ジャーナル: J Mol Biol / 年: 2014 タイトル: KIF14 binds tightly to microtubules and adopts a rigor-like conformation. 著者: Kritica Arora / Lama Talje / Ana B Asenjo / Parker Andersen / Kaleem Atchia / Monika Joshi / Hernando Sosa / John S Allingham / Benjamin H Kwok / 要旨: The mitotic kinesin motor protein KIF14 is essential for cytokinesis during cell division and has been implicated in cerebral development and a variety of human cancers. Here we show that the mouse ...The mitotic kinesin motor protein KIF14 is essential for cytokinesis during cell division and has been implicated in cerebral development and a variety of human cancers. Here we show that the mouse KIF14 motor domain binds tightly to microtubules and does not display typical nucleotide-dependent changes in this affinity. It also has robust ATPase activity but very slow motility. A crystal structure of the ADP-bound form of the KIF14 motor domain reveals a dramatically opened ATP-binding pocket, as if ready to exchange its bound ADP for Mg·ATP. In this state, the central β-sheet is twisted ~10° beyond the maximal amount observed in other kinesins. This configuration has only been seen in the nucleotide-free states of myosins-known as the "rigor-like" state. Fitting of this atomic model to electron density maps from cryo-electron microscopy indicates a distinct binding configuration of the motor domain to microtubules. We postulate that these properties of KIF14 are well suited for stabilizing midbody microtubules during cytokinesis.
A: Chimera of Maltose-binding periplasmic protein and Kinesin family member 14 protein B: Chimera of Maltose-binding periplasmic protein and Kinesin family member 14 protein ヘテロ分子
解像度: 2.71→30 Å / Num. obs: 40225 / % possible obs: 94.6 % / 冗長度: 3.1 % / Net I/σ(I): 10.72
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解析
ソフトウェア
名称: REFMAC / バージョン: 5.7.0032 / 分類: 精密化
精密化
解像度: 2.71→29.36 Å / Cor.coef. Fo:Fc: 0.883 / Cor.coef. Fo:Fc free: 0.829 / SU B: 39.339 / SU ML: 0.387 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R Free: 0.456 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
Rfactor
反射数
%反射
Selection details
Rfree
0.3075
2034
5.1 %
RANDOM
Rwork
0.2638
38158
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obs
0.2661
40192
89.5 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK