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- PDB-4kod: Structure of p97 N-D1 R155H mutant in complex with ADP -

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Basic information

Entry
Database: PDB / ID: 4kod
TitleStructure of p97 N-D1 R155H mutant in complex with ADP
ComponentsTransitional endoplasmic reticulum ATPase
KeywordsHYDROLASE / AAA ATPase / Transport protein
Function / homology
Function and homology information


positive regulation of Lys63-specific deubiquitinase activity / flavin adenine dinucleotide catabolic process / positive regulation of protein K63-linked deubiquitination / protein-DNA covalent cross-linking repair / positive regulation of oxidative phosphorylation / ATPase complex / VCP-NSFL1C complex / deubiquitinase activator activity / endosome to lysosome transport via multivesicular body sorting pathway / cellular response to arsenite ion ...positive regulation of Lys63-specific deubiquitinase activity / flavin adenine dinucleotide catabolic process / positive regulation of protein K63-linked deubiquitination / protein-DNA covalent cross-linking repair / positive regulation of oxidative phosphorylation / ATPase complex / VCP-NSFL1C complex / deubiquitinase activator activity / endosome to lysosome transport via multivesicular body sorting pathway / cellular response to arsenite ion / endoplasmic reticulum stress-induced pre-emptive quality control / Derlin-1 retrotranslocation complex / BAT3 complex binding / ERAD pathway / mitotic spindle disassembly / VCP-NPL4-UFD1 AAA ATPase complex / NADH metabolic process / aggresome assembly / vesicle-fusing ATPase / regulation of protein localization to chromatin / ER-associated misfolded protein catabolic process / K48-linked polyubiquitin modification-dependent protein binding / stress granule disassembly / positive regulation of mitochondrial membrane potential / retrograde protein transport, ER to cytosol / autophagosome maturation / regulation of aerobic respiration / MHC class I protein binding / regulation of synapse organization / positive regulation of ATP biosynthetic process / negative regulation of smoothened signaling pathway / ubiquitin-specific protease binding / ubiquitin-like protein ligase binding / ATP metabolic process / RHOH GTPase cycle / Attachment and Entry / polyubiquitin modification-dependent protein binding / proteasomal protein catabolic process / endoplasmic reticulum to Golgi vesicle-mediated transport / Protein methylation / translesion synthesis / HSF1 activation / proteasome complex / interstrand cross-link repair / endoplasmic reticulum unfolded protein response / lipid droplet / ubiquitin-dependent ERAD pathway / Josephin domain DUBs / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / macroautophagy / ADP binding / Hh mutants are degraded by ERAD / Defective CFTR causes cystic fibrosis / Hedgehog ligand biogenesis / Translesion Synthesis by POLH / ABC-family proteins mediated transport / positive regulation of protein catabolic process / positive regulation of protein-containing complex assembly / double-strand break repair / establishment of protein localization / Aggrephagy / cytoplasmic stress