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- PDB-4q9n: Crystal structure of Chlamydia trachomatis enoyl-ACP reductase (F... -

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Basic information

Entry
Database: PDB / ID: 4q9n
TitleCrystal structure of Chlamydia trachomatis enoyl-ACP reductase (FabI) in complex with NADH and AFN-1252
ComponentsEnoyl-[acyl-carrier-protein] reductase [NADH]
Keywordsoxidoreductase/oxidoreductase inhibitor / Enoyl-ACP reductase / NADH / AFN-1252 / fatty acid synthesis / Chlamydia / oxidoreductase-oxidoreductase inhibitor complex
Function / homologyNAD(P)-binding Rossmann-like Domain / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / Chem-0WE / 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / :
Function and homology information
Biological speciesChlamydia trachomatis F/11-96 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.795 Å
AuthorsYao, J. / Abdelrahman, Y. / Robertson, R.M. / Cox, J.V. / Belland, R.J. / White, S.W. / Rock, C.O.
CitationJournal: J.Biol.Chem. / Year: 2014
Title: Type II Fatty Acid Synthesis Is Essential for the Replication of Chlamydia trachomatis.
Authors: Yao, J. / Abdelrahman, Y.M. / Robertson, R.M. / Cox, J.V. / Belland, R.J. / White, S.W. / Rock, C.O.
History
DepositionMay 1, 2014Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 25, 2014Provider: repository / Type: Initial release
Revision 1.1Aug 27, 2014Group: Database references
Revision 1.2Nov 22, 2017Group: Refinement description / Category: software
Revision 1.3Sep 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Enoyl-[acyl-carrier-protein] reductase [NADH]
B: Enoyl-[acyl-carrier-protein] reductase [NADH]
C: Enoyl-[acyl-carrier-protein] reductase [NADH]
D: Enoyl-[acyl-carrier-protein] reductase [NADH]
E: Enoyl-[acyl-carrier-protein] reductase [NADH]
F: Enoyl-[acyl-carrier-protein] reductase [NADH]
G: Enoyl-[acyl-carrier-protein] reductase [NADH]
H: Enoyl-[acyl-carrier-protein] reductase [NADH]
hetero molecules


Theoretical massNumber of molelcules
Total (without water)264,26724
Polymers255,9248
Non-polymers8,34316
Water17,222956
1
A: Enoyl-[acyl-carrier-protein] reductase [NADH]
B: Enoyl-[acyl-carrier-protein] reductase [NADH]
C: Enoyl-[acyl-carrier-protein] reductase [NADH]
D: Enoyl-[acyl-carrier-protein] reductase [NADH]
hetero molecules


Theoretical massNumber of molelcules
Total (without water)132,13412
Polymers127,9624
Non-polymers4,1728
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area22080 Å2
ΔGint-155 kcal/mol
Surface area37220 Å2
MethodPISA
2
E: Enoyl-[acyl-carrier-protein] reductase [NADH]
F: Enoyl-[acyl-carrier-protein] reductase [NADH]
G: Enoyl-[acyl-carrier-protein] reductase [NADH]
H: Enoyl-[acyl-carrier-protein] reductase [NADH]
hetero molecules


Theoretical massNumber of molelcules
Total (without water)132,13412
Polymers127,9624
Non-polymers4,1728
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area22150 Å2
ΔGint-152 kcal/mol
Surface area37070 Å2
MethodPISA
Unit cell
Length a, b, c (Å)96.146, 96.146, 263.082
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number78
Space group name H-MP43

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Components

#1: Protein
Enoyl-[acyl-carrier-protein] reductase [NADH]


Mass: 31990.541 Da / Num. of mol.: 8 / Fragment: FabI
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chlamydia trachomatis F/11-96 (bacteria)
Strain: D/UW-3/CX / Gene: fabI(CT104), O173_00565 / Plasmid: pET21 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Tuner
References: UniProt: T1TQB5, enoyl-[acyl-carrier-protein] reductase (NADH)
#2: Chemical
ChemComp-NAI / 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / NADH


Mass: 665.441 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C21H29N7O14P2
#3: Chemical
ChemComp-0WE / N-methyl-N-[(3-methyl-1-benzofuran-2-yl)methyl]-3-(7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-3-yl)propanamide


