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- PDB-4lea: The Crystal Structure of Pyocin L1 bound to D-mannose at 2.55 Ang... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4lea | ||||||
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Title | The Crystal Structure of Pyocin L1 bound to D-mannose at 2.55 Angstroms | ||||||
![]() | Pyocin L1 | ||||||
![]() | SUGAR BINDING PROTEIN / Mannose Monocot Binding Protein / MMBL / Galanthus nivalis Agglutinin / Beta Prism / Protein Antibiotic / Extra cellular | ||||||
Function / homology | ![]() ![]() ![]() Similarity search - Domain/homology | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Grinter, R. / Roszak, A.W. / Mccaughey, L. / Cogdell, C.J. / Walker, D. | ||||||
![]() | ![]() Title: Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor. Authors: McCaughey, L.C. / Grinter, R. / Josts, I. / Roszak, A.W. / Walen, K.I. / Cogdell, R.J. / Milner, J. / Evans, T. / Kelly, S. / Tucker, N.P. / Byron, O. / Smith, B. / Walker, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 113.3 KB | Display | ![]() |
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PDB format | ![]() | 88.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4le7SC ![]() 4ledC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30008.020 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Sugar | ChemComp-BMA / ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.27 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 20% PEG 550 MME, 20% PEG 20K, 0.03 M CaCl2, 0.03 M MgCl2 0.1 M MOPS/HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Dec 14, 2012 |
Radiation | Monochromator: Silicon / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.55→55.53 Å / Num. obs: 22096 / % possible obs: 99.9 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 5.5 % / Biso Wilson estimate: 61.517 Å2 / Rmerge(I) obs: 0.059 / Net I/σ(I): 13.3 |
Reflection shell | Resolution: 2.55→2.67 Å / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 4LE7 Resolution: 2.55→42.28 Å / σ(F): 2 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Refinement step | Cycle: LAST / Resolution: 2.55→42.28 Å
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