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- PDB-4l1i: Crystal structure of EGFP-based Calcium Sensor CatchER complexed ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4l1i | ||||||
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Title | Crystal structure of EGFP-based Calcium Sensor CatchER complexed with Ca | ||||||
![]() | EGFP-based Calcium Sensor CatchER | ||||||
![]() | CALCIUM BINDING PROTEIN / chromophore / luminescence / fluorescent protein / calcium sensor / metal ions / gadolinium | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, Y. / Weber, I.T. | ||||||
![]() | ![]() Title: Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics. Authors: Zhang, Y. / Reddish, F. / Tang, S. / Zhuo, Y. / Wang, Y.F. / Yang, J.J. / Weber, I.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 120 KB | Display | ![]() |
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PDB format | ![]() | 91.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433 KB | Display | ![]() |
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Full document | ![]() | 436.8 KB | Display | |
Data in XML | ![]() | 13.5 KB | Display | |
Data in CIF | ![]() | 19.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4l12C ![]() 4l13C ![]() 2okwS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26141.316 Da / Num. of mol.: 1 / Fragment: SEE REMARK 999 Mutation: F64L, S65T, S147E, M153T, V163A, S202D, Q204E, F223E, T225E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | RESIDUE SER 65 UNDERWENT MUTATION TO THR 65. THR 65, TYR 66, AND GLY 67 CIRCULARIZ | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.37 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.9 mM protein, 53 mM HEPES, pH 7.0, 1 mM beta-mercaptoethanol, 50 mM sodium acetate, 16% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Nov 17, 2010 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 1.2→50 Å / Num. all: 70349 / Num. obs: 70349 / % possible obs: 99.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 12.5 % / Rmerge(I) obs: 0.081 / Net I/σ(I): 19.9 |
Reflection shell | Resolution: 1.2→1.24 Å / Redundancy: 7.8 % / Rmerge(I) obs: 0.392 / Mean I/σ(I) obs: 8.7 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2OKW Resolution: 1.2→50 Å / Num. parameters: 19518 / Num. restraintsaints: 25624 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER Details: ANISOTROPIC REFINEMENT REDUCED FREE R (NO CUTOFF) BY ?
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Refine analyze | Num. disordered residues: 23 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 2019.61 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.2→50 Å
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Refine LS restraints |
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