+Open data
-Basic information
Entry | Database: PDB / ID: 4mo0 | ||||||
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Title | Crystal structure of aIF1 from Methanocaldococcus jannaschii | ||||||
Components | Protein translation factor SUI1 homolog | ||||||
Keywords | TRANSLATION / alpha-beta domain | ||||||
Function / homology | Function and homology information translation reinitiation / formation of translation preinitiation complex / translation initiation factor activity / mRNA binding Similarity search - Function | ||||||
Biological species | Methanocaldococcus jannaschii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SIRAS / Resolution: 2.1 Å | ||||||
Authors | Schmitt, E. / Mechulam, Y. | ||||||
Citation | Journal: To be Published Title: Crystal structure of aIF1 from Methanocaldococcus jannaschii Authors: Coureux, P.-D. / Schmitt, E. / Monestier, A. / Larquet, E. / Cladiere, L. / Menetret, J.-F. / Klaholz, B. / Mechulam, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4mo0.cif.gz | 42 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4mo0.ent.gz | 32.8 KB | Display | PDB format |
PDBx/mmJSON format | 4mo0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mo/4mo0 ftp://data.pdbj.org/pub/pdb/validation_reports/mo/4mo0 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 11621.787 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanocaldococcus jannaschii (archaea) Gene: MJ0463, sui1 / Plasmid: pet15blpa / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 Rosetta / References: UniProt: Q57902 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.75 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 25% PEG3350, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.98011 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 16, 2010 |
Radiation | Monochromator: channel cut Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98011 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→32.9 Å / Num. all: 7257 / Num. obs: 7272 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 18.7 % / Biso Wilson estimate: 38.7 Å2 / Rmerge(I) obs: 0.063 / Rsym value: 0.032 |
Reflection shell | Resolution: 2.1→2.2 Å / Redundancy: 17.9 % / Rmerge(I) obs: 0.595 / Mean I/σ(I) obs: 5.5 / Num. unique all: 905 / Rsym value: 0.578 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: SIRAS / Resolution: 2.1→32.866 Å / SU ML: 0.44 / σ(F): 1.38 / Phase error: 23.22 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 49.946 Å2 / ksol: 0.37 e/Å3 | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.54 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→32.866 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 1.5593 Å / Origin y: 14.6822 Å / Origin z: 6.4597 Å
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Refinement TLS group | Selection details: all |