15P / |
#4: Chemical | ChemComp-NA / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.79 Å3/Da / Density % sol: 31.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.1 M MOPS/Na HEPES pH 7.5, 0.12 M monosaccharide mixture, PEG 1000, 12.5%(w/v) PEG 3350 and 12.5%(v/v) MPD PH range: 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.9334 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 30, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9334 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→23.34 Å / Num. obs: 23359 / % possible obs: 99.93 % / Redundancy: 14.7 % / Net I/σ(I): 24.43 |
Reflection shell | Resolution: 1.4→1.45 Å / Redundancy: 13.3 % / Rmerge(I) obs: 0.361 / Mean I/σ(I) obs: 7.73 / % possible all: 99.96 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1G83 Resolution: 1.4→23 Å / SU ML: 0.12 / Cross valid method: THROUGHOUT / Phase error: 16.75 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→23 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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