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Yorodumi- PDB-4lqq: Crystal structure of the Cbk1(T743E)-Mob2 kinase-coactivator comp... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4lqq | ||||||
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Title | Crystal structure of the Cbk1(T743E)-Mob2 kinase-coactivator complex in crystal form B | ||||||
Components |
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Keywords | TRANSFERASE/TRANSFERASE activator / Kinase / TRANSFERASE-TRANSFERASE activator complex | ||||||
Function / homology | Function and homology information regulation of fungal-type cell wall organization / budding cell apical bud growth / cellular bud / prospore membrane / establishment or maintenance of actin cytoskeleton polarity / : / incipient cellular bud site / septum digestion after cytokinesis / cellular bud tip / serine/threonine protein kinase complex ...regulation of fungal-type cell wall organization / budding cell apical bud growth / cellular bud / prospore membrane / establishment or maintenance of actin cytoskeleton polarity / : / incipient cellular bud site / septum digestion after cytokinesis / cellular bud tip / serine/threonine protein kinase complex / : / cellular bud neck / mating projection tip / establishment or maintenance of cell polarity / protein kinase activator activity / regulation of protein secretion / cytoplasmic stress granule / cell cortex / non-specific serine/threonine protein kinase / intracellular signal transduction / cell cycle / positive regulation of protein phosphorylation / cell division / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / signal transduction / ATP binding / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.6 Å | ||||||
Authors | Gogl, G. / Remenyi, A. | ||||||
Citation | Journal: Plos Biol. / Year: 2015 Title: The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism. Authors: Gogl, G. / Schneider, K.D. / Yeh, B.J. / Alam, N. / Nguyen Ba, A.N. / Moses, A.M. / Hetenyi, C. / Remenyi, A. / Weiss, E.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4lqq.cif.gz | 217.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4lqq.ent.gz | 163 KB | Display | PDB format |
PDBx/mmJSON format | 4lqq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lq/4lqq ftp://data.pdbj.org/pub/pdb/validation_reports/lq/4lqq | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 58784.582 Da / Num. of mol.: 2 / Fragment: UNP residues 251-756 / Mutation: T743E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: CBK1, N1727, YNL161W / Production host: Escherichia coli (E. coli) References: UniProt: P53894, non-specific serine/threonine protein kinase #2: Protein | Mass: 28415.197 Da / Num. of mol.: 2 / Fragment: UNP residues 46-287 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: MOB2, YFL034C-B, YFL035C, YFL035C-A / Production host: Escherichia coli (E. coli) / References: UniProt: P43563 #3: Chemical | ChemComp-ANP / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 58.75 % |
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Crystal grow | Temperature: 296 K / Method: microbatch under oil / pH: 5.5 Details: four fold excess pepSSD1, 25% PEG 20,000 buffered with 0.1M Na-citrate, pH 5.5, Microbatch under oil, temperature 296K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: May 28, 2011 |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection twin | Operator: h,-k,-l / Fraction: 0.49 |
Reflection | Resolution: 3.6→46.49 Å / Num. all: 23820 / Num. obs: 23529 / % possible obs: 98.8 % / Observed criterion σ(F): 1.36 / Observed criterion σ(I): 1.36 |
Reflection shell | Resolution: 3.6→3.69 Å / % possible all: 91.5 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.6→46.49 Å / σ(F): 1.36 / Phase error: 36.23 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.6→46.49 Å
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Refine LS restraints |
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LS refinement shell |
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