Mass: 18.015 Da / Num. of mol.: 650 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.19 Å3/Da / Density % sol: 43.85 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: The protein was concentrated at 4mg/mL. The conditions are 0.2M sodium acetate, 0.1M sodium cacodylate, 25% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.5
Resolution: 1.3→36.1 Å / Cor.coef. Fo:Fc: 0.986 / Cor.coef. Fo:Fc free: 0.975 / SU B: 1.368 / SU ML: 0.026 / Cross valid method: THROUGHOUT / ESU R: 0.039 / ESU R Free: 0.04 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.14253
4123
5 %
RANDOM
Rwork
0.10687
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obs
0.10864
78327
99.91 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK