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- PDB-4oit: Structure, interactions and evolutionary implications of a domain... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4oit | ||||||
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Title | Structure, interactions and evolutionary implications of a domain-swapped lectin dimer from Mycobacterium smegmatis | ||||||
![]() | LysM domain protein | ||||||
![]() | SUGAR BINDING PROTEIN / Beta-prism II fold / bacterial lectin / protein-carbohydrate interactions / Beta-Prism II / Carbohydrate binding / Carbohydrate/Sugar | ||||||
Function / homology | ![]() Agglutinin, subunit A / Bulb-type lectin domain / Bulb-type lectin domain / Bulb-type lectin domain superfamily / Bulb-type lectin domain profile. / Bulb-type mannose-specific lectin / Orthogonal Prism / Lysin motif / LysM domain superfamily / LysM domain ...Agglutinin, subunit A / Bulb-type lectin domain / Bulb-type lectin domain / Bulb-type lectin domain superfamily / Bulb-type lectin domain profile. / Bulb-type mannose-specific lectin / Orthogonal Prism / Lysin motif / LysM domain superfamily / LysM domain / LysM domain profile. / LysM domain / Mainly Beta Similarity search - Domain/homology | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Patra, D. / Mishra, P. / Surolia, A. / Vijayan, M. | ||||||
![]() | ![]() Title: Structure, interactions and evolutionary implications of a domain-swapped lectin dimer from Mycobacterium smegmatis. Authors: Patra, D. / Mishra, P. / Surolia, A. / Vijayan, M. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2011 Title: Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis Authors: Patra, D. / Sharma, A. / Chandran, D. / Vijayan, M. #2: Journal: J.Biosci. / Year: 2007 Title: Multiplicity of carbohydrate-binding sites in beta-prism fold lectins: occurrence and possible evolutionary implications Authors: Sharma, A. / Chandran, D. / Singh, D.D. / Vijayan, M. #3: Journal: Proteins / Year: 2013 Title: Identification of mycobacterial lectins from genomic data Authors: Abhinav, K.V. / Sharma, A. / Vijayan, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95.2 KB | Display | ![]() |
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PDB format | ![]() | 74 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12541.867 Da / Num. of mol.: 4 / Fragment: mannose-binding lectin domain, UNP residues 1-105 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Sugar | ChemComp-MAN / #3: Sugar | ChemComp-BMA / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.11 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.2M tri-sodium citrate, 0.1M Na HEPES, 6% glycerol, 30%(w/v) 1,5 diammino pentane dihydrochloride, 60mM mannose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Mar 11, 2012 / Details: mirrors |
Radiation | Monochromator: Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54179 Å / Relative weight: 1 |
Reflection | Resolution: 2.24→40 Å / Num. all: 47027 / Num. obs: 19379 / % possible obs: 98.7 % / Redundancy: 2.7 % / Rmerge(I) obs: 0.112 / Net I/σ(I): 6.2 |
Reflection shell | Resolution: 2.24→2.36 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.38 / Num. unique all: 2766 / % possible all: 97.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: Native structure Resolution: 2.24→40 Å / Cor.coef. Fo:Fc: 0.915 / Cor.coef. Fo:Fc free: 0.882 / SU B: 7.79 / SU ML: 0.197 / Cross valid method: THROUGHOUT / ESU R: 0.44 / ESU R Free: 0.259 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.834 Å2
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Refinement step | Cycle: LAST / Resolution: 2.24→40 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.24→2.298 Å / Total num. of bins used: 20
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