granule / autophagy / positive regulation of canonical Wnt signaling pathway / azurophil granule lumen / activation of cysteine-type endopeptidase activity involved in apoptotic process / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / site of double-strand break / viral genome replication / Ovarian tumor domain proteases / Attachment and Entry / E3 ubiquitin ligases ubiquitinate target proteins / proteasome-mediated ubiquitin-dependent protein catabolic process / cellular response to heat / secretory granule lumen / protein phosphatase binding / regulation of apoptotic process / protein ubiquitination / ficolin-1-rich granule lumen / lipid binding / : / glutamatergic synapse / DNA repair / protein domain specific binding / intracellular membrane-bounded organelle / cellular response to DNA damage stimulus / ubiquitin protein ligase binding / Neutrophil degranulation / endoplasmic reticulum membrane / perinuclear region of cytoplasm / endoplasmic reticulum / protein-containing complex / RNA binding / extracellular exosome / extracellular region / nucleoplasm / ATP binding / identical protein binding / nucleus / cytosol
Similarity search - Function
Vcp-like ATPase; Chain A, domain 2 - #10 / Vcp-like ATPase; Chain A, domain 2 / AAA ATPase, CDC48 family / Barwin-like endoglucanases - #20 / Cell division protein 48 (CDC48), N-terminal domain / CDC48, N-terminal subdomain / Cell division protein 48 (CDC48) N-terminal domain / CDC48, domain 2 / Cell division protein 48 (CDC48), domain 2 / Cell division protein 48 (CDC48) domain 2 ...Vcp-like ATPase; Chain A, domain 2 - #10 / Vcp-like ATPase; Chain A, domain 2 / AAA ATPase, CDC48 family / Barwin-like endoglucanases - #20 / Cell division protein 48 (CDC48), N-terminal domain / CDC48, N-terminal subdomain / Cell division protein 48 (CDC48) N-terminal domain / CDC48, domain 2 / Cell division protein 48 (CDC48), domain 2 / Cell division protein 48 (CDC48) domain 2 / CDC48 domain 2-like superfamily / Helicase, Ruva Protein; domain 3 - #60 / Vps4 C terminal oligomerisation domain / Vps4 oligomerisation, C-terminal / Barwin-like endoglucanases / Aspartate decarboxylase-like domain superfamily / AAA+ lid domain / AAA ATPase, AAA+ lid domain / AAA-protein family signature. / ATPase, AAA-type, conserved site / Helicase, Ruva Protein; domain 3 / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleotide triphosphate hydrolases / Roll / Beta Barrel / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
ADENOSINE-5'-DIPHOSPHATE / Transitional endoplasmic reticulum ATPase
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.96 Å
AuthorsXia, D. / Tang, W.K.
CitationJournal: J.Biol.Chem. / Year: 2013
Title: Altered Intersubunit Communication Is the Molecular Basis for Functional Defects of Pathogenic p97 Mutants.
Authors: Tang, W.K. / Xia, D.
History
DepositionMay 11, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 13, 2013Provider: repository / Type: Initial release
Revision 1.1Jan 8, 2014Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Transitional endoplasmic reticulum ATPase
B: Transitional endoplasmic reticulum ATPase
C: Transitional endoplasmic reticulum ATPase
