+Open data
-Basic information
Entry | Database: PDB / ID: 4rz2 | ||||||
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Title | Crystal structure of the MinD-like ATPase FlhG | ||||||
Components | Site-determining protein | ||||||
Keywords | STRUCTURAL PROTEIN / P-loop / Walker A / SIMIBI / flagellum / cell division | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Geobacillus thermodenitrificans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Schuhmacher, J.S. / Bange, G. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2015 Title: MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly. Authors: Schuhmacher, J.S. / Rossmann, F. / Dempwolff, F. / Knauer, C. / Altegoer, F. / Steinchen, W. / Dorrich, A.K. / Klingl, A. / Stephan, M. / Linne, U. / Thormann, K.M. / Bange, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4rz2.cif.gz | 110.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4rz2.ent.gz | 84.7 KB | Display | PDB format |
PDBx/mmJSON format | 4rz2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4rz2_validation.pdf.gz | 435.6 KB | Display | wwPDB validaton report |
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Full document | 4rz2_full_validation.pdf.gz | 441.6 KB | Display | |
Data in XML | 4rz2_validation.xml.gz | 20.2 KB | Display | |
Data in CIF | 4rz2_validation.cif.gz | 27.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rz/4rz2 ftp://data.pdbj.org/pub/pdb/validation_reports/rz/4rz2 | HTTPS FTP |
-Related structure data
Related structure data | 4rz3C 3ql9S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 32660.453 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Geobacillus thermodenitrificans (bacteria) Strain: NG80, NG80-2 / Gene: GTNG_1094 / Plasmid: pET24d / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) / References: UniProt: A4IMB4 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.67 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.1 M HEPES, pH 7.5, 10 % (w/v) PEG 8000, 0.1 M Urea, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.873 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 23, 2011 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.873 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→51.96 Å / Num. obs: 16375 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.1 % / Rmerge(I) obs: 0.136 / Net I/σ(I): 11.7 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 5.2 % / Rmerge(I) obs: 0.47 / Mean I/σ(I) obs: 5.2 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3QL9 Resolution: 2.8→47.416 Å / SU ML: 0.31 / σ(F): 0.03 / Phase error: 21.27 / Stereochemistry target values: MLHL
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→47.416 Å
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Refine LS restraints |
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LS refinement shell |
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