Mass: 18.015 Da / Num. of mol.: 111 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.97 Å3/Da / Density % sol: 37.52 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.4 Details: crystal of lysozyme have been obtained in the presence of Aubipyc (1:10 protein to metal ratio) via the hanging drop vapor diffusion method. These crystals have been grown using a reservoir ...Details: crystal of lysozyme have been obtained in the presence of Aubipyc (1:10 protein to metal ratio) via the hanging drop vapor diffusion method. These crystals have been grown using a reservoir solution of 0.6 M NaNO3, 0.1 M sodium acetate pH 4.4 and 20 % ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution: 1.8→25.55 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.935 / SU B: 3.933 / SU ML: 0.119 / Cross valid method: THROUGHOUT / ESU R: 0.167 / ESU R Free: 0.16 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.24378
499
4.8 %
RANDOM
Rwork
0.18075
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obs
0.18383
9988
94.44 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK