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- PDB-4ley: Structure of mouse cGAS bound to 18 bp DNA -

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Basic information

Entry
Database: PDB / ID: 4ley
TitleStructure of mouse cGAS bound to 18 bp DNA
Components
  • 18 bp dsDNA
  • Cyclic GMP-AMP synthase
KeywordsTRANSFERASE/DNA / NTase / DNA sensor / TRANSFERASE-DNA complex
Function / homology
Function and homology information


regulation of type I interferon production / cyclic GMP-AMP synthase / 2',3'-cyclic GMP-AMP synthase activity / paracrine signaling / poly-ADP-D-ribose modification-dependent protein binding / negative regulation of DNA repair / cGAS/STING signaling pathway / regulation of immunoglobulin production / regulation of T cell activation / pattern recognition receptor signaling pathway ...regulation of type I interferon production / cyclic GMP-AMP synthase / 2',3'-cyclic GMP-AMP synthase activity / paracrine signaling / poly-ADP-D-ribose modification-dependent protein binding / negative regulation of DNA repair / cGAS/STING signaling pathway / regulation of immunoglobulin production / regulation of T cell activation / pattern recognition receptor signaling pathway / negative regulation of double-strand break repair via homologous recombination / negative regulation of cGAS/STING signaling pathway / cellular response to exogenous dsRNA / cytoplasmic pattern recognition receptor signaling pathway / cGMP-mediated signaling / cAMP-mediated signaling / nucleosome binding / positive regulation of type I interferon production / regulation of immune response / positive regulation of defense response to virus by host / phosphatidylinositol-4,5-bisphosphate binding / activation of innate immune response / molecular condensate scaffold activity / determination of adult lifespan / positive regulation of cellular senescence / site of double-strand break / double-stranded DNA binding / defense response to virus / nuclear body / DNA repair / innate immune response / DNA damage response / chromatin binding / GTP binding / protein homodimerization activity / DNA binding / nucleoplasm / ATP binding / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Beta Polymerase; domain 2 - #90 / Poly(a)-polymerase, middle domain - #40 / Poly(a)-polymerase, middle domain / Mab-21-like, nucleotidyltransferase domain / Mab-21-like, HhH/H2TH-like domain / Mab-21 protein HhH/H2TH-like domain / Mab-21 protein nucleotidyltransferase domain / Mab-21-like / Mab-21 / Beta Polymerase; domain 2 ...Beta Polymerase; domain 2 - #90 / Poly(a)-polymerase, middle domain - #40 / Poly(a)-polymerase, middle domain / Mab-21-like, nucleotidyltransferase domain / Mab-21-like, HhH/H2TH-like domain / Mab-21 protein HhH/H2TH-like domain / Mab-21 protein nucleotidyltransferase domain / Mab-21-like / Mab-21 / Beta Polymerase; domain 2 / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / Cyclic GMP-AMP synthase
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsLi, P.
CitationJournal: Immunity / Year: 2013
Title: Cyclic GMP-AMP Synthase Is Activated by Double-Stranded DNA-Induced Oligomerization.
Authors: Li, X. / Shu, C. / Yi, G. / Chaton, C.T. / Shelton, C.L. / Diao, J. / Zuo, X. / Kao, C.C. / Herr, A.B. / Li, P.
History
DepositionJun 26, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 25, 2013Provider: repository / Type: Initial release
Revision 1.1Jan 8, 2014Group: Database references
Revision 1.2Feb 28, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_label_asym_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.3Apr 3, 2024Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Cyclic GMP-AMP synthase
B: Cyclic GMP-AMP synthase
C: Cyclic GMP-AMP synthase
D: Cyclic GMP-AMP synthase
E: 18 bp dsDNA
F: 18 bp dsDNA
G: 18 bp dsDNA
H: 18 bp dsDNA
I: 18 bp dsDNA
J: 18 bp dsDNA
K: 18 bp dsDNA
L: 18 bp dsDNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)216,31716
Polymers216,05512
Non-polymers2624
Water11,367631
1
A: Cyclic GMP-AMP synthase
C: Cyclic GMP-AMP synthase
E: 18 bp dsDNA
F: 18 bp dsDNA
I: 18 bp dsDNA
J: 18 bp dsDNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)108,1598
Polymers108,0286
Non-polymers1312
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9760 Å2
ΔGint-38 kcal/mol
Surface area43620 Å2
MethodPISA
2
B: Cyclic GMP-AMP synthase
D: Cyclic GMP-AMP synthase
G: 18 bp dsDNA
H: 18 bp dsDNA
K: 18 bp dsDNA
L: 18 bp dsDNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)108,1598
Polymers108,0286
Non-polymers1312
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9760 Å2
ΔGint-43 kcal/mol
Surface area43320 Å2
MethodPISA
Unit cell
Length a, b, c (Å)78.377, 145.895, 100.963
Angle α, β, γ (deg.)90.00, 90.07, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
Cyclic GMP-AMP synthase / / cGAMP synthase / cGAS / m-cGAS / Mab-21 domain-containing protein 1


Mass: 42984.664 Da / Num. of mol.: 4 / Fragment: Catalytic domain, UNP residues 142-507
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mb21d1 / Production host: Escherichia coli (E. coli)
References: UniProt: Q8C6L5, Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases
#2: DNA chain
18 bp dsDNA


Mass: 5514.603 Da / Num. of mol.: 8 / Source method: obtained synthetically / Details: Synthetic DNA
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 631 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.67 Å3/Da / Density % sol: 53.96 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5
Details: 24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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Data collection

DiffractionMean temperature: 153 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.542 Å
DetectorType: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Mar 31, 2013 / Details: Osmic
RadiationMonochromator: Osmic / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.542 Å / Relative weight: 1
ReflectionResolution: 2.5→40.8 Å / Num. all: 78350 / % possible obs: 99 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.7 % / Rmerge(I) obs: 0.107 / Rsym value: 0.107 / Net I/σ(I): 18.3
Reflection shellResolution: 2.5→2.59 Å / Rmerge(I) obs: 0.716 / Mean I/σ(I) obs: 2 / % possible all: 1

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Processing

Software
NameVersionClassification
CrystalCleardata collection
PHASERphasing
PHENIX(phenix.refine: 1.8.2_1309)refinement
MOSFLMdata reduction
SCALAdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: human cGAS

Resolution: 2.5→40.773 Å / σ(F): 1.34 / Phase error: 28.63 / Stereochemistry target values: TWIN_LSQ_F
RfactorNum. reflection% reflectionSelection details
Rfree0.1911 1993 2.55 %Random
Rwork0.161 ---
obs-78261 99.72 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.5→40.773 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11876 2928 4 631 15439
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00515412
X-RAY DIFFRACTIONf_angle_d0.94221332
X-RAY DIFFRACTIONf_dihedral_angle_d19.7696140
X-RAY DIFFRACTIONf_chiral_restr0.0562316
X-RAY DIFFRACTIONf_plane_restr0.0062208
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.5009-2.56340.32851450.28665411X-RAY DIFFRACTION97
2.5634-2.63270.31741430.26885422X-RAY DIFFRACTION97
2.6327-2.71010.30631460.24825441X-RAY DIFFRACTION97
2.7101-2.79760.27981400.2345434X-RAY DIFFRACTION97
2.7976-2.89750.2451420.2065437X-RAY DIFFRACTION97
2.8975-3.01340.2551440.19525412X-RAY DIFFRACTION97
3.0134-3.15040.22231400.18055436X-RAY DIFFRACTION97
3.1504-3.31630.21551400.15845403X-RAY DIFFRACTION97
3.3163-3.52380.19081410.15235471X-RAY DIFFRACTION97
3.5238-3.79540.17891430.15175414X-RAY DIFFRACTION97
3.7954-4.17650.17061410.13385438X-RAY DIFFRACTION97
4.1765-4.77890.15031460.11425452X-RAY DIFFRACTION97
4.7789-6.01330.12911420.12115488X-RAY DIFFRACTION97
6.0133-30.51650.12861400.12635507X-RAY DIFFRACTION97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.05030.00150.03010.0403-0.05120.0905-0.006-0.2718-0.04840.05290.1566-0.0227-0.0310.04940.00280.2106-0.02510.02050.2328-0.02490.256137.0976-1.796437.5737
20.0477-0.05150.01520.0433-0.01620.03750.03320.0815-0.0564-0.05190.0564-0.09280.0118-0.04060.02580.26410.09120.07860.14570.08260.345430.8988-8.431630.1544
30.01940.03590.06220.05990.09420.16450.00210.08960.0620.00190.0743-0.11180.2349-0.16970.00020.1568-0.0496-0.06140.40280.0960.279515.9135-9.357720.0079
40.0020.00110.00370.00330.00390.0049-0.090.074-0.0455-0.0876-0.00170.04250.099-0.021500.3846-0.1398-0.09040.3710.00860.449213.1831-23.111318.5202
50.01540.0159-0.03540.0663-0.02310.0685-0.0076-0.1579-0.15110.01120.10850.13530.0023-0.1259-00.19550.00680.00540.27250.0940.274824.3413-13.07938.0082
60.0560.06750.00880.1519-0.12920.2770.11680.08920.0613-0.06890.1030.1460.1113-0.24560.45760.13510.0312-0.02360.3380.18260.149623.88581.270212.1767
70.313-0.1269-0.14230.34650.31640.29170.01510.0310.1220.170.1679-0.0037-0.0344-0.09540.23580.16570.023-0.04030.35020.16930.271416.9031-4.213324.6877
80.1241-0.026-0.27320.00080.07710.55330.27780.10360.32860.08380.07880.0918-0.6147-0.40770.99880.3240.32680.27320.11330.07710.326723.75314.247227.308
90.0455-0.00540.08740.0018-0.01270.17360.10180.02040.13570.00970.1777-0.0956-0.18740.03270.1960.62180.27090.30670.45880.00510.560917.731820.519740.2512
100.06620.02570.05910.0201-0.00050.09570.0005-0.28130.0263-0.0370.05760.13210.0083-0.07470.02280.1471-0.0032-0.0310.2127-0.04170.24481.94844.002368.0974
110.0438-0.0010.04850.01250.00210.04860.09040.07220.04360.0567-0.03160.0794-0.0581-0.125700.24760.033-0.01960.2114-0.02340.21219.000651.054460.5329
120.08570.06170.13140.3611-0.00110.2179-0.0601-0.0110.0693-0.0139-0.0885-0.0335-0.14080.0259-0.13540.1787-0.0013-0.05190.2527-0.03860.136220.035356.668660.4036
130.21090.00340.01550.15570.05110.16840.06110.0322-0.2959-0.023-0.0116-0.17250.09670.0965-0.00770.12440.0195-0.06560.1852-0.00270.231316.753132.22755.8005
