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Yorodumi- PDB-4m2a: Crystal structure of the udp-glucose pyrophosphorylase from Leish... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4m2a | ||||||
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Title | Crystal structure of the udp-glucose pyrophosphorylase from Leishmania major in the post-reactive state | ||||||
Components | UDP-glucose pyrophosphorylaseUTP—glucose-1-phosphate uridylyltransferase | ||||||
Keywords | TRANSFERASE / ROSSMANN-LIKE ALPHA/BETA/ALPHA SANDWICH FOLD / NUCLEOTIDYLTRANSFERASE | ||||||
Function / homology | Function and homology information UTP-glucose-1-phosphate uridylyltransferase / UTP:glucose-1-phosphate uridylyltransferase activity / UDP-glucose metabolic process / nuclear lumen / ciliary plasm / glycogen metabolic process / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Leishmania major (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.66 Å | ||||||
Authors | Fuehring, J. / Routier, F.H. / Lamerz, A.-C. / Baruch, P. / Gerardy-Schahn, R. / Fedorov, R. | ||||||
Citation | Journal: ACS CATALYSIS / Year: 2013 Title: Catalytic Mechanism and Allosteric Regulation of Udp-Glucose Pyrophosphorylase from Leishmania Major Authors: Fuehring, J. / Routier, F.H. / Lamerz, A.-C. / Baruch, P. / Gerardy-Schahn, R. / Fedorov, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4m2a.cif.gz | 125.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4m2a.ent.gz | 94.1 KB | Display | PDB format |
PDBx/mmJSON format | 4m2a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m2/4m2a ftp://data.pdbj.org/pub/pdb/validation_reports/m2/4m2a | HTTPS FTP |
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-Related structure data
Related structure data | 4m2bC 2oegS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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Details | BIOMOLECULE: 1 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON BURIED SURFACE AREA. COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. BIOMOLECULE: 1 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC SOFTWARE USED: PISA APPLY THE FOLLOWING TO CHAINS: A BIOMT1 1 1.000000 0.000000 0.000000 0.00000 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 |
-Components
#1: Protein | Mass: 56054.770 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Leishmania major (eukaryote) / Gene: LMJF_18_0990, UGP / Production host: Escherichia coli (E. coli) References: UniProt: Q4QDU3, UTP-glucose-1-phosphate uridylyltransferase |
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#2: Chemical | ChemComp-UPG / |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-SO4 / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.15 % |
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6 Details: 0.1M BIS-TRIS, 22% W/V PEG-MME- 2000, 20MM (NH4)2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X13 / Wavelength: 0.806 / Wavelength: 0.8055 Å | |||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Oct 31, 2007 | |||||||||
Radiation | Monochromator: THE TRIANGULAR SHAPED GE (OR SI) MONOCHROMATOR CUT AT 7 FOR COMPRESSION OF THE BEAM IS 180 MM LONG, 1 MM THICK AND 40 MM IN HEIGHT AT THE BASE. Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.66→29.6 Å / Num. all: 57313 / Num. obs: 57313 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 / Redundancy: 14.93 % / Biso Wilson estimate: 26.4 Å2 / Rmerge(I) obs: 0.068 / Rsym value: 0.023 / Net I/σ(I): 28.78 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2OEG Resolution: 1.66→29.6 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.66→29.6 Å
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