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- PDB-4ttx: N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p variant ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ttx | ||||||
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Title | N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p variant G94C K146C (NN19) | ||||||
![]() | Centriole protein | ||||||
![]() | STRUCTURAL PROTEIN / homodimer / stabilization / mutation / centriole SAS-6 / cartwheel / beta-sandwich / alpha-beta protein / centriolar | ||||||
Function / homology | Spindle assembly abnormal protein 6, N-terminal / SAS-6, N-terminal domain superfamily / Centriolar protein SAS N-terminal domain / centriole / cell cycle / identical protein binding / cytoplasm / Centriole protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hilbert, M. / Kraatz, S.H.W. | ||||||
Funding support | ![]()
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![]() | ![]() Title: SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Authors: Hilbert, M. / Noga, A. / Frey, D. / Hamel, V. / Guichard, P. / Kraatz, S.H. / Pfreundschuh, M. / Hosner, S. / Fluckiger, I. / Jaussi, R. / Wieser, M.M. / Thieltges, K.M. / Deupi, X. / ...Authors: Hilbert, M. / Noga, A. / Frey, D. / Hamel, V. / Guichard, P. / Kraatz, S.H. / Pfreundschuh, M. / Hosner, S. / Fluckiger, I. / Jaussi, R. / Wieser, M.M. / Thieltges, K.M. / Deupi, X. / Muller, D.J. / Kammerer, R.A. / Gonczy, P. / Hirono, M. / Steinmetz, M.O. #1: ![]() Title: Structural basis of the 9-fold symmetry of centrioles. Authors: Kitagawa, D. / Vakonakis, I. / Olieric, N. / Hilbert, M. / Keller, D. / Olieric, V. / Bortfeld, M. / Erat, M.C. / Flueckiger, I. / Goenczy, P. / Steinmetz, M.O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 202 KB | Display | ![]() |
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PDB format | ![]() | 161.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 449.1 KB | Display | ![]() |
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Full document | ![]() | 453 KB | Display | |
Data in XML | ![]() | 39.6 KB | Display | |
Data in CIF | ![]() | 57.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4to7C ![]() 4tpzC ![]() 4tq7C ![]() 4ttwC ![]() 4ttyC ![]() 4ttzC ![]() 4u2iC ![]() 4u2jC ![]() 3q0yS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18123.674 Da / Num. of mol.: 6 / Fragment: N-terminal domain, Residues 1-159 / Mutation: G94C K146C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.25 Å3/Da / Density % sol: 62.13 % |
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Crystal grow | Temperature: 293 K / Method: evaporation / Details: PEG6000, MES-NaOH |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 9, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→63.26 Å / Num. obs: 48246 / % possible obs: 99.6 % / Redundancy: 3.8 % / Net I/σ(I): 6.78 |
Reflection shell | Resolution: 2.5→2.56 Å / Redundancy: 3.7 % / Rmerge(I) obs: 2.017 / Mean I/σ(I) obs: 0.72 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3Q0Y Resolution: 2.498→63.257 Å / SU ML: 0.39 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.79 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.498→63.257 Å
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Refine LS restraints |
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LS refinement shell |
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