Mass: 377.436 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C22H23N3O3
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 956 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.38 Å3/Da / Density % sol: 48.22 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 0.1 M Hepes, 28% PEG300, 0.2M potassium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 6, 2014 / Details: mirrors
RadiationMonochromator: double crystal / Protocol: SINGLE WAVELENGTH / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.8→50 Å / Num. all: 218541 / Num. obs: 218541 / % possible obs: 98.7 % / Observed criterion σ(I): -3 / Redundancy: 6.3 % / Biso Wilson estimate: 20.45 Å2 / Rmerge(I) obs: 0.122 / Χ2: 1.232 / Net I/σ(I): 6.4
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2Diffraction-ID% possible all
1.8-1.853.40.616175000.696195.1
1.85-1.914.70.588182420.777199.1
1.91-1.9860.529184010.809199.9
1.98-2.066.90.439184240.8651100
2.06-2.157.20.362184120.919199.9
2.15-2.277.10.282183761.041199.7
2.27-2.4170.224183491.188199.6
2.41-2.66.90.181182541.093199.1
2.6-2.866.70.134181431.578198.4
2.86-3.276.30.103180501.864197.8
3.27-4.125.70.075179831.966197.3
4.12-5070.062184071.687199

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Phasing

PhasingMethod: molecular replacement
Phasing MRModel details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation2.7 Å48.07 Å
Translation2.7 Å48.07 Å

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
PHASER2.5.1phasing
PHENIX1.7.3_928refinement
PDB_EXTRACT3.14data extraction
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1EN0