140.0473-0.0023-0.09430.00960.01610.1638-0.03020.1012-0.0267-0.0550.1127-0.0489-0.0584-0.17990.0020.1934-0.0576-0.0130.21240.02370.193441.1385-1.8799-13.4853
150.1955-0.0035-0.16560.16820.03530.1324-0.02720.0387-0.07180.04220.0616-0.01890.1560.1112-00.1778-0.0087-0.0210.2121-0.02640.169354.857-10.2922-6.3794
160.23270.02680.02550.1642-0.10530.30750.08480.03670.07520.0430.1050.0953-0.14490.17610.01750.2174-0.04270.04080.2068-0.00550.18854.650411.7342-4.4487
170.25490.05720.19890.1069-0.05750.2303-0.01220.21450.09420.05120.0426-0.00280.02990.01970.00530.2085-0.0176-0.03280.2702-0.04910.2166-8.184149.471521.6915
180.0489-0.00260.01820.0005-0.00080.0064-0.0123-0.03640.03450.0750.08990.0081-0.102-0.05580.0460.39890.0956-0.16510.21230.01410.611-21.140968.319331.6832
190.0259-0.06550.03610.2148-0.11820.0607-0.06950.12640.05250.11380.0416-0.0704-0.0121-0.0684-0.00560.1273-0.0344-0.020.2596-0.04320.1658-13.822350.553124.212
200.09140.1294-0.05750.2914-0.26550.64870.06250.071-0.0956-0.01470.1120.04370.2298-0.21750.31270.2433-0.0624-0.08170.2386-0.08920.2499-15.70428.320423.1336
210.0006-0.00010.00240.00160.00170.0062-0.07880.0485-0.0325-0.0699-0.0251-0.10180.0034-0.0621-0.00030.84240.0145-0.18120.442-0.12990.711747.3058-30.755413.4841
220.04830.0861-0.05050.1636-0.09120.11860.1325-0.0583-0.07520.02460.00250.18920.02480.09810.03750.2286-0.0679-0.09220.1465-0.03330.179347.8941-7.41724.2885
230.00410.00180.00040.00110.00210.00770.00740.0019-0.04740.09820.0112-0.0042-0.0386-0.0368-0.00010.3548-0.12630.03740.3033-0.04430.292848.59699.770737.637
240.00220.00280.00150.00180.00130.0007-0.00060.0382-0.0265-0.0721-0.0428-0.0183-0.0510.09320.00020.3164-0.18070.05630.43-0.11130.503454.03297.005432.2329
250.0150.0015-0.00580.0057-0.00860.01050.1441-0.0512-0.1033-0.07090.0889-0.11430.1052-0.01770.00020.21420.0293-0.0130.2185-0.07090.207343.1806-5.485124.718
260.0081-0.0045-0.00660.00620.0040.00630.06530.0328-0.0535-0.0160.00960.02870.00420.001300.550.1037-0.07560.2416-0.06910.45151.3004-21.160519.0825
270.00710.001-0.00230-00.00060.00410.0165-0.0055-0.0002-0.0096-0.0135-0.0064-0.0627-00.738-0.2407-0.00080.4614-0.10490.915144.501-35.083219.2814
280.00180.00090.00080.00110.00280.00610.0508-0.1046-0.0028-0.0712-0.0171-0.0812-0.0271-0.0114-00.438-0.1232-0.05880.2621-0.00020.4368-6.574362.136754.6155
29-0.0001-0.0007-0.0002-0.00020.0004-0.0006-0.1127-0.05570.02960.0076-0.19150.0913-0.0199-0.0229-00.32770.0210.0320.223-0.02510.2301-10.505944.27853.2588
300.01980.00760.00050.02340.00210.00380.0837-0.0836-0.0127-0.05550.08850.01670.08480.01420.00430.3681-0.14550.10970.34310.0350.5612-10.096631.845467.1972
310.00230.00360.00080.00570.00220.00090.04640.01130.015-0.0084-0.04050.03140.0418-0.0147-0.00010.6968-0.26130.00480.6793-0.05960.9187-19.861720.232366.5064
320.0454-0.0164-0.0380.01170.01210.0333-0.02220.0459-0.0556-0.0509-0.075-0.0273-0.0038-0.0258-0.00391.0293-0.15020.1890.3418-0.07481.1954-12.568421.199363.5368
330.0858-0.0293-0.01150.0368-0.02830.05190.1006-0.2005-0.1143-0.2204-0.19090.2703-0.16620.0753-0.00130.35710.0003-0.01430.2624-0.02240.3156-9.640949.502355.7721
340.00110.0016-0.00170.0021-0.00190.00120.061-0.00380.03410.01390.01350.03660.0010.0088-00.8552-0.3143-0.1360.90320.03440.819829.1524-17.4192-3.9536
350.04340.0026-0.02470.00520.01580.08520.2165-0.10440.0764-0.1735-0.00630.19680.0454-0.14890.01150.25580.0568-0.02720.39170.16380.510225.264912.6449-8.6002
360.0005-0.0009-0.00130.00090.00060.0037-0.0788-0.03470.01530.0021-0.11250.017-0.01020.01980.00010.9049-0.00750.07220.51530.11811.127426.754925.3426-8.9974