D: Transitional endoplasmic reticulum ATPase
E: Transitional endoplasmic reticulum ATPase
F: Transitional endoplasmic reticulum ATPase
G: Transitional endoplasmic reticulum ATPase
H: Transitional endoplasmic reticulum ATPase
I: Transitional endoplasmic reticulum ATPase
J: Transitional endoplasmic reticulum ATPase
K: Transitional endoplasmic reticulum ATPase
L: Transitional endoplasmic reticulum ATPase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)659,87424
Polymers654,74712
Non-polymers5,12612
Water3,027168
1
A: Transitional endoplasmic reticulum ATPase
B: Transitional endoplasmic reticulum ATPase
C: Transitional endoplasmic reticulum ATPase
D: Transitional endoplasmic reticulum ATPase
E: Transitional endoplasmic reticulum ATPase
F: Transitional endoplasmic reticulum ATPase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)329,93712
Polymers327,3746
Non-polymers2,5636
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area18720 Å2
ΔGint-74 kcal/mol
Surface area108570 Å2
MethodPISA
2
G: Transitional endoplasmic reticulum ATPase
H: Transitional endoplasmic reticulum ATPase
I: Transitional endoplasmic reticulum ATPase
J: Transitional endoplasmic reticulum ATPase
K: Transitional endoplasmic reticulum ATPase
L: Transitional endoplasmic reticulum ATPase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)329,93712
Polymers327,3746
Non-polymers2,5636
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area18370 Å2
ΔGint-73 kcal/mol
Surface area111510 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)146.520, 170.737, 256.601
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21B
31C
41D
51E
61F
71G
81H
91I
101J
111L
12A
22C
32D
42E
52G
62I
72J
82K
13A
23K
14A
24L
15A
25F
16A
26H
17A
27B

NCS domain segments:
Dom-IDComponent-IDEns-IDRefine codeAuth asym-IDAuth seq-ID
1111A201 - 431
2111B201 - 431
3111C201 - 431
4111D201 - 431
5111E201 - 431
6111F201 - 431
7111G201 - 431
8111H201 - 431
9111I201 - 431
10111J201 - 431
11111L201 - 431
1211A435 - 459
2211B435 - 459
3211C435 - 459
4211D435 - 459
5211E435 - 459
6211F435 - 459
7211G435 - 459
8211H435 - 459
9211I435 - 459
10211J435 - 459
11211L435 - 459
1311A800
2311B800
3311C800
4311D800
5311E800
6311F800
7311G800
8311H800
9311I800
10311J800
11311L800
1121A24 - 107
2121C24 - 107
3121D24 - 107
4121E24 - 107
5121G24 - 107
6121I24 - 107
7121J24 - 107
8121K24 - 107
1221A108 - 195
2221C108 - 195
3221D108 - 195
4221E108 - 195
5221G108 - 195
6221I108 - 195
7221J108 - 195
8221K108 - 195
1131A368 - 431
2131K368 - 431
1231A435 - 459
2231K435 - 459
1331A800
2331K800
1431A200 - 236
2431K200 - 236
1531A246 - 264
2531K246 - 264
1141A24 - 107
2141L24 - 107
1241A108 - 195
2241L108 - 195
1151A24 - 51
2151F24 - 51
1251A53 - 107
2251F53 - 107
1351A116 - 195
2351F116 - 195
1161A24 - 51
2161H24 - 51
1261A53 - 107
2261H53 - 107
1361A108 - 195
2361H108 - 195
1171A24 - 107
2171B24 - 107
1271A108 - 195
2271B108 - 195

NCS ensembles :
ID
1
2
3
4
5
6
7

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Components

#1: Protein
Transitional endoplasmic reticulum ATPase / TER ATPase / 15S Mg(2+)-ATPase p97 subunit / Valosin-containing protein / VCP