1en0
PDB Unreleased entry


Resolution: 1.795→38.607 Å / FOM work R set: 0.8329 / SU ML: 0.26 / σ(F): 1.36 / Phase error: 24.36 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2292 10931 5.01 %Random
Rwork0.1863 ---
obs0.1884 218306 98.64 %-
Solvent computationShrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 30.455 Å2 / ksol: 0.328 e/Å3
Displacement parametersBiso max: 83.81 Å2 / Biso mean: 25.43 Å2 / Biso min: 8.14 Å2
Baniso -1Baniso -2Baniso -3
1--5.2808 Å2-0 Å20 Å2
2---5.2808 Å2-0 Å2
3---10.5616 Å2
Refinement stepCycle: LAST / Resolution: 1.795→38.607 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17700 0 576 956 19232
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00618740
X-RAY DIFFRACTIONf_angle_d1.05725529
X-RAY DIFFRACTIONf_chiral_restr0.0692832
X-RAY DIFFRACTIONf_plane_restr0.0053181
X-RAY DIFFRACTIONf_dihedral_angle_d11.9036631
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.7949-1.81530.34183330.32616448678191
1.8153-1.83670.32933480.30876732708096
1.8367-1.8590.33343740.28646813718798
1.859-1.88260.31883600.26576932729299
1.8826-1.90740.32953700.251769287298100
1.9074-1.93350.29593590.226970097368100
1.9335-1.96110.26913710.213570417412100
1.9611-1.99040.27323490.214769487297100
1.9904-2.02150.23923820.193469467328100
2.0215-2.05460.27073740.19870337407100
2.0546-2.090.26253650.198370547419100
2.09-2.1280.24833970.193569127309100
2.128-2.1690.26683660.19869417307100
2.169-2.21320.23893440.183969937337100
2.2132-2.26140.24123790.179870127391100
2.2614-2.3140.24223500.174270027352100
2.314-2.37180.25343860.18546903728999
2.3718-2.43590.24213460.18476985733199
2.4359-2.50760.24853550.196971732699
2.5076-2.58850.25893800.19456936731699
2.5885-2.6810.23993580.19066902726099
2.681-2.78830.23163460.186922726898
2.7883-2.91520.2253730.19296875724898
2.9152-3.06880.22994000.19486787718798
3.0688-3.2610.22433600.18946858721898
3.261-3.51260.23243690.19266835720498
3.5126-3.86580.21443370.17566864720197
3.8658-4.42450.17683680.14696777714597
4.4245-5.57180.16113510.14127011736299
5.5718-38.61570.173810.16467005738699
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.10940.2892-0.52011.0801-0.18370.9362-0.1599-0.0857-0.47630.02760.04490.06910.18570.00340.04360.1465-0.02150.03510.14740.07930.2223102.7552-16.538710.2064
21.9167-0.73870.26121.8499-0.09532.63750.0798-0.1733-0.3996-0.0195-0.07230.44930.1696-0.4897-0.17010.0889-0.03470.04580.15760.080.135589.4946-7.064211.8602
31.36890.19370.65360.7450.22933.4235-0.019-0.001-0.05910.00440.0310.10180.0046-0.0654-0.02010.10.00080.01160.08290.02380.0873102.12862.1820.7445
41.4258-0.3188-0.30241.4850.44581.86940.04390.1611-0.1915-0.1804-0.04650.08840.1293-0.1636-0.02910.0978-0.012-0.01490.09010.01090.1169115.3336-7.5352-3.7685
50.5813-0.01650.70790.8195-0.29841.31750.02310.20650.2365-0.10580.14560.1533-0.19040.0254-0.08570.18530.02740.02040.27220.13920.1812100.122628.8796-21.2829
61.0499-0.5161-0.01860.37050.0310.83680.13210.17650.4221-0.03240.07980.2631-0.3776-0.1175-0.08280.11020.14580.0630.16450.16330.426792.330131.9243-16.641
71.2426-0.6028-0.69141.14021.21731.70130.01380.0558-0.12790.086-0.15090.6076-0.111-0.46460.02940.14220.09410.09340.31980.10290.684877.056118.458-9.1432
81.5582-0.0702-0.47680.69350.21182.5520.02230.4546-0.0797-0.21730.0720.6138-0.0174-0.3245-0.05910.11630.0052-0.06350.32070.11570.344989.710719.4915-24.5992
90.80810.1947-0.14140.4958-0.23974.82640.04910.08750.072-0.01980.05750.0738-0.0766-0.0722-0.08730.10240.0197-0.00760.09820.02740.120599.776713.1585-8.6241
