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- PDB-4rsj: Pyrococcus furiosus Smc hinge domain with an extended coiled coil -
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Open data
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Basic information
Entry | Database: PDB / ID: 4rsj | ||||||
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Title | Pyrococcus furiosus Smc hinge domain with an extended coiled coil | ||||||
![]() | Chromosome partition protein Smc | ||||||
![]() | CELL CYCLE / Smc hinge domain with coiled coils | ||||||
Function / homology | ![]() cohesin loader activity / chromosome condensation / mitotic sister chromatid cohesion / chromosome segregation / chromosome / double-stranded DNA binding / DNA replication / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Soh, Y.M. / Shin, H.C. / Oh, B.H. | ||||||
![]() | ![]() Title: Molecular Basis for SMC Rod Formation and Its Dissolution upon DNA Binding. Authors: Soh, Y.M. / Burmann, F. / Shin, H.C. / Oda, T. / Jin, K.S. / Toseland, C.P. / Kim, C. / Lee, H. / Kim, S.J. / Kong, M.S. / Durand-Diebold, M.L. / Kim, Y.G. / Kim, H.M. / Lee, N.K. / Sato, M. ...Authors: Soh, Y.M. / Burmann, F. / Shin, H.C. / Oda, T. / Jin, K.S. / Toseland, C.P. / Kim, C. / Lee, H. / Kim, S.J. / Kong, M.S. / Durand-Diebold, M.L. / Kim, Y.G. / Kim, H.M. / Lee, N.K. / Sato, M. / Oh, B.H. / Gruber, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 205.5 KB | Display | ![]() |
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PDB format | ![]() | 162.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 432.9 KB | Display | ![]() |
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Full document | ![]() | 463.8 KB | Display | |
Data in XML | ![]() | 25.7 KB | Display | |
Data in CIF | ![]() | 37.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4rsiC ![]() 3nwcS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30860.355 Da / Num. of mol.: 4 / Fragment: Smc hinge Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.51 Å3/Da / Density % sol: 64.96 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 9.5 Details: 1 M Sodium Citrate, 0.1 M CHES pH 9.5, 8% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 30, 2013 |
Radiation | Monochromator: Double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→50 Å / Num. all: 20012 / Num. obs: 18608 / % possible obs: 89.5 % / Rsym value: 0.093 / Net I/σ(I): 26.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3NWC Resolution: 3.5→50 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 3.5→50 Å
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Refine LS restraints |
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