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- PDB-4rsh: Structure of a putative lipolytic protein of G-D-S-L family from ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4rsh | ||||||
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Title | Structure of a putative lipolytic protein of G-D-S-L family from Desulfitobacterium hafniense DCB-2 | ||||||
![]() | Lipolytic protein G-D-S-L family | ||||||
![]() | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PSI-Biology / Midwest Center for Structural Genomics / MCSG | ||||||
Function / homology | SGNH hydrolase / SGNH hydrolase-type esterase domain / GDSL-like Lipase/Acylhydrolase family / SGNH hydrolase superfamily / membrane => GO:0016020 / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / Lipolytic protein G-D-S-L family![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Filippova, E.V. / Wawrzak, Z. / Minasov, G. / Kiryukhina, O. / Endres, M. / Joachimiak, A. / Anderson, W.F. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: Structure of a putative lipolytic protein of G-D-S-L family from Desulfitobacterium hafniense DCB-2 Authors: Filippova, E.V. / Wawrzak, Z. / Minasov, G. / Kiryukhina, O. / Endres, M. / Joachimiak, A. / Anderson, W.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 216.6 KB | Display | ![]() |
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PDB format | ![]() | 184.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.9 KB | Display | ![]() |
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Full document | ![]() | 438.9 KB | Display | |
Data in XML | ![]() | 23.4 KB | Display | |
Data in CIF | ![]() | 34 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 19994.062 Da / Num. of mol.: 3 / Fragment: residues 53-228 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DCB-2 / Gene: ACL22416, Dhaf_4411 / Plasmid: pMCSG68 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.44 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.1 M Potassium Thiocyanate, 30% PEG MME 2000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jul 21, 2014 / Details: beryllium lenses |
Radiation | Monochromator: C(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97872 Å / Relative weight: 1 |
Reflection | Resolution: 2.19→85.65 Å / Num. all: 29332 / Num. obs: 29332 / % possible obs: 98.9 % / Observed criterion σ(I): -3 / Redundancy: 13.6 % / Rmerge(I) obs: 0.199 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 2.19→2.4 Å / Redundancy: 11.1 % / Rmerge(I) obs: 0.84 / Mean I/σ(I) obs: 3 / % possible all: 95.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.771 Å2
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Refinement step | Cycle: LAST / Resolution: 2.19→30 Å
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Refine LS restraints |
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