+Open data
-Basic information
Entry | Database: PDB / ID: 4o6g | ||||||
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Title | Rv3902c from M. tuberculosis | ||||||
Components | Uncharacterized protein | ||||||
Keywords | UNKNOWN FUNCTION / Structural Genomics / NIAID / National Institute of Allergy and Infectious Diseases / TB Structural Genomics Consortium / TBSGC | ||||||
Function / homology | Immunity protein IFT-like / Immunity protein 61 / Uncharacterized protein Function and homology information | ||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.55 Å | ||||||
Authors | Reddy, B.G. / Moates, D.B. / Kim, H. / Green, T.J. / Kim, C. / Terwilliger, T.J. / Delucas, L.J. / TB Structural Genomics Consortium (TBSGC) | ||||||
Citation | Journal: Acta Crystallogr F Struct Biol Commun / Year: 2014 Title: 1.55 angstrom resolution X-ray crystal structure of Rv3902c from Mycobacterium tuberculosis. Authors: Reddy, B.G. / Moates, D.B. / Kim, H.B. / Green, T.J. / Kim, C.Y. / Terwilliger, T.C. / Delucas, L.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4o6g.cif.gz | 86.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4o6g.ent.gz | 66.9 KB | Display | PDB format |
PDBx/mmJSON format | 4o6g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4o6g_validation.pdf.gz | 400 KB | Display | wwPDB validaton report |
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Full document | 4o6g_full_validation.pdf.gz | 400.6 KB | Display | |
Data in XML | 4o6g_validation.xml.gz | 10.7 KB | Display | |
Data in CIF | 4o6g_validation.cif.gz | 15.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/o6/4o6g ftp://data.pdbj.org/pub/pdb/validation_reports/o6/4o6g | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19546.965 Da / Num. of mol.: 1 / Fragment: Rv3902c Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Strain: ATCC BAA-935 / AF2122/97 / Gene: Mb3932c, MT4020, Rv3902c / Production host: Escherichia coli (E. coli) / References: UniProt: L7N519 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.38 Å3/Da / Density % sol: 63.57 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: Protein Concentration 25mg/ml, 1.5M ammonium sulphate, and 200mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å |
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Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Apr 20, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→39.76 Å / Num. all: 37938 / Num. obs: 37938 / % possible obs: 100 % / Observed criterion σ(I): 2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Bromide Soaked SAD Crystal Structure Resolution: 1.55→39.759 Å / SU ML: 0.13 / σ(F): 1.36 / Phase error: 16.84 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.55→39.759 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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