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Open data
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Basic information
Entry | Database: PDB / ID: 4rxf | ||||||
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Title | Fructose-6-phosphate aldolase Y131F from E.coli | ||||||
![]() | Fructose-6-phosphate aldolase 1 | ||||||
![]() | LYASE / Tim barrel / homodecamer / FSAA TalB | ||||||
Function / homology | ![]() ketone catabolic process / fructose 6-phosphate aldolase activity / Lyases; Carbon-carbon lyases; Aldehyde-lyases / fructose metabolic process / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Stellmacher, L. / Sandalova, T. / Leptihn, S. / Schneider, G. / Sprenger, G.A. / Samland, A.K. | ||||||
![]() | ![]() Title: Acid Base Catalyst Discriminates between a Fructose 6-Phosphate Aldolase and a Transaldolase Authors: Stellmacher, L. / Sandalova, T. / Leptihn, S. / Schneider, G. / Sprenger, G.A. / Samland, A.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 395.3 KB | Display | ![]() |
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PDB format | ![]() | 325.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 488.2 KB | Display | ![]() |
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Full document | ![]() | 495.8 KB | Display | |
Data in XML | ![]() | 69.7 KB | Display | |
Data in CIF | ![]() | 95.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4rxgC ![]() 4rz4C ![]() 4rz5C ![]() 4rz6C ![]() 4s1fC ![]() 1l6wS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 23829.637 Da / Num. of mol.: 10 / Mutation: Y131F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P78055, Lyases; Carbon-carbon lyases; Aldehyde-lyases #2: Chemical | ChemComp-PO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.1 % |
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Crystal grow | Temperature: 293.2 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 19% PEG3350, 0.1M HEPES pH 8, 0.2M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 14, 2014 |
Radiation | Monochromator: Si111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91844 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→101.49 Å / Num. all: 91562 / Num. obs: 91562 / % possible obs: 99.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.8 % / Rmerge(I) obs: 0.105 / Net I/σ(I): 12.7 |
Reflection shell | Resolution: 2.4→2.44 Å / Redundancy: 6 % / Rmerge(I) obs: 0.465 / Mean I/σ(I) obs: 3.7 / % possible all: 98.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1L6W Resolution: 2.4→101.49 Å / Cor.coef. Fo:Fc: 0.936 / Cor.coef. Fo:Fc free: 0.905 / SU B: 8.099 / SU ML: 0.185 / Cross valid method: THROUGHOUT / ESU R: 0.416 / ESU R Free: 0.249 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.08 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→101.49 Å
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Refine LS restraints |
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