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- PDB-4lqp: Crystal structure of the Cbk1(T743E)-Mob2 kinase-coactivator comp... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4lqp | ||||||
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Title | Crystal structure of the Cbk1(T743E)-Mob2 kinase-coactivator complex, in crystal form A | ||||||
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![]() | TRANSFERASE/TRANSFERASE activator / Kinase / TRANSFERASE-TRANSFERASE activator complex | ||||||
Function / homology | ![]() regulation of fungal-type cell wall organization / budding cell apical bud growth / cellular bud / prospore membrane / establishment or maintenance of actin cytoskeleton polarity / incipient cellular bud site / cellular bud tip / serine/threonine protein kinase complex / septum digestion after cytokinesis / cellular bud neck ...regulation of fungal-type cell wall organization / budding cell apical bud growth / cellular bud / prospore membrane / establishment or maintenance of actin cytoskeleton polarity / incipient cellular bud site / cellular bud tip / serine/threonine protein kinase complex / septum digestion after cytokinesis / cellular bud neck / mating projection tip / actomyosin structure organization / establishment or maintenance of cell polarity / protein kinase activator activity / regulation of protein secretion / mitotic cytokinesis / cytoplasmic stress granule / actin cytoskeleton / cell cortex / non-specific serine/threonine protein kinase / intracellular signal transduction / positive regulation of protein phosphorylation / cell cycle / phosphorylation / cell division / protein serine kinase activity / protein serine/threonine kinase activity / signal transduction / ATP binding / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gogl, G. / Remenyi, A. | ||||||
![]() | ![]() Title: The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism. Authors: Gogl, G. / Schneider, K.D. / Yeh, B.J. / Alam, N. / Nguyen Ba, A.N. / Moses, A.M. / Hetenyi, C. / Remenyi, A. / Weiss, E.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 128.2 KB | Display | ![]() |
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PDB format | ![]() | 93.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 711.1 KB | Display | ![]() |
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Full document | ![]() | 735.2 KB | Display | |
Data in XML | ![]() | 23.8 KB | Display | |
Data in CIF | ![]() | 31.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 58784.582 Da / Num. of mol.: 1 / Fragment: UNP residues 251-756 / Mutation: D475A, T743E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: CBK1, N1727, YNL161W / Production host: ![]() ![]() References: UniProt: P53894, non-specific serine/threonine protein kinase |
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#2: Protein | Mass: 28415.197 Da / Num. of mol.: 1 / Fragment: UNP residues 46-287 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: MOB2, YFL034C-B, YFL035C, YFL035C-A / Production host: ![]() ![]() |
#3: Chemical | ChemComp-ANP / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.56 Å3/Da / Density % sol: 77.88 % |
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Crystal grow | Temperature: 296 K / Method: microbatch under oil / pH: 5.5 Details: 25% PEG 20,000 buffered with 0.1M Na-citrate, pH 5.5, Microbatch under oil, temperature 296K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: May 28, 2011 |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 4.5→49.591 Å / Num. all: 12243 / Num. obs: 12238 / % possible obs: 98.9 % / Observed criterion σ(F): 2.83 / Observed criterion σ(I): 2.83 |
Reflection shell | Resolution: 4.5→4.66 Å / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.5→49.59 Å
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Refine LS restraints |
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LS refinement shell |
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