101.38180.2670.81891.17870.53971.74930.0399-0.07020.24580.0268-0.05120.0987-0.1546-0.23650.01260.08820.02770.03120.12580.03560.1541109.17626.4243-8.5412
113.5554-1.2749-0.40334.0637-2.44691.9056-0.0835-0.41950.08510.33840.0014-0.1396-0.4407-0.04280.12770.16140.02730.02820.10360.01860.0981118.540823.4719-1.5508
123.20380.8674-0.61450.2356-0.11731.77640.10920.21380.6111-0.0759-0.018-0.080.0812-0.1409-0.08190.17140.02620.04620.18130.10950.1971126.96929.3744-27.3607
131.19370.2522-0.98391.7404-0.3971.27570.15630.4120.3208-0.2828-0.0663-0.23170.0073-0.1197-0.03940.19850.03450.07640.20540.08190.239134.81626.515-32.4459
144.7702-2.4264-1.59322.23451.28665.07820.1344-0.0305-0.1438-0.04660.079-0.50270.28660.5202-0.10250.15440.04860.09150.1505-0.01710.5478153.919821.9793-23.7074
153.72851.62060.2345.0776-1.52182.8524-0.05640.19340.45770.02830.1284-0.2753-0.35990.1616-0.07290.1465-0.00110.04970.1350.02060.2086138.536134.6988-20.3101
161.2361-1.0586-0.28734.32580.47980.67420.07910.09820.0958-0.1554-0.0821-0.1644-0.0253-0.03110.01290.09960.00090.01170.11370.02460.0914133.858516.6316-13.121
170.92520.2883-0.03522.59190.90341.05840.08660.220.0537-0.1783-0.0735-0.08590.0232-0.1618-0.00230.12680.02090.02640.17730.04360.0966122.113614.7692-24.0012
182.5556-1.5087-0.23532.3867-2.58484.98650.20840.057-0.3453-0.2899-0.18480.20560.53460.1442-0.07670.16650.045-0.01970.17540.00750.0872115.1375.6827-19.5353
192.7065-0.3654-0.0210.75320.43081.6765-0.041-0.2291-0.42430.2399-0.0391-0.1532-0.00910.04670.08540.1223-0.0103-0.01660.15080.07170.2293135.8672-6.906812.4968
201.6914-0.36020.53121.23410.02531.3480.0558-0.3182-0.12970.3324-0.0699-0.3054-0.070.17640.02490.175-0.0225-0.07760.18770.04750.2025143.7757-2.240915.7149
216.09351.43551.02385.25473.58546.90610.27420.161-0.02280.04590.2132-0.6938-0.09111.1283-0.29370.1318-0.0154-0.0230.2665-0.00780.3143158.72436.19582.6269
221.4102-0.5214-0.09693.0217-1.11992.54560.0113-0.012-0.53290.0448-0.0579-0.20040.20760.26940.0190.12030.0027-0.05860.13730.02020.2569146.372-9.64332.6424
230.97820.6512-0.08264.00830.79860.56350.0218-0.06090.00890.144-0.0456-0.250.020.0030.02510.1016-0.0001-0.02450.10280.02580.1037136.21276.6692-2.6418
241.1583-0.8085-0.24222.17540.94171.32060.0036-0.1640.10820.15420.0373-0.3513-0.0738-0.0112-0.03050.13170.0042-0.04040.1430.01950.1412127.05136.169710.6897
253.8816-1.3051.07274.1486-2.31921.769-0.2202-0.07660.3140.4680.0546-0.0706-0.37320.09630.16270.1822-0.0293-0.02390.14710.01680.0896117.424613.23938.0928
260.9948-0.5013-0.45471.76770.24730.8289-0.0538-0.3443-0.25270.24530.04940.23680.2017-0.0433-0.06810.1811-0.03550.01260.26310.12320.210752.061719.190540.3116
270.96190.4146-0.00550.55250.08730.56210.0856-0.197-0.37130.08660.04990.31060.2769-0.1805-0.04880.1127-0.1658-0.04230.16850.18870.440444.215816.159735.5954
280.46920.44790.8811.5991.93392.67290.1772-0.2340.1413-0.1133-0.27140.75040.1872-0.64380.04690.1537-0.0611-0.09030.33070.09640.695729.096529.724128.3786
292.5849-0.17460.20111.20240.50922.6372-0.0745-0.6234-0.14680.3150.04680.59630.1891-0.40360.05550.1334-0.00510.05650.30510.07950.297241.635828.658343.5667
300.7954-0.046-0.00420.6262-0.00784.20740.0334-0.0762-0.06160.03670.03880.06850.076-0.1497-0.07040.0871-0.01830.00960.10050.02680.104251.706534.951127.58
311.4584-0.2943-0.92571.18790.78041.9103-0.01380.0802-0.2483-0.0342-0.05780.13870.1306-0.23580.0540.0967-0.0212-0.02840.11460.03470.161561.09421.714327.4906
