温度: 298 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 5.5 詳細: A 0.6 uL drop of protein solution [15 mg/mL F95M EIZS, 20 mM Tris-HCl (pH 7.5), 300 mM NaCl, 10 mM MgCl2, 10% glycerol, 2 mM TCEP, 2 mM sodium pyrophosphate, 2 mM BTAC] was added to 0.6 uL of ...詳細: A 0.6 uL drop of protein solution [15 mg/mL F95M EIZS, 20 mM Tris-HCl (pH 7.5), 300 mM NaCl, 10 mM MgCl2, 10% glycerol, 2 mM TCEP, 2 mM sodium pyrophosphate, 2 mM BTAC] was added to 0.6 uL of precipitant solution [100 mM Bis-Tris (pH 5.5), 200 mM (NH4)2SO4, 25% polyethylene glycol 3350] and equilibrated against a 110 uL well reservoir of precipitant solution. , VAPOR DIFFUSION, SITTING DROP, temperature 298K
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RU200 / 波長: 1.542 Å
検出器
タイプ: RIGAKU RAXIS IV++ / 検出器: IMAGE PLATE / 日付: 2012年8月7日 詳細: Monochromator: Vertically curved graphite crystal.Yields a 40 percent increase in intensitycompared to a flat graphite crystal andequalizes horizontal and vert ical crossfire providing a ...詳細: Monochromator: Vertically curved graphite crystal.Yields a 40 percent increase in intensitycompared to a flat graphite crystal andequalizes horizontal and vert ical crossfire providing a symmetric beam.Mirror: Ni coated, 4-point bend system
放射
モノクロメーター: Vertically curved graphite crystal. Yields a 40 percent increase in intensity compared to a flat graphite crystal and equalizes horizontal and vertical cross fire providing a symmetric beam. プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray