Entry Database : PDB / ID : 4o9r Structure visualization Downloads & linksTitle Human Smoothened Receptor structure in complex with cyclopamine ComponentsSmoothened homolog/Soluble cytochrome b562 chimeric protein Details Keywords SIGNALING PROTEIN / serial femtosecond crystallography / human SMO receptor / cyclopamine / novel protein engineering / GPCR network / PSI-Biology / free electron laser / Structural Genomics / membrane protein / XFEL / LCP / room temperature structure / GPCR / membraneFunction / homology Function and homology informationFunction Domain/homology Component
ventral midline determination / mesenchymal to epithelial transition involved in metanephric renal vesicle formation / regulation of heart morphogenesis / contact inhibition / negative regulation of hair follicle development / central nervous system neuron differentiation / 9+0 non-motile cilium / pancreas morphogenesis / regulation of somatic stem cell population maintenance / epithelial-mesenchymal cell signaling ... ventral midline determination / mesenchymal to epithelial transition involved in metanephric renal vesicle formation / regulation of heart morphogenesis / contact inhibition / negative regulation of hair follicle development / central nervous system neuron differentiation / 9+0 non-motile cilium / pancreas morphogenesis / regulation of somatic stem cell population maintenance / epithelial-mesenchymal cell signaling / myoblast migration / atrial septum morphogenesis / determination of left/right asymmetry in lateral mesoderm / spinal cord dorsal/ventral patterning / left/right axis specification / ciliary tip / Activation of SMO / thalamus development / somite development / patched binding / forebrain morphogenesis / cellular response to cholesterol / type B pancreatic cell development / dorsal/ventral neural tube patterning / positive regulation of branching involved in ureteric bud morphogenesis / positive regulation of organ growth / BBSome-mediated cargo-targeting to cilium / smooth muscle tissue development / pattern specification process / cerebellar cortex morphogenesis / mammary gland epithelial cell differentiation / dentate gyrus development / positive regulation of multicellular organism growth / dopaminergic neuron differentiation / commissural neuron axon guidance / oxysterol binding / positive regulation of smoothened signaling pathway / Class B/2 (Secretin family receptors) / cell fate specification / neural crest cell migration / cAMP-dependent protein kinase inhibitor activity / ciliary membrane / anterior/posterior pattern specification / positive regulation of mesenchymal cell proliferation / negative regulation of epithelial cell differentiation / midgut development / smoothened signaling pathway / hair follicle morphogenesis / positive regulation of neuroblast proliferation / heart looping / negative regulation of DNA binding / odontogenesis of dentin-containing tooth / protein kinase A catalytic subunit binding / endoplasmic reticulum-Golgi intermediate compartment / neuroblast proliferation / vasculogenesis / Hedgehog 'off' state / skeletal muscle fiber development / homeostasis of number of cells within a tissue / protein sequestering activity / centriole / negative regulation of protein phosphorylation / epithelial cell proliferation / central nervous system development / positive regulation of epithelial cell proliferation / astrocyte activation / G protein-coupled receptor activity / electron transport chain / Hedgehog 'on' state / multicellular organism growth / cerebral cortex development / cilium / positive regulation of protein import into nucleus / osteoblast differentiation / protein import into nucleus / endocytic vesicle membrane / late endosome / gene expression / in utero embryonic development / periplasmic space / electron transfer activity / protein stabilization / positive regulation of cell migration / iron ion binding / negative