322.58681.19470.53052.5714-2.20053.0689-0.16970.279-0.0394-0.32390.0901-0.14080.50080.04260.13190.1875-0.0232-0.00090.11350.02250.108570.536224.672220.5361
333.0922-1.09430.80281.39640.24480.6316-0.03910.2920.431-0.01120.1471-0.178-0.0540.0262-0.11990.1284-0.02020.00240.13960.06840.159160.904360.86186.2894
340.4208-0.43330.4741.3781-0.35480.9729-0.17190.10190.5684-0.02390.06480.0928-0.1434-0.02970.04250.1430.0073-0.03770.14950.08760.290152.954165.76749.3992
356.8001-2.7838-2.16286.60411.86815.83630.1758-0.04560.56070.2112-0.11090.54-0.323-0.77970.04450.1450.03190.0420.27260.03830.375334.018456.25313.1276
362.60480.7275-0.98652.3842-0.28584.2102-0.11940.63070.3904-0.4030.00280.3328-0.1366-0.20610.11470.16820.019-0.040.20580.04560.167249.548153.96620.5261
371.2144-0.1675-0.64160.66530.27433.1557-0.02710.03660.0656-0.02760.01760.09660.0364-0.05580.00350.1025-0.0021-0.00840.08120.02220.081654.068245.919818.2012
381.47680.52290.21181.50460.37772.15180.072-0.09510.27170.124-0.03160.0424-0.2044-0.1987-0.05530.08890.02250.00420.09980.01480.142565.736756.746920.7065
393.6171-0.67-0.12233.008-1.16394.7003-0.0177-0.2912-0.05720.4790.01950.0894-0.21280.25910.01050.14540.00380.01410.10810.01480.111572.694251.813729.5582
403.64871.071-0.50251.370.78382.4076-0.04920.16050.2351-0.1978-0.0742-0.3303-0.0748-0.02670.09540.11140.00540.02620.14550.06490.15387.158153.93425.0762
413.3586-2.69271.93855.40990.54082.59980.2230.39060.0599-0.3824-0.0534-0.20810.03270.4133-0.15410.1217-0.00940.07760.15990.03480.2697101.134147.51074.015
421.59370.83250.29341.3055-0.5954.8660.01570.34250.1279-0.24280.0114-0.0945-0.20340.3394-0.03470.17150.05350.10110.19450.04890.227196.281351.27142.7318
435.654-1.8307-1.38025.91562.63457.32060.2904-0.23780.0112-0.09460.0688-0.79390.15661.0251-0.30920.12950.02590.00230.2388-0.02590.3555110.635841.931216.2081
442.47341.04740.19921.6143-0.87251.971-0.00340.03380.515-0.0674-0.0456-0.3024-0.20850.1680.0380.1201-0.00730.04850.13140.03640.283798.368457.63916.2716
450.6367-0.57040.08074.48210.68520.52320.01330.0508-0.0538-0.1441-0.032-0.1613-0.0070.00660.01750.083-0.00510.02010.10560.02020.116888.1441.404221.5904
461.2531.05150.35432.91961.68421.3761-0.00730.1751-0.1643-0.15750.0563-0.36260.0434-0.0384-0.03460.1402-0.00370.03690.14960.02250.150579.469139.41766.6872
471.37711.31810.65154.08431.21611.24140.02190.0229-0.00550.06060.065-0.02040.10540.0131-0.08840.11230.0240.01180.14960.02080.101871.826842.637613.0158
481.1401-0.5765-0.36290.5526-0.25460.91340.0132-0.1573-0.39160.05860.01620.08690.0121-0.1126-0.01870.1368-0.03-0.05360.16790.11320.275278.868118.723246.3034
491.0523-0.13240.34151.5544-0.03340.81520.1488-0.329-0.17260.2366-0.0015-0.29870.029-0.0445-0.00150.1839-0.036-0.09020.18370.09730.178286.731321.569951.3843
503.55142.53571.25612.63710.93485.62090.0260.10680.08930.06510.087-0.4617-0.39010.4882-0.08650.1484-0.0097-0.08140.14020.00530.5321105.822826.092342.6122
513.318-1.6569-0.85214.2981-0.94552.6846-0.0411-0.1561-0.4719-0.02690.1528-0.07790.22740.2063-0.10620.12950.0033-0.05260.14290.03690.252590.435413.456239.3015
521.16461.12770.12234.10440.39840.66030.0648-0.1006-0.11890.1278-0.0808-0.10520.0219-0.03890.01220.09480.0029-0.01980.10090.02570.100785.784231.475232.045
530.92810.0020.04413.08480.96340.36910.1117-0.1735-0.03610.199-0.1047-0.05510.0057-0.11520.00330.138-0.0225-0.02570.1610.0460.098773.97133.093942.8887
