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- PDB-4m2b: Crystal structure of L281D mutant of udp-glucose pyrophosphorylas... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4m2b | ||||||
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Title | Crystal structure of L281D mutant of udp-glucose pyrophosphorylase from leishmania major in complex with udp-glc | ||||||
![]() | UDP-glucose pyrophosphorylase | ||||||
![]() | TRANSFERASE | ||||||
Function / homology | ![]() UTP-glucose-1-phosphate uridylyltransferase / UTP:glucose-1-phosphate uridylyltransferase activity / UDP-glucose metabolic process / nuclear lumen / ciliary plasm / glycogen metabolic process / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fuehring, J. / Routier, F.H. / Lamerz, A.-C. / Baruch, P. / Gerardy-Schahn, R. / Fedorov, R. | ||||||
![]() | ![]() Title: Catalytic Mechanism and Allosteric Regulation of Udp-Glucose Pyrophosphorylase from Leishmania Major Authors: Fuehring, J. / Routier, F.H. / Lamerz, A.-C. / Baruch, P. / Gerardy-Schahn, R. / Fedorov, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.8 KB | Display | ![]() |
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PDB format | ![]() | 88 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 801.5 KB | Display | ![]() |
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Full document | ![]() | 815.8 KB | Display | |
Data in XML | ![]() | 24.8 KB | Display | |
Data in CIF | ![]() | 36.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4m2aC ![]() 2oegS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 56056.699 Da / Num. of mol.: 1 / Mutation: L281D Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q4QDU3, UTP-glucose-1-phosphate uridylyltransferase |
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#2: Chemical | ChemComp-UPG / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.72 % |
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Crystal grow | Temperature: 277 K / pH: 6.6 Details: 0.1M BIS-TRIS, 28% W/V PEG-MME -2000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K, pH 6.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Mar 25, 2006 |
Radiation | Monochromator: A SINGLE GE(111) TRIANGULAR BENT CRYSTAL. / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.816 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→23.4 Å / Num. obs: 23985 / % possible obs: 98 % / Observed criterion σ(I): 0 / Redundancy: 8.7 % / Biso Wilson estimate: 31 Å2 / Rmerge(I) obs: 0.158 / Rsym value: 0.076 / Net I/σ(I): 14.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2OEG Resolution: 2.2→23.4 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 29.14 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→23.4 Å
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