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Open data
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Basic information
Entry | Database: PDB / ID: 4li0 | ||||||
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Title | Crystal structure of GDP-bound Rab8:GRAB | ||||||
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![]() | PROTEIN TRANSPORT / Small GTPase / Guanine Nucleotide Exchange Factor | ||||||
Function / homology | ![]() guanyl-nucleotide exchange factor activity => GO:0005085 / Golgi to plasma membrane transport vesicle / neurotransmitter receptor transport to postsynaptic membrane / Golgi vesicle fusion to target membrane / vesicle-mediated transport in synapse / regulation of protein transport / VxPx cargo-targeting to cilium / neurotransmitter receptor transport, endosome to postsynaptic membrane / RAB geranylgeranylation / myosin V binding ...guanyl-nucleotide exchange factor activity => GO:0005085 / Golgi to plasma membrane transport vesicle / neurotransmitter receptor transport to postsynaptic membrane / Golgi vesicle fusion to target membrane / vesicle-mediated transport in synapse / regulation of protein transport / VxPx cargo-targeting to cilium / neurotransmitter receptor transport, endosome to postsynaptic membrane / RAB geranylgeranylation / myosin V binding / regulation of exocytosis / trans-Golgi network transport vesicle / RAB GEFs exchange GTP for GDP on RABs / protein localization to cilium / endocytic recycling / non-motile cilium / vesicle docking involved in exocytosis / TBC/RABGAPs / ciliary membrane / ciliary base / exocytosis / Golgi organization / protein secretion / cilium assembly / Anchoring of the basal body to the plasma membrane / centriole / phagocytic vesicle / axonogenesis / protein tyrosine kinase binding / small monomeric GTPase / ciliary basal body / trans-Golgi network membrane / regulation of autophagy / protein localization to plasma membrane / Translocation of SLC2A4 (GLUT4) to the plasma membrane / regulation of long-term neuronal synaptic plasticity / small GTPase binding / autophagy / cilium / recycling endosome membrane / cellular response to insulin stimulus / phagocytic vesicle membrane / GDP binding / Regulation of PLK1 Activity at G2/M Transition / protein transport / synaptic vesicle / midbody / dendritic spine / postsynaptic density / endosome membrane / endosome / Golgi membrane / GTPase activity / centrosome / neuronal cell body / glutamatergic synapse / GTP binding / extracellular exosome / identical protein binding / plasma membrane / cytosol Similarity search - Function | ||||||
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Method | ![]() ![]() ![]() | ||||||
![]() | Guo, Z. / Hou, X.M. / Goody, R.S. / Itzen, A. | ||||||
![]() | ![]() Title: Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Protein Catalyzed by Guanine Nucleotide Exchange Factors Rabin8 and GRAB. Authors: Guo, Z. / Hou, X. / Goody, R.S. / Itzen, A. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 247.3 KB | Display | ![]() |
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PDB format | ![]() | 202.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 24.5 KB | Display | |
Data in CIF | ![]() | 32.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4lhvC ![]() 4lhwC ![]() 4lhxC ![]() 4lhyC ![]() 4lhzC ![]() 1yzqS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Assembly
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Unit cell |
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Components
#1: Protein | Mass: 21259.371 Da / Num. of mol.: 2 / Fragment: unp residues 1-184 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 9800.137 Da / Num. of mol.: 4 / Fragment: unp residues 73-154 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.53 Å3/Da / Density % sol: 72.86 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.3 Details: 1.6 M ammonium sulphate and sodium acetate. cryo solution containing 20% glycerol in the reservoir solution, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 1, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→48.21 Å / Num. all: 19050 / Num. obs: 21124 / % possible obs: 99.6 % / Observed criterion σ(F): 7.6 / Observed criterion σ(I): 9.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1YZQ Resolution: 3.3→48.21 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.896 / SU B: 51.803 / SU ML: 0.391 / Cross valid method: THROUGHOUT / ESU R: 1.408 / ESU R Free: 0.47 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 96.488 Å2
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Refinement step | Cycle: LAST / Resolution: 3.3→48.21 Å
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