Mass: 54562.266 Da / Num. of mol.: 12 / Fragment: UNP residue 1-481 / Mutation: R155HH
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: p97, VCP / Production host: Escherichia coli (E. coli) / References: UniProt: P55072, vesicle-fusing ATPase
#2: Chemical
ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / Adenosine diphosphate


Mass: 427.201 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Comment: ADP, energy-carrying molecule*YM
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 168 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.45 Å3/Da / Density % sol: 49.82 %
Crystal growTemperature: 288 K / Method: vapor diffusion, sitting drop / pH: 5.8
Details: 0.1M citrate, pH 5.8, 0.3M NaCl, 13.6% EPG 3350, 20% glycerol, 0.625% benzamidine, VAPOR DIFFUSION, SITTING DROP, temperature 288K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jan 1, 2008
RadiationMonochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.69→21.99 Å / Num. obs: 118808 / % possible obs: 88.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3
Reflection shellResolution: 2.95→3.06 Å / % possible all: 51.3

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Processing

Software
NameVersionClassification
HKL-2000data collection
MOLREPphasing
REFMAC5.5.0102refinement
DENZOdata reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.96→21.99 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.898 / SU B: 71.101 / SU ML: 0.534 / Cross valid method: THROUGHOUT / ESU R Free: 0.558 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.29094 5937 5 %RANDOM
Rwork0.26841 ---
obs0.26956 112289 88.01 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 120.557 Å2
Baniso -1Baniso -2Baniso -3
1-3.67 Å20 Å20 Å2
2---9.12 Å20 Å2
3---5.45 Å2
Refinement stepCycle: LAST / Resolution: 2.96→21.99 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms41268 0 324 168 41760
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0080.01942265
X-RAY DIFFRACTIONr_bond_other_d0.0020.0241544
X-RAY DIFFRACTIONr_angle_refined_deg1.2561.99357179
X-RAY DIFFRACTIONr_angle_other_deg0.807395722
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.18255258
X-RAY DIFFRACTIONr_dihedral_angle_2_deg35.59524.2441963
X-RAY DIFFRACTIONr_dihedral_angle_3_deg18.914157724
X-RAY DIFFRACTIONr_dihedral_angle_4_deg16.42215379
X-RAY DIFFRACTIONr_chiral_restr0.0640.26519
X-RAY DIFFRACTIONr_gen_planes_refined0.0050.02147290
X-RAY DIFFRACTIONr_gen_planes_other0.0020.028949
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.5251.526285
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it0.978242563
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it0.948315975
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it1.7684.514611
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION

Ens-IDDom-IDAuth asym-IDNumberTypeRms dev position (Å)Weight position
11A2001TIGHT POSITIONAL0.050.05
12B2001TIGHT POSITIONAL0.050.05
13C2001TIGHT POSITIONAL0.040.05
14D2001TIGHT POSITIONAL0.050.05
15E2001TIGHT POSITIONAL0.050.05
16F2001TIGHT POSITIONAL0.050.05
17G2001TIGHT POSITIONAL0.050.05
18H2001TIGHT POSITIONAL0.050.05
19I2001TIGHT POSITIONAL0.040.05
110J2001TIGHT POSITIONAL0.050.05
111L2001TIGHT POSITIONAL0.040.05