regulation of gene expression / intracellular membrane-bounded organelle / apoptotic process / dendrite / heme binding / positive regulation of gene expression / negative regulation of apoptotic process / Golgi apparatus / negative regulation of transcription by RNA polymerase II / endoplasmic reticulum / positive regulation of transcription by RNA polymerase II / extracellular exosome / plasma membrane Similarity search - Function Smoothened, transmembrane domain / Smoothened, cysteine-rich domain / Frizzled/Smoothened, transmembrane domain / Frizzled/Smoothened family membrane region / Frizzled/Smoothened family membrane region / Frizzled/secreted frizzled-related protein / Frizzled / Frizzled domain / Frizzled cysteine-rich domain superfamily / Fz domain ... Smoothened, transmembrane domain / Smoothened, cysteine-rich domain / Frizzled/Smoothened, transmembrane domain / Frizzled/Smoothened family membrane region / Frizzled/Smoothened family membrane region / Frizzled/secreted frizzled-related protein / Frizzled / Frizzled domain / Frizzled cysteine-rich domain superfamily / Fz domain / Frizzled (fz) domain profile. / GPCR, family 2-like / G-protein coupled receptors family 2 profile 2. / Cytochrome b562 / Cytochrome b562 / Cytochrome c/b562 Similarity search - Domain/homologyBiological species Homo sapiens (human)Escherichia coli (E. coli)Method X-RAY DIFFRACTION / FREE ELECTRON LASER / MOLECULAR REPLACEMENT / Resolution : 3.204 Å DetailsAuthors Wang, C. / Weierstall, U. / James, D. / White, T.A. / Wang, D. / Liu, W. / Spence, J.C.H. / Doak, R.B. / Nelson, G. / Fromme, P. ...Wang, C. / Weierstall, U. / James, D. / White, T.A. / Wang, D. / Liu, W. / Spence, J.C.H. / Doak, R.B. / Nelson, G. / Fromme, P. / Fromme, R. / Grotjohann, I. / Kupitz, C. / Zatsepin, N.A. / Liu, H. / Basu, S. / Wacker, D. / Han, G.W. / Katritch, V. / Boutet, S. / Messerschmidt, M. / Willams, G.J. / Koglin, J.E. / Seibert, M.M. / Klinker, M. / Gati, C. / Shoeman, R.L. / Barty, A. / Chapman, H.N. / Kirian, R.A. / Beyerlein, K.R. / Stevens, R.C. / Li, D. / Shah, S.T.A. / Howe, N. / Caffrey, M. / Cherezov, V. / GPCR Network (GPCR) CitationJournal : Nat Commun / Year : 2014Title : Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.Authors: Weierstall, U. / James, D. / Wang, C. / White, T.A. / Wang, D. / Liu, W. / Spence, J.C. / Bruce Doak, R. / Nelson, G. / Fromme, P. / Fromme, R. / Grotjohann, I. / Kupitz, C. / Zatsepin, N.A. ... Authors : Weierstall, U. / James, D. / Wang, C. / White, T.A. / Wang, D. / Liu, W. / Spence, J.C. / Bruce Doak, R. / Nelson, G. / Fromme, P. / Fromme, R. / Grotjohann, I. / Kupitz, C. / Zatsepin, N.A. / Liu, H. / Basu, S. / Wacker, D. / Han, G.W. / Katritch, V. / Boutet, S. / Messerschmidt, M. / Williams, G.J. / Koglin, J.E. / Marvin Seibert, M. / Klinker, M. / Gati, C. / Shoeman, R.L. / Barty, A. / Chapman, H.N. / Kirian, R.A. / Beyerlein, K.R. / Stevens, R.C. / Li, D. / Shah, S.T. / Howe, N. / Caffrey, M. / Cherezov, V. History Deposition Jan 2, 2014 Deposition site : RCSB / Processing site : RCSBRevision 1.0 Mar 5, 2014 Provider : repository / Type : Initial releaseRevision 1.1 Aug 2, 2017 Group : Refinement description / Source and taxonomy / Category : entity_src_gen / softwareRevision 1.2 Feb 14, 2018 Group : Data collection / Category : diffrn_sourceItem : _diffrn_source.pdbx_synchrotron_beamline / _diffrn_source.pdbx_synchrotron_siteRevision 1.3 Aug 16, 2023 Group : Data collection / Database references / Derived calculationsCategory : chem_comp_atom / chem_comp_bond ... chem_comp_atom / chem_comp_bond / database_2 / pdbx_related_exp_data_set / struct_ref_seq_dif / struct_site Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession ... _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id Revision 1.4 Sep 20, 2023 Group : Refinement description / Category : pdbx_initial_refinement_model
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