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- PDB-4pm5: Crystal structure of CTX-M-14 S70G beta-lactamase in complex with... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4pm5 | |||||||||
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Title | Crystal structure of CTX-M-14 S70G beta-lactamase in complex with cefotaxime at 1.26 Angstroms resolution | |||||||||
![]() | Beta-lactamase CTX-M-14 | |||||||||
![]() | HYDROLASE/ANTIBIOTIC / Class A beta-lactamase / cefotaxime / HYDROLASE-ANTIBIOTIC complex | |||||||||
Function / homology | ![]() beta-lactam antibiotic catabolic process / beta-lactamase activity / beta-lactamase / response to antibiotic Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Adamski, C.J. / Cardenas, A.M. / Sankaran, B. / Palzkill, T. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular Basis for the Catalytic Specificity of the CTX-M Extended-Spectrum beta-Lactamases. Authors: Adamski, C.J. / Cardenas, A.M. / Brown, N.G. / Horton, L.B. / Sankaran, B. / Prasad, B.V. / Gilbert, H.F. / Palzkill, T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 76 KB | Display | ![]() |
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PDB format | ![]() | 53 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 814.4 KB | Display | ![]() |
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Full document | ![]() | 817.7 KB | Display | |
Data in XML | ![]() | 16.6 KB | Display | |
Data in CIF | ![]() | 25.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4pm6C ![]() 4pm7C ![]() 4pm8C ![]() 4pm9C ![]() 4pmaC ![]() 1yltS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27970.520 Da / Num. of mol.: 1 / Fragment: UNP residues 29-291 / Mutation: S70G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: HS11286 / Gene: KPHS_p301310 / Production host: ![]() ![]() | ||
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#2: Chemical | ChemComp-CE3 / ( | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38.65 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / Details: Ammonium phosphate, PEG 4000 |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 4, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.26→29.5 Å / Num. obs: 61202 / % possible obs: 99.7 % / Redundancy: 2 % / Net I/σ(I): 19.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1YLT Resolution: 1.261→29.451 Å / SU ML: 0.1 / Cross valid method: NONE / σ(F): 1.93 / Phase error: 16.45 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.261→29.451 Å
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Refine LS restraints |
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LS refinement shell |
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