11A2001TIGHT THERMAL0.070.5
12B2001TIGHT THERMAL0.080.5
13C2001TIGHT THERMAL0.070.5
14D2001TIGHT THERMAL0.070.5
15E2001TIGHT THERMAL0.070.5
16F2001TIGHT THERMAL0.070.5
17G2001TIGHT THERMAL0.070.5
18H2001TIGHT THERMAL0.080.5
19I2001TIGHT THERMAL0.070.5
110J2001TIGHT THERMAL0.090.5
111L2001TIGHT THERMAL0.060.5
21A1373TIGHT POSITIONAL0.050.05
22C1373TIGHT POSITIONAL0.040.05
23D1373TIGHT POSITIONAL0.040.05
24E1373TIGHT POSITIONAL0.030.05
25G1373TIGHT POSITIONAL0.040.05
26I1373TIGHT POSITIONAL0.040.05
27J1373TIGHT POSITIONAL0.050.05
28K1373TIGHT POSITIONAL0.040.05
21A1373TIGHT THERMAL0.720.5
22C1373TIGHT THERMAL0.120.5
23D1373TIGHT THERMAL0.110.5
24E1373TIGHT THERMAL0.10.5
25G1373TIGHT THERMAL0.120.5
26I1373TIGHT THERMAL0.110.5
27J1373TIGHT THERMAL0.130.5
28K1373TIGHT THERMAL0.110.5
31A1119TIGHT POSITIONAL0.070.05
31A1119TIGHT THERMAL0.110.5
41A1373TIGHT POSITIONAL0.040.05
41A1373TIGHT THERMAL0.140.5
51A1292TIGHT POSITIONAL0.070.05
51A1292TIGHT THERMAL0.320.5
61A1362TIGHT POSITIONAL0.050.05
61A1362TIGHT THERMAL0.120.5
71A1373TIGHT POSITIONAL0.060.05
71A1373TIGHT THERMAL0.110.5
LS refinement shellResolution: 2.96→3.035 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.525 244 -
Rwork0.521 4575 -
obs--49.93 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
17.69082.77680.22448.0593-0.21138.2499-0.54841.10380.1585-0.50920.4168-0.49010.25070.8840.13150.2437-0.21030.04611.02240.1530.4618103.869232.79642.4663
211.11851.6375-4.72024.73641.08988.1398-0.2887-0.59830.84110.18430.4117-0.245-0.46670.2665-0.12310.4139-0.167-0.08910.94420.12210.7701104.727643.958259.9426
38.6353-0.97591.5151.0860.19185.3904-0.22730.60190.30170.0994-0.0722-0.1509-0.1247-0.34180.29950.3364-0.1544-0.06480.83640.1430.102470.508630.029747.0979
47.7591.48190.4726.26424.14889.02640.1859-0.5895-0.00860.6129-0.0573-0.40791.11120.5656-0.12860.46480.0626-0.20611.00860.2430.272991.919124.089373.2841
527.518311.492728.181131.785216.993629.87340.75970.76690.06370.6526-0.712-0.39350.85360.5574-0.04770.9401-0.097-0.03690.56310.24630.531881.272225.465460.2869
69.44970.54732.32525.8961.23048.6047-0.16130.2358-0.52580.3793-0.0420.6930.6601-0.54060.20340.2724-0.13750.06580.91340.16770.2246-2.730517.978716.498
75.6532-0.26461.90946.14860.0157.31620.24990.1986-0.1416-0.20950.0109-0.2317-0.1664-0.0939-0.26080.3017-0.0042-0.04820.96870.17060.105510.315429.7284.9136
83.8206-0.37851.34871.54120.14848.29130.0579-0.52210.4788-0.03610.0535-0.028-0.1390.1839-0.11140.4083-0.02040.07560.86090.15760.171117.531622.473842.7203
95.74730.9499-1.45597.0709-2.0246.6801-0.12090.8732-0.8108-0.2431-0.0123-0.42990.4680.54290.13320.42230.0821-0.0070.8913-0.06890.174431.074114.62112.1479
1023.92966.577323.018916.40559.721241.4933-0.35730.26950.60120.2558-0.73850.71270.53350.04331.09590.7999-0.01440.02670.538-0.0120.751424.658516.976127.5343
1111.63790.1016-2.6726.93361.106310.5161-0.0138-1.6011-0.55440.6062-0.2191-0.48440.25660.23080.23290.70430.02370.16821.15090.19140.263827.4921.6098122.1901
1210.55490.61091.70586.2853-3.49729.4520.0275-0.93340.90140.12870.2150.6889-0.5331-0.968-0.24250.72560.14410.24941.1764-0.09810.30278.738326.3039114.605