543.51081.19120.79562.4846-2.96825.22520.087-0.13520.4150.2863-0.1360.098-0.31060.16610.00930.1439-0.03820.00890.13420.0120.081867.084242.39338.4662
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resseq 2:84)A2 - 84
2X-RAY DIFFRACTION2chain 'A' and (resseq 85:125)A85 - 125
3X-RAY DIFFRACTION3chain 'A' and (resseq 126:214)A126 - 214
4X-RAY DIFFRACTION4chain 'A' and (resseq 215:295)A215 - 295
5X-RAY DIFFRACTION5chain 'B' and (resseq 2:21)B2 - 21
6X-RAY DIFFRACTION6chain 'B' and (resseq 22:84)B22 - 84
7X-RAY DIFFRACTION7chain 'B' and (resseq 85:105)B85 - 105
8X-RAY DIFFRACTION8chain 'B' and (resseq 106:125)B106 - 125
9X-RAY DIFFRACTION9chain 'B' and (resseq 126:214)B126 - 214
10X-RAY DIFFRACTION10chain 'B' and (resseq 215:277)B215 - 277
11X-RAY DIFFRACTION11chain 'B' and (resseq 278:295)B278 - 295
12X-RAY DIFFRACTION12chain 'C' and (resseq 2:21)C2 - 21
13X-RAY DIFFRACTION13chain 'C' and (resseq 22:84)C22 - 84
14X-RAY DIFFRACTION14chain 'C' and (resseq 85:105)C85 - 105
15X-RAY DIFFRACTION15chain 'C' and (resseq 106:125)C106 - 125
16X-RAY DIFFRACTION16chain 'C' and (resseq 126:214)C126 - 214
17X-RAY DIFFRACTION17chain 'C' and (resseq 215:277)C215 - 277
18X-RAY DIFFRACTION18chain 'C' and (resseq 278:295)C278 - 295
19X-RAY DIFFRACTION19chain 'D' and (resseq 2:21)D2 - 21
20X-RAY DIFFRACTION20chain 'D' and (resseq 22:84)D22 - 84
21X-RAY DIFFRACTION21chain 'D' and (resseq 85:105)D85 - 105
22X-RAY DIFFRACTION22chain 'D' and (resseq 106:125)D106 - 125
23X-RAY DIFFRACTION23chain 'D' and (resseq 126:214)D126 - 214
24X-RAY DIFFRACTION24chain 'D' and (resseq 215:277)D215 - 277
25X-RAY DIFFRACTION25chain 'D' and (resseq 278:295)D278 - 295
26X-RAY DIFFRACTION26chain 'E' and (resseq 2:21)E2 - 21
27X-RAY DIFFRACTION27chain 'E' and (resseq 22:84)E22 - 84
28X-RAY DIFFRACTION28chain 'E' and (resseq 85:105)E85 - 105
29X-RAY DIFFRACTION29chain 'E' and (resseq 106:125)E106 - 125
30X-RAY DIFFRACTION30chain 'E' and (resseq 126:214)E126 - 214
31X-RAY DIFFRACTION31chain 'E' and (resseq 215:277)E215 - 277
32X-RAY DIFFRACTION32chain 'E' and (resseq 278:295)E278 - 295
33X-RAY DIFFRACTION33chain 'F' and (resseq 2:21)F2 - 21
34X-RAY DIFFRACTION34chain 'F' and (resseq 22:84)F22 - 84
35X-RAY DIFFRACTION35chain 'F' and (resseq 85:105)F85 - 105
36X-RAY DIFFRACTION36chain 'F' and (resseq 106:125)F106 - 125
37X-RAY DIFFRACTION37chain 'F' and (resseq 126:214)F126 - 214
38X-RAY DIFFRACTION38chain 'F' and (resseq 215:277)F215 - 277
39X-RAY DIFFRACTION39chain 'F' and (resseq 278:295)F278 - 295
40X-RAY DIFFRACTION40chain 'G' and (resseq 2:35)G2 - 35
41X-RAY DIFFRACTION41chain 'G' and (resseq 36:54)G36 - 54
42X-RAY DIFFRACTION42chain 'G' and (resseq 55:84)G55 - 84
43X-RAY DIFFRACTION43chain 'G' and (resseq 85:105)G85 - 105
44X-RAY DIFFRACTION44chain 'G' and (resseq 106:125)G106 - 125
45X-RAY DIFFRACTION45chain 'G' and (resseq 126:214)G126 - 214
46X-RAY DIFFRACTION46chain 'G' and (resseq 215:266)G215 - 266
47X-RAY DIFFRACTION47chain 'G' and (resseq 267:295)G267 - 295
48X-RAY DIFFRACTION48chain 'H' and (resseq 2:21)H2 - 21
49X-RAY DIFFRACTION49chain 'H' and (resseq 22:84)H22 - 84
50X-RAY DIFFRACTION50chain 'H' and (resseq 85:105)H85 - 105
51X-RAY DIFFRACTION51chain 'H' and (resseq 106:125)H106 - 125
52X-RAY DIFFRACTION52chain 'H' and (resseq 126:214)H126 - 214
53X-RAY DIFFRACTION53chain 'H' and (resseq 215:277)H215 - 277
54X-RAY DIFFRACTION54chain 'H' and (resseq 278:295)H278 - 295

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