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Yorodumi- PDB-4lzu: Bovine beta-lactoglobulin crystallized in the presence of 2 mM zi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4lzu | ||||||
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Title | Bovine beta-lactoglobulin crystallized in the presence of 2 mM zinc chloride | ||||||
Components | Beta-lactoglobulin | ||||||
Keywords | TRANSPORT PROTEIN | ||||||
Function / homology | Function and homology information retinol binding / long-chain fatty acid binding / extracellular region / identical protein binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Oelker, M. / Zocher, G. / Stehle, T. | ||||||
Citation | Journal: To be Published Title: Non-Classical Pathways of Protein Crystallization in the Presence of Multivalent Metal Ions Authors: Sauter, A. / Oelker, M. / Zocher, G. / Zhang, F. / Stehle, T. / Schreiber, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4lzu.cif.gz | 77.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4lzu.ent.gz | 58.4 KB | Display | PDB format |
PDBx/mmJSON format | 4lzu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lz/4lzu ftp://data.pdbj.org/pub/pdb/validation_reports/lz/4lzu | HTTPS FTP |
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-Related structure data
Related structure data | 4lzvC 3ph5S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18301.174 Da / Num. of mol.: 1 / Fragment: UNP residues 17-178 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P02754 | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.23 % |
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Crystal grow | Temperature: 277 K / Method: batch / pH: 6 Details: 2 mM zinc chloride, pH 6.0, BATCH, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Nov 25, 2011 |
Radiation | Monochromator: Bartels Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→19.73 Å / Num. all: 8425 / Num. obs: 8425 / % possible obs: 99.8 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 11.3 % / Net I/σ(I): 29.62 |
Reflection shell | Resolution: 2.4→2.46 Å / Redundancy: 9.9 % / Mean I/σ(I) obs: 3.05 / % possible all: 99.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 3PH5 Resolution: 2.4→19.73 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.949 / SU B: 16.857 / SU ML: 0.183 / Cross valid method: THROUGHOUT / ESU R: 0.361 / ESU R Free: 0.24 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 74.463 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→19.73 Å
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Refine LS restraints |
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