136.80610.69870.94522.2310.06565.843-0.61810.85440.115-0.03460.12410.0864-0.34280.9990.4940.7399-0.13630.16721.2414-0.0220.124740.17126.096291.3506
1411.5923-2.23344.35554.6796-1.09035.7813-0.05440.1668-0.8066-0.26250.1277-0.090.4746-0.0717-0.07330.6554-0.20090.27380.514-0.07620.295711.47937.802395.2251
156.57611.4048-10.104316.8053-2.932815.65580.3124-0.2794-0.41891.2172-0.8207-0.2572-0.77780.52190.50830.8749-0.04330.06670.8150.01760.871826.230815.434493.217
168.55980.0672-0.1738.058-0.45877.2212-0.0115-0.1687-0.07570.72730.50540.5557-0.5956-1.2458-0.49390.35890.10150.29631.09950.15940.3944-17.225511.380778.1048
178.2310.6102-0.73554.3328-1.96610.62540.07760.13820.64070.11560.1870.6271-0.7369-1.0189-0.26460.34360.340.21891.01830.260.5614-21.727222.226160.7999
186.03622.9240.93032.6992-0.10254.24850.3343-0.5552-0.0970.5149-0.21090.132-0.67630.7773-0.12340.6368-0.17580.11591.082-0.02790.092915.29320.112373.4235
199.1088-2.58373.69624.0705-3.31679.4166-0.03210.74640.165-0.32640.22230.04050.8352-0.5547-0.19030.4394-0.18070.08350.83580.12210.1383-2.88368.283846.9334
202.16775.82763.231815.69998.7064.860.4584-0.1375-0.12861.0834-0.3402-0.37920.5333-0.247-0.11820.9501-0.03830.02720.6490.07330.8036.577912.684460.0142
2111.2158-0.0564-0.75747.43810.64635.54430.0982-1.19940.38780.5942-0.2153-0.87070.07821.39470.11710.7742-0.193-0.36221.25950.09030.366388.138333.1049104.618
2210.6698-1.0042-4.24473.8564-1.41065.29470.6874-1.54261.2811.1653-0.37040.3591-1.19980.4156-0.3171.5508-0.3609-0.13831.6075-0.53440.650172.333740.7972115.6675
233.6369-0.17111.51322.0835-0.30116.5261-0.13970.6550.24680.11940.0124-0.0666-0.09340.15990.12730.334-0.1072-0.02640.79210.06770.073767.880431.006578.0153
248.9205-3.4019-4.2274.60762.90338.409-0.2084-0.8838-0.80090.2804-0.2460.2452-0.0172-0.14570.45440.6215-0.04260.07220.97950.1280.150357.330619.6889108.4618
2533.210512.02115.997411.70120.195311.96790.97890.3180.3322-0.3879-1.437-0.23051.00871.32830.45820.91060.04440.07070.558-0.02250.653262.829723.837293.3316
265.8412.2234-0.99797.49870.766511.4693-0.44640.6324-0.3111-0.1480.25990.27890.27630.27150.18650.3059-0.03290.20180.85870.09720.381857.942626.0128-1.6705
276.26040.542-2.13365.6030.44737.9010.0236-0.13060.05470.2350.0298-0.2201-0.14580.4678-0.05340.33570.02040.13210.93090.11480.136374.258436.95344.9515
286.53441.11091.83282.04340.08987.569-0.1738-1.07880.4436-0.016-0.2316-0.05760.1917-1.28870.40540.33380.05640.15361.13580.01260.153745.177126.959829.3102
2910.29693.03080.19236.3659-0.32676.0720.21820.3346-0.53190.1875-0.1007-0.8181-0.07140.5514-0.11750.46290.26080.03410.91630.21230.292678.373819.5225.3895
309.4622-5.13018.64243.5927-5.30458.37350.0420.32950.120.0939-0.4591-0.51740.01890.54950.41710.78130.0897-0.11130.7170.10020.864461.919921.688527.4854
3110.2618-1.10861.87616.64430.145910.4718-0.501-1.61760.54080.55280.24050.0226-0.5440.77880.26040.24140.0543-0.14221.37430.06450.448192.529974.0985109.1743
3210.8805-1.3823-0.2726.3743.052612.4387-0.43790.4617-0.7778-0.14410.3354-0.36060.24970.50540.10260.15140.0773-0.02561.00140.16170.4894102.340164.77393.4186
3310.36712.77650.54273.3763-1.04034.505-0.3781-0.15920.0024-0.1004-0.08860.03450.1203-0.5680.46670.14250.097-0.02420.7826-0.06610.312363.699169.22892.0676