370.02560.0263-0.00890.01950.0030.04690.14180.14080.20490.1007-0.18380.16340.2616-0.0337-0.00020.4318-0.0525-0.00170.34160.10120.355626.6703-3.8195-4.164
380.004-0.004-0.00410.0050.00210.0061-0.0367-0.04320.0063-0.0126-0.07880.00840.0409-0.040400.70190.01520.05060.3198-0.02030.585710.830877.173433.7344
390.3188-0.19720.11150.144-0.08520.12240.11670.24340.122-0.105-0.1595-0.15880.02260.0357-0.15150.2960.0450.06120.2785-0.06850.251711.620247.688425.651
400.0147-0.01210.02790.009-0.02070.0409-0.06460.1703-0.11850.13680.0391-0.1877-0.14810.0147-00.353-0.0055-0.00940.4522-0.16410.37511.071243.723525.0493
410.01240.00560.00720.00270.00240.00660.11730.067-0.03480.0173-0.10640.0108-0.05560.026800.37170.058-0.02030.2718-0.02660.337315.594567.377430.1669
420.0079-0.00230.00110.00630.00260.00890.04080.04650.0182-0.0043-0.01530.01950.0131-0.02800.53920.2134-0.00080.6361-0.04080.68727.528980.087927.142
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 149 through 183 )
2X-RAY DIFFRACTION2chain 'A' and (resid 184 through 210 )
3X-RAY DIFFRACTION3chain 'A' and (resid 211 through 239 )
4X-RAY DIFFRACTION4chain 'A' and (resid 240 through 258 )
5X-RAY DIFFRACTION5chain 'A' and (resid 259 through 314 )
6X-RAY DIFFRACTION6chain 'A' and (resid 315 through 349 )
7X-RAY DIFFRACTION7chain 'A' and (resid 350 through 376 )
8X-RAY DIFFRACTION8chain 'A' and (resid 377 through 482 )
9X-RAY DIFFRACTION9chain 'A' and (resid 483 through 507 )
10X-RAY DIFFRACTION10chain 'B' and (resid 149 through 183 )
11X-RAY DIFFRACTION11chain 'B' and (resid 184 through 219 )
12X-RAY DIFFRACTION12chain 'B' and (resid 220 through 314 )
13X-RAY DIFFRACTION13chain 'B' and (resid 315 through 507 )
14X-RAY DIFFRACTION14chain 'C' and (resid 149 through 210 )
15X-RAY DIFFRACTION15chain 'C' and (resid 211 through 333 )
16X-RAY DIFFRACTION16chain 'C' and (resid 334 through 507 )
17X-RAY DIFFRACTION17chain 'D' and (resid 149 through 239 )
18X-RAY DIFFRACTION18chain 'D' and (resid 240 through 258 )
19X-RAY DIFFRACTION19chain 'D' and (resid 259 through 376 )
20X-RAY DIFFRACTION20chain 'D' and (resid 377 through 507 )
21X-RAY DIFFRACTION21chain 'E' and (resid 1 through 5 )
22X-RAY DIFFRACTION22chain 'E' and (resid 6 through 15 )
23X-RAY DIFFRACTION23chain 'E' and (resid 16 through 18 )
24X-RAY DIFFRACTION24chain 'F' and (resid 1 through 5 )
25X-RAY DIFFRACTION25chain 'F' and (resid 6 through 10 )
26X-RAY DIFFRACTION26chain 'F' and (resid 11 through 15 )
27X-RAY DIFFRACTION27chain 'F' and (resid 16 through 18 )
28X-RAY DIFFRACTION28chain 'G' and (resid 1 through 5 )
29X-RAY DIFFRACTION29chain 'G' and (resid 6 through 10 )
30X-RAY DIFFRACTION30chain 'G' and (resid 11 through 15 )
31X-RAY DIFFRACTION31chain 'G' and (resid 16 through 18 )
32X-RAY DIFFRACTION32chain 'H' and (resid 1 through 5 )
33X-RAY DIFFRACTION33chain 'H' and (resid 6 through 18 )
34X-RAY DIFFRACTION34chain 'I' and (resid 1 through 5 )
35X-RAY DIFFRACTION35chain 'I' and (resid 6 through 18 )
36X-RAY DIFFRACTION36chain 'J' and (resid 1 through 5 )
37X-RAY DIFFRACTION37chain 'J' and (resid 6 through 18 )
38X-RAY DIFFRACTION38chain 'K' and (resid 1 through 5 )
39X-RAY DIFFRACTION39chain 'K' and (resid 6 through 18 )
40X-RAY DIFFRACTION40chain 'L' and (resid 1 through 10 )
41X-RAY DIFFRACTION41chain 'L' and (resid 11 through 15 )
42X-RAY DIFFRACTION42chain 'L' and (resid 16 through 18 )

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