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Open data
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Basic information
Entry | Database: PDB / ID: 4mjj | ||||||
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Title | Crystal structure of the C2A domain of DOC2A | ||||||
![]() | Double C2-like domain-containing protein alpha | ||||||
![]() | METAL BINDING PROTEIN / C2 domain / DOC2A / calcium binding protein | ||||||
Function / homology | ![]() spontaneous neurotransmitter secretion / regulation of calcium ion-dependent exocytosis / extrinsic component of synaptic vesicle membrane / calcium-dependent phospholipid binding / synaptic vesicle exocytosis / cell junction / nervous system development / chemical synaptic transmission / lysosome / neuron projection ...spontaneous neurotransmitter secretion / regulation of calcium ion-dependent exocytosis / extrinsic component of synaptic vesicle membrane / calcium-dependent phospholipid binding / synaptic vesicle exocytosis / cell junction / nervous system development / chemical synaptic transmission / lysosome / neuron projection / calcium ion binding / nucleolus / nucleoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, Q.Q. | ||||||
![]() | ![]() Title: Crystal structure of the C2A domain of DOC2A Authors: Huang, Q.Q. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 34.1 KB | Display | ![]() |
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PDB format | ![]() | 25.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 15651.939 Da / Num. of mol.: 1 / Fragment: C2A, unp residues 81-217 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.18 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 14% PEG400, 100mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: May 25, 2013 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.918 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→35 Å / Num. all: 15800 / Num. obs: 15658 / % possible obs: 99.1 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3.6 % / Rmerge(I) obs: 0.136 |
Reflection shell | Resolution: 1.7→1.73 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.275 / Num. unique all: 760 / % possible all: 96.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→21.215 Å
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Refine LS restraints |
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LS refinement shell |
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