349.3955-3.272-3.12472.66530.82575.36250.03480.66540.5909-0.3274-0.3338-0.437-0.58030.77220.29890.3247-0.23-0.10121.05690.2240.369491.088482.791276.4238
3523.6476-3.4507-12.34229.715313.635911.23660.92060.60351.13240.0801-0.3015-0.265-0.377-0.4105-0.61910.7998-0.0618-0.05860.60360.16080.644577.057577.590784.1401
3611.20040.4831-1.2464.5785-0.62699.6297-0.0773-0.3263-0.5115-0.4249-0.0322-0.0686-0.2867-1.24550.10960.22430.09750.10261.16840.07830.321-15.361657.372290.3794
3711.7301-0.81191.19687.1607-0.06078.4732-0.1903-1.2388-0.94820.7310.20830.27650.8378-0.4033-0.01790.31710.01120.14481.10620.35850.4106-5.232849.3088106.8351
386.59080.5489-1.56511.71520.33056.42930.0850.2676-0.39220.11180.1725-0.1493-0.20940.2053-0.25750.16060.0070.0910.67220.06390.280914.085459.991174.5272
397.7666-1.32111.11896.31-1.253910.35950.0697-1.11010.36460.14770.0571-0.0065-0.82840.4431-0.12680.44550.00590.16991.1394-0.19570.149511.386370.5506107.102
4037.0872-6.5106-38.61369.832710.292541.6291.42620.5078-0.2060.2312-1.3879-0.5234-1.2878-1.0005-0.03830.6860.0031-0.02610.7652-0.06960.706812.657366.881390.7233
419.90852.80842.10055.213-0.544511.89850.21761.56720.9475-0.2935-0.0969-0.59850.0488-0.1638-0.12070.28490.10660.21331.07680.10050.516953.886771.53865.7345
427.75260.5888-2.69396.8155-1.95017.83440.04421.5497-0.845-0.5694-0.09040.66270.8867-0.9130.04610.4399-0.1742-0.0221.7509-0.42540.238236.310459.99473.9252
437.66831.1051-0.81662.9582-3.047.785-0.1247-1.8356-0.48870.0475-0.1264-0.12270.01870.90320.25110.13320.05720.02791.3664-0.09990.188653.723165.394838.6865
447.65772.93822.31356.88422.12239.68720.06540.61541.34880.01590.08110.4395-0.2269-1.1449-0.14650.21660.11460.15141.1670.16190.458224.590874.828423.1473
458.809613.475-2.202530.5311-9.35274.8199-0.72060.3152-0.3884-0.78360.0885-0.57410.4193-0.25430.63210.7804-0.0430.15630.7541-0.21150.873939.002571.664931.3052
468.12491.39151.5457.74690.86948.4461-0.11070.31560.0606-0.63560.26810.1442-0.1983-0.701-0.15740.17680.0575-0.01020.833-0.17520.1747-5.189462.82527.8107
4710.60372.52881.85586.7864-0.43617.7295-0.4603-0.3942-0.54840.40250.07850.47910.8561-0.66820.38180.394-0.26090.13370.9082-0.15240.5799-13.363450.318742.6716
487.15870.0749-0.5821.56590.01463.3725-0.12230.0747-0.18830.0189-0.0816-0.04810.15260.50660.20390.12420.01930.03770.7624-0.10220.17923.744862.040745.1112
4910.99985.2374-3.93478.272-3.57557.22340.0742-0.20410.01630.39770.08020.2256-0.2083-0.6658-0.15440.26010.11840.04340.7047-0.12450.2023-5.430668.395761.4614
508.03688.91699.977427.529711.818625.60750.9244-0.04811.21870.0183-1.3849-0.38270.9872-1.07180.46050.63790.12130.11980.4746-0.03270.61758.914866.706253.4582
5117.68932.24024.28034.63932.70269.01160.20630.1178-0.98450.10950.3199-1.0180.00360.6646-0.52620.08630.06660.06440.9620.28180.4336102.725775.928246.2328
5211.15040.48035.00379.4816-1.23187.07021.74121.083-2.9068-1.5077-0.1069-0.53851.42791.1435-1.63430.81650.2789-0.05791.4127-0.32371.341895.830565.442129.6894
534.2933-2.942710.036510.3894-5.499823.835-0.6182-0.61080.16440.66470.4413-1.0373-1.5468-1.08480.17690.2113-0.13440.03061.88370.22220.287286.54973.868461.1673
542.5294-0.4464-0.40154.56711.86250.85820.2091-0.0934-0.080.4976-0.50960.39920.2354-0.16570.30060.1132-0.03580.11841.67120.23830.233970.68966.386562.396
556.1640.5005-1.57855.87213.579811.5961-0.2342-0.3642-0.1165-0.30540.24260.1644-0.21280.0487-0.00840.0567-0.11520.00140.47390.01410.018772.77979.666930.5207
560.0630.5902-0.73310.5163-3.239912.01840.03850.0193-0.03071.49020.844-1.8442-0.0458-0.1251-0.88260.81590.0016-0.04720.9838-0.0821.108573.064275.396246.3851
578.66171.09740.98674.6743-4.897211.54650.3407-1.2246-0.03160.4883-0.37230.37590.3165-0.40880.03160.6205-0.1580.11271.94540.03860.160833.065865.1338131.7761
585.8652-0.674-0.48269.3891-0.79864.46060.1747-0.9854-0.72040.4741-0.3417-1.31740.69681.20750.1670.72680.0396-0.15192.19490.32760.331552.983959.3494131.9058
595.86831.29891.05221.2921.49116.7375-0.1409-0.193-0.2104-0.0230.0851-0.04020.0674-0.60370.05580.11620.00150.07221.12120.04150.161233.863363.884698.0798
609.9301-2.2229-0.2445.96620.47236.25640.3272-1.16920.78230.6375-0.5135-0.1878-0.62361.12260.18630.3596-0.1991-0.08551.3669-0.22030.207959.368579.3851115.0713
617.94138.475712.52089.287814.256623.14-0.5228-0.53160.6202-0.5293-0.54030.7024-0.7229-0.45231.06310.74820.0180.01950.8788-0.09370.861746.328473.198106.3945
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A23 - 107
2X-RAY DIFFRACTION2A108 - 200
3X-RAY DIFFRACTION3A201 - 371
4X-RAY DIFFRACTION4A372 - 459
5X-RAY DIFFRACTION5A800
6X-RAY DIFFRACTION6B21 - 107
7X-RAY DIFFRACTION7B108 - 200
8X-RAY DIFFRACTION8B201 - 371
9X-RAY DIFFRACTION9B372 - 459
10X-RAY DIFFRACTION10B800
11X-RAY DIFFRACTION11C23 - 107
12X-RAY DIFFRACTION12C108 - 200
13X-RAY DIFFRACTION13C201 - 371
14X-RAY DIFFRACTION14C372 - 460
15X-RAY DIFFRACTION15C800
16X-RAY DIFFRACTION16D22 - 107
17X-RAY DIFFRACTION17D108 - 200
18X-RAY DIFFRACTION18D201 - 371
19X-RAY DIFFRACTION19D372 - 460
20X-RAY DIFFRACTION20D800
21X-RAY DIFFRACTION21E23 - 107
22X-RAY DIFFRACTION22E108 - 200
23X-RAY DIFFRACTION23E201 - 371
24X-RAY DIFFRACTION24E372 - 461
25X-RAY DIFFRACTION25E800
26X-RAY DIFFRACTION26F23 - 107
27X-RAY DIFFRACTION27F108 - 200
28X-RAY DIFFRACTION28F201 - 371
29X-RAY DIFFRACTION29F372 - 460
30X-RAY DIFFRACTION30F800
31X-RAY DIFFRACTION31G23 - 107
32X-RAY DIFFRACTION32G108 - 200
33X-RAY DIFFRACTION33G201 - 371
34X-RAY DIFFRACTION34G372 - 459
35X-RAY DIFFRACTION35G800
36X-RAY DIFFRACTION36H22 - 107
37X-RAY DIFFRACTION37H108 - 200
38X-RAY DIFFRACTION38H201 - 371
39X-RAY DIFFRACTION39H372 - 459
40X-RAY DIFFRACTION40H800
41X-RAY DIFFRACTION41I21 - 107
42X-RAY DIFFRACTION42I108 - 200
43X-RAY DIFFRACTION43I201 - 371
44X-RAY DIFFRACTION44I372 - 459
45X-RAY DIFFRACTION45I800
46X-RAY DIFFRACTION46J21 - 107
47X-RAY DIFFRACTION47J108 - 200
48X-RAY DIFFRACTION48J201 - 371
49X-RAY DIFFRACTION49J372 - 462
50X-RAY DIFFRACTION50J800
51X-RAY DIFFRACTION51K23 - 107
52X-RAY DIFFRACTION52K108 - 200
53X-RAY DIFFRACTION53K201 - 238
54X-RAY DIFFRACTION54K239 - 369
55X-RAY DIFFRACTION55K370 - 459
56X-RAY DIFFRACTION56K800
57X-RAY DIFFRACTION57L23 - 107
58X-RAY DIFFRACTION58L108 - 200
59X-RAY DIFFRACTION59L201 - 371
60X-RAY DIFFRACTION60L372 - 459
61X-RAY DIFFRACTION61L800

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