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- PDB-4rmb: Crystal structure of keratin 4 binding domain of surface adhesin ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4rmb | ||||||
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Title | Crystal structure of keratin 4 binding domain of surface adhesin Srr-1 of S.agalactiae | ||||||
![]() | Serine rich repeat protein-1 (Srr-1) | ||||||
![]() | CELL ADHESION / Variant of DEv-IgG fold / adhesin / keratin 4 / bacterial cell surface / beta sheet complementation | ||||||
Function / homology | ![]() keratin filament binding / symbiont entry into host / cell adhesion / cell surface / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ponnuraj, K. / Sundaresan, R. | ||||||
![]() | ![]() Title: Structure of KRT4 binding domain of Srr-1 from Streptococcus agalactiae reveals a novel beta-sheet complementation. Authors: Sundaresan, R. / Samen, U. / Ponnuraj, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70 KB | Display | ![]() |
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PDB format | ![]() | 52.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.6 KB | Display | ![]() |
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Full document | ![]() | 442.7 KB | Display | |
Data in XML | ![]() | 13.9 KB | Display | |
Data in CIF | ![]() | 18.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4mboS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18526.262 Da / Num. of mol.: 2 / Fragment: Keratin 4 binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: NEM316 / Gene: gbs1529 / Plasmid: pET28a / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.78 Å3/Da / Density % sol: 31 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.7 Details: 25% (w/v) PEG 3350 (Sigma), 100 mM Lithium chloride and 100 mM Imidazole, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Oct 27, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97927 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→35.95 Å / Num. all: 27350 / Num. obs: 27350 / % possible obs: 95.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.8 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 14.1 |
Reflection shell | Resolution: 1.7→1.79 Å / Redundancy: 5.8 % / Rmerge(I) obs: 0.66 / Num. unique all: 27350 / % possible all: 80.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4MBO Resolution: 1.7→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 24.22 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→20 Å
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