+Open data
-Basic information
Entry | Database: PDB / ID: 4rcb | ||||||
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Title | Crystal structure of E Coli Hfq | ||||||
Components | RNA-binding protein Hfq | ||||||
Keywords | RNA BINDING PROTEIN | ||||||
Function / homology | Function and homology information sRNA-mediated post-transcriptional gene silencing / positive regulation of translation, ncRNA-mediated / regulation of translation, ncRNA-mediated / RNA folding chaperone / bent DNA binding / regulation of RNA stability / regulation of DNA-templated transcription / DNA binding / RNA binding / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli K-12 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.63 Å | ||||||
Authors | Su, J.Y. | ||||||
Citation | Journal: Chin J Biochem Mol Biol / Year: 2015 Title: Limited proteolysis improves E.coli Hfq crystal structure resolution Authors: Feng, S.Q. / Si, Y.L. / Song, C.Y. / Wang, P.Q. / Su, J.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4rcb.cif.gz | 25 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4rcb.ent.gz | 15.9 KB | Display | PDB format |
PDBx/mmJSON format | 4rcb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rc/4rcb ftp://data.pdbj.org/pub/pdb/validation_reports/rc/4rcb | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 7653.859 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 5-71 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A6X3 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.3 % |
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Crystal grow | Method: vapor diffusion, sitting drop Details: 0.5M Lithium chloride, 1.6M Ammonium sulfate , VAPOR DIFFUSION, SITTING DROP |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.97159 Å |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97159 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.63 Å / Num. obs: 7684 |
-Processing
Software | Name: PHENIX / Version: (phenix.refine: 1.9_1692) / Classification: refinement | ||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.63→26.531 Å / SU ML: 0.19 / σ(F): 1.36 / Phase error: 28.24 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.63→26.531 Å
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Refine LS restraints |
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LS refinement shell |
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