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Yorodumi- PDB-4mnp: Structure of the Sialic Acid Binding Protein from Fusobacterium N... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4mnp | ||||||
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Title | Structure of the Sialic Acid Binding Protein from Fusobacterium Nucleatum subsp. nucleatum ATCC 25586 | ||||||
Components | N-acetylneuraminate-binding protein | ||||||
Keywords | SUGAR BINDING PROTEIN / Sialic Acid binding protein / Sialic Acid | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Fusobacterium nucleatum subsp. nucleatum (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.5 Å | ||||||
Authors | Thanuja, G. / Ramaswamy, S. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2014 Title: Bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site. Authors: Gangi Setty, T. / Cho, C. / Govindappa, S. / Apicella, M.A. / Ramaswamy, S. #1: Journal: J.Biol.Chem. / Year: 2008 Title: Characterization of the N-acetyl-5-neuraminic acid-binding site of the extracytoplasmic solute receptor (SiaP) of nontypeable Haemophilus influenzae strain 2019. Authors: Johnston, J.W. / Coussens, N.P. / Allen, S. / Houtman, J.C. / Turner, K.H. / Zaleski, A. / Ramaswamy, S. / Gibson, B.W. / Apicella, M.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4mnp.cif.gz | 75 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4mnp.ent.gz | 54.9 KB | Display | PDB format |
PDBx/mmJSON format | 4mnp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mn/4mnp ftp://data.pdbj.org/pub/pdb/validation_reports/mn/4mnp | HTTPS FTP |
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-Related structure data
Related structure data | 4magC 4mmpC 3b50S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35372.641 Da / Num. of mol.: 1 / Fragment: UNP residues 24-327 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Fusobacterium nucleatum subsp. nucleatum (bacteria) Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 / Gene: FN1472, SiaP / Plasmid: pET28A / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8RDN9 |
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#2: Sugar | ChemComp-SLB / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38.5 % |
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Crystal grow | Temperature: 277 K / pH: 8.5 Details: Drops were setup with equal volume of protein and 0.2M MgCl2, 0.1M TRIS 8.5, 35% PEG 4000 (crystallization buffer) and suspended over 100ul of crystallization buffer., VAPOR DIFFUSION, ...Details: Drops were setup with equal volume of protein and 0.2M MgCl2, 0.1M TRIS 8.5, 35% PEG 4000 (crystallization buffer) and suspended over 100ul of crystallization buffer., VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Nov 2, 2012 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.056→41.84 Å / Num. obs: 17627 / % possible obs: 98.9 % / Observed criterion σ(I): 0 / Redundancy: 6.1 % / Rmerge(I) obs: 0.116 / Net I/σ(I): 9.6 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3B50 Resolution: 2.5→40.47 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 0.29 / σ(F): 1.19 / Phase error: 22.51 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.13 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→40.47 Å
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Refine LS restraints |
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LS